ELISA的方法檢測(cè)多肽與小鼠前列腺特異性抗原(PSA)測(cè)定試劑盒的結(jié)合情況。準(zhǔn)備將合成多肽(其長(zhǎng)度大約為8~10個(gè)氨基酸)包被于ELISA板,再加入小鼠前列腺特異性抗原(PSA)測(cè)定試劑盒,一抗、酶標(biāo)二抗。請(qǐng)問(wèn)在包被的過(guò)程中應(yīng)采取哪種包被緩沖液?固相載體是否要特殊處理?封閉液用BSA可以嗎?
1、多肽抗原的包被一般需先使其與無(wú)關(guān)蛋白質(zhì)如牛血清白蛋白質(zhì)(BSA)等偶聯(lián),借助于偶聯(lián)物與固相載體的吸附,間接地結(jié)合到固相載體表面。
2、小鼠前列腺特異性抗原(PSA)測(cè)定試劑盒包被用抗原或抗體的濃度,包被的溫度和時(shí)間,包被液的pH等應(yīng)根據(jù)試驗(yàn)的特點(diǎn)和材料的性質(zhì)而選定??贵w和蛋白質(zhì)抗原一般采用pH9.6的碳酸鹽緩沖液作為稀釋液,也有用pH7.2的磷酸鹽緩沖液及pH7~8的Tris-HCL緩沖液作為稀釋液的。通常在ELISA板孔中加入包被液后,在4-8℃冰箱中放置過(guò)夜,37℃中保溫2小時(shí)被認(rèn)為具有同等的包被效果。
3、zui常用的封閉劑是0.05%-0.5%的牛血清白蛋白,小鼠前列腺特異性抗原(PSA)測(cè)定試劑盒也有用10%的小牛血清或1%明膠作為封閉劑的。脫脂奶粉也是一種良好的封閉劑,其zui大的特點(diǎn)是價(jià)廉,可以高濃度使用(5%)。
顯色終止后出現(xiàn)黑色絮狀沉淀可能為包被板上的蛋白,酶標(biāo)記物,檢測(cè)物,封閉液中蛋白變性以及顯色反應(yīng)TMB過(guò)量,不溶,沉淀出來(lái)。
小鼠前列腺特異性抗原(PSA)測(cè)定試劑盒其它優(yōu)勢(shì)產(chǎn)品如下:
klA573Mu 小鼠Ⅲ型前膠原氨基端原肽(PⅢNP)ELISA試劑盒 ELISA Kit for Procollagen III N-Terminal Propeptide (PIIINP) Assay range:30 ng/L -1161 ng/L
klA604Mu 小鼠載脂蛋白A2(APOA2)ELISA試劑盒 ELISA Kit for Apolipoprotein A2 (APOA2) Assay range:30 ng/L -1162 ng/L
klA574Mu 小鼠層粘連蛋白α1(LAMα1)ELISA試劑盒 ELISA Kit for Laminin Alpha 1 (LAMa1) Assay range:30 ng/L -1163 ng/L
klA801Mu 小鼠堿性鞘磷脂磷酸二酯酶(Alk-Smase)ELISA試劑盒 ELISA Kit for Alkaline Sphingomyelinase (Alk-Smase) Assay range:30 ng/L -1164 ng/L
klA802Mu 小鼠膽囊收縮素(CCK)ELISA試劑盒 ELISA Kit for Cholecystokinin (CCK) Assay range:30 ng/L -1165 ng/L
klA804Mu 小鼠胰高血糖素樣肽1(GLP1)ELISA試劑盒 ELISA Kit for Glucagon Like Peptide 1 (GLP1) Assay range:30 ng/L -1166 ng/L
klA799Mu 小鼠骨成型蛋白7(BMP7)ELISA試劑盒 ELISA Kit for Bone Morphogenetic Protein 7 (BMP7) Assay range:30 ng/L -1167 ng/L
klA839Mu 小鼠促甲狀腺素釋放激素(TRH)ELISA試劑盒 ELISA Kit for Thyrotropin Releasing Hormone (TRH) Assay range:30 ng/L -1168 ng/L
klA843Mu 小鼠*釋放激素(GnRH)ELISA試劑盒 ELISA Kit for Gonadotropin Releasing Hormone (GnRH) Assay range:30 ng/L -1169 ng/L
klA993Mu 小鼠促微管蛋白聚合蛋白(TPPP)ELISA試劑盒 ELISA Kit for Tubulin Polymerization Promoting Protein (TPPP) Assay range:30 ng/L -1170 ng/L
klA855Mu 小鼠核因子κB受體激活因子配基(RANκL)ELISA試劑盒 ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Assay range:30 ng/L -1171 ng/L
klA372Mu 小鼠凝溶膠蛋白(GS)ELISA試劑盒 ELISA Kit for Gelsolin (GS) Assay range:30 ng/L -1172 ng/L
klA860Mu 小鼠上皮中性粒細(xì)胞激活肽78(ENA78)ELISA試劑盒 ELISA Kit for Epithelial Neutrophil Activating Peptide 78 (ENA78) Assay range:30 ng/L -1173 ng/L
klA371Mu 小鼠10kDa干擾素γ誘導(dǎo)蛋白(IP10)ELISA試劑盒 ELISA Kit for Interferon Gamma Induced Protein 10kDa (IP10) Assay range:30 ng/L -1174 ng/L
klA373Mu 小鼠結(jié)蛋白(Des)ELISA試劑盒 ELISA Kit for Desmin (Des) Assay range:30 ng/L -1175 ng/L
klB347Mu 小鼠垂體腺苷酸環(huán)化酶激活肽(ADCYAP1)ELISA試劑盒 ELISA Kit for Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) Assay range:30 ng/L -1176 ng/L
klB867Mu 小鼠表皮生長(zhǎng)因子受體2(EGFR2)ELISA試劑盒 ELISA Kit for Epidermal Growth Factor Receptor 2 (EGFR2) Assay range:30 ng/L -1177 ng/L
klA684Mu 小鼠粘蛋白5B(MUC5B)ELISA試劑盒 ELISA Kit for Mucin 5 Subtype B (MUC5B) Assay range:30 ng/L -1178 ng/L
klB980Mu 小鼠白介素33(IL33)ELISA試劑盒 ELISA Kit for Interleukin 33 (IL33) Assay range:30 ng/L -1179 ng/L
klB154Mu 小鼠纖維蛋白原α(FGα)ELISA試劑盒 ELISA Kit for Fibrinogen Alpha (FGa) Assay range:30 ng/L -1180 ng/L
klB897Mu 小鼠胰島素降解酶(IDE)ELISA試劑盒 ELISA Kit for Insulin Degrading Enzyme (IDE) Assay range:30 ng/L -1181 ng/L
klA753Mu 小鼠Toll樣受體4(TLR4)ELISA試劑盒 ELISA Kit for Toll Like Receptor 4 (TLR4) Assay range:30 ng/L -1182 ng/L
klA663Mu 小鼠Toll樣受體2(TLR2)ELISA試劑盒 ELISA Kit for Toll Like Receptor 2 (TLR2) Assay range:30 ng/L -1183 ng/L
klA295Mu 小鼠*過(guò)氧化酶1(GPX1)ELISA試劑盒 ELISA Kit for Glutathione Peroxidase 1 (GPX1) Assay range:30 ng/L -1184 ng/L
klB091Mu 小鼠肝腎骨堿性磷酸酶(ALPL)ELISA試劑盒 ELISA Kit for Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) Assay range:30 ng/L -1185 ng/L
klA479Mu 小鼠肌酸激酶MB同工酶(CKMB)ELISA試劑盒 ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB) Assay range:30 ng/L -1186 ng/L
klB697Mu 小鼠α1-抗*(α1AT)ELISA試劑盒 ELISA Kit for Alpha-1-Antitrypsin (a1AT) Assay range:30 ng/L -1187 ng/L
klB866Mu 小鼠神經(jīng)調(diào)節(jié)素1(NRG1)ELISA試劑盒 ELISA Kit for Neuregulin 1 (NRG1) Assay range:30 ng/L -1188 ng/L
klB949Mu 小鼠轉(zhuǎn)化生長(zhǎng)因子β3(TGFβ3)ELISA試劑盒 ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3) Assay range:30 ng/L -1189 ng/L