ELISA的方法檢測多肽與小鼠血紅素氧合酶1(HO-1)測定試劑盒的結(jié)合情況。準(zhǔn)備將合成多肽(其長度大約為8~10個氨基酸)包被于ELISA板,再加入小鼠血紅素氧合酶1(HO-1)測定試劑盒,一抗、酶標(biāo)二抗。請問在包被的過程中應(yīng)采取哪種包被緩沖液?固相載體是否要特殊處理?封閉液用BSA可以嗎?
1、多肽抗原的包被一般需先使其與無關(guān)蛋白質(zhì)如牛血清白蛋白質(zhì)(BSA)等偶聯(lián),借助于偶聯(lián)物與固相載體的吸附,間接地結(jié)合到固相載體表面。
2、小鼠血紅素氧合酶1(HO-1)測定試劑盒包被用抗原或抗體的濃度,包被的溫度和時間,包被液的pH等應(yīng)根據(jù)試驗(yàn)的特點(diǎn)和材料的性質(zhì)而選定??贵w和蛋白質(zhì)抗原一般采用pH9.6的碳酸鹽緩沖液作為稀釋液,也有用pH7.2的磷酸鹽緩沖液及pH7~8的Tris-HCL緩沖液作為稀釋液的。通常在ELISA板孔中加入包被液后,在4-8℃冰箱中放置過夜,37℃中保溫2小時被認(rèn)為具有同等的包被效果。
3、zui常用的封閉劑是0.05%-0.5%的牛血清白蛋白,小鼠血紅素氧合酶1(HO-1)測定試劑盒也有用10%的小牛血清或1%明膠作為封閉劑的。脫脂奶粉也是一種良好的封閉劑,其zui大的特點(diǎn)是價廉,可以高濃度使用(5%)。
顯色終止后出現(xiàn)黑色絮狀沉淀可能為包被板上的蛋白,酶標(biāo)記物,檢測物,封閉液中蛋白變性以及顯色反應(yīng)TMB過量,不溶,沉淀出來。
小鼠血紅素氧合酶1(HO-1)測定試劑盒其它優(yōu)勢產(chǎn)品如下:
klA957Mu 小鼠Ⅰ型前膠原氨基端原肽(PⅠNP)ELISA試劑盒 ELISA Kit for Procollagen I N-Terminal Propeptide (PINP) Assay range:30 ng/L -1286 ng/L
klA282Mu 小鼠組蛋白H2A(H2A)ELISA試劑盒 ELISA Kit for Histone H2A (H2A) Assay range:30 ng/L -1287 ng/L
klA308Mu 小鼠半乳糖凝集素8(GAL8)ELISA試劑盒 ELISA Kit for Galectin 8 (GAL8) Assay range:30 ng/L -1288 ng/L
klA312Mu 小鼠半乳糖凝集素12(GAL12)ELISA試劑盒 ELISA Kit for Galectin 12 (GAL12) Assay range:30 ng/L -1289 ng/L
klA315Mu 小鼠半乳糖凝集素6(GAL6)ELISA試劑盒 ELISA Kit for Galectin 6 (GAL6) Assay range:30 ng/L -1290 ng/L
klA321Mu 小鼠半乳糖凝集素1(GAL1)ELISA試劑盒 ELISA Kit for Galectin 1 (GAL1) Assay range:30 ng/L -1291 ng/L
klA322Mu 小鼠基質(zhì)金屬蛋白酶15(MMP15)ELISA試劑盒 ELISA Kit for Matrix Metalloproteinase 15 (MMP15) Assay range:30 ng/L -1292 ng/L
klA293Mu 小鼠*脫氫酶(GDH)ELISA試劑盒 ELISA Kit for Glutamate Dehydrogenase 1 (GDH) Assay range:30 ng/L -1293 ng/L
klA012Mu 小鼠S100鈣結(jié)合蛋白(S100)ELISA試劑盒 ELISA Kit for S100 Calcium Binding Protein (S100) Assay range:30 ng/L -1294 ng/L
klA413Mu 小鼠粘蛋白1(MUC1)ELISA試劑盒 ELISA Kit for Mucin 1 (MUC1) Assay range:30 ng/L -1295 ng/L
klA431Mu 小鼠顆粒酶M(GZMM)ELISA試劑盒 ELISA Kit for Granzyme M (GZMM) Assay range:30 ng/L -1296 ng/L
klC886Mu 小鼠突觸囊泡蛋白Ⅰ(SYT1)ELISA試劑盒 ELISA Kit for Synaptotagmin I (SYT1) Assay range:30 ng/L -1297 ng/L
klC955Mu 小鼠25kDa突觸關(guān)聯(lián)蛋白(SNAP25)ELISA試劑盒 ELISA Kit for Synaptosomal Associated Protein 25kDa (SNAP25) Assay range:30 ng/L -1298 ng/L
klA675Mu 小鼠*Ⅴ(F5)ELISA試劑盒 ELISA Kit for Coagulation Factor V (F5) Assay range:30 ng/L -1299 ng/L
klB070Mu 小鼠類*(TPS)ELISA試劑盒 ELISA Kit for Tryptase (TPS) Assay range:30 ng/L -1300 ng/L
klC313Mu 小鼠抗*(AT)ELISA試劑盒 ELISA Kit for Antithrombin (AT) Assay range:30 ng/L -1301 ng/L
klB050Mu 小鼠雌激素受體α(ERα)ELISA試劑盒 ELISA Kit for Estrogen Receptor Alpha (ERa) Assay range:30 ng/L -1302 ng/L
klB252Mu 小鼠雄激素受體(AR)ELISA試劑盒 ELISA Kit for Androgen Receptor (AR) Assay range:30 ng/L -1303 ng/L
klB354Mu 小鼠芳香烴受體(AhR)ELISA試劑盒 ELISA Kit for Aryl Hydrocarbon Receptor (AhR) Assay range:30 ng/L -1304 ng/L
klB222Mu 小鼠突觸核蛋白α(SNCα)ELISA試劑盒 ELISA Kit for Synuclein Alpha (SNCa) Assay range:30 ng/L -1305 ng/L
klB690Mu 小鼠滲透性糖蛋白(Pgp)ELISA試劑盒 ELISA Kit for Permeability Glycoprotein (Pgp) Assay range:30 ng/L -1306 ng/L
klB969Mu 小鼠補(bǔ)體成分5(C5)ELISA試劑盒 ELISA Kit for Complement Component 5 (C5) Assay range:30 ng/L -1307 ng/L
klC156Mu 小鼠Ⅹ型膠原(COL10)ELISA試劑盒 ELISA Kit for Collagen Type X (COL10) Assay range:30 ng/L -1308 ng/L
klB933Mu 小鼠組織蛋白酶S(CTSS)ELISA試劑盒 ELISA Kit for Cathepsin S (CTSS) Assay range:30 ng/L -1309 ng/L
klA888Mu 小鼠補(bǔ)體成分4(C4)ELISA試劑盒 ELISA Kit for Complement Component 4 (C4) Assay range:30 ng/L -1310 ng/L
klA738Mu 小鼠激活蛋白C(APC)ELISA試劑盒 ELISA Kit for Activated Protein C (APC) Assay range:30 ng/L -1311 ng/L
klB190Mu 小鼠白介素19(IL19)ELISA試劑盒 ELISA Kit for Interleukin 19 (IL19) Assay range:30 ng/L -1312 ng/L
klC032Mu 小鼠白介素22(IL22)ELISA試劑盒 ELISA Kit for Interleukin 22 (IL22) Assay range:30 ng/L -1313 ng/L
klC064Mu 小鼠白介素24(IL24)ELISA試劑盒 ELISA Kit for Interleukin 24 (IL24) Assay range:30 ng/L -1314 ng/L
klB451Mu 小鼠早期生長應(yīng)答因子2(EGR2)ELISA試劑盒 ELISA Kit for Early Growth Response Protein 2 (EGR2) Assay range:30 ng/L -1315 ng/L
klA402Mu 小鼠基質(zhì)金屬蛋白酶12(MMP12)ELISA試劑盒 ELISA Kit for Matrix Metalloproteinase 12 (MMP12) Assay range:30 ng/L -1316 ng/L
klB039Mu 小鼠表面活性物質(zhì)關(guān)聯(lián)蛋白D(SPD)ELISA試劑盒 ELISA Kit for Surfactant Associated Protein D (SPD) Assay range:30 ng/L -1317 ng/L
klC284Mu 小鼠α白蛋白(AFM)ELISA試劑盒 ELISA Kit for Afamin (AFM) Assay range:30 ng/L -1318 ng/L
klA260Mu 小鼠β2-微球蛋白(β2M)ELISA試劑盒 ELISA Kit for Beta-2-Microglobulin (b2M) Assay range:30 ng/L -1319 ng/L
klA608Mu 小鼠微粒體*S轉(zhuǎn)移酶1(MGST1)ELISA試劑盒 ELISA Kit for Microsomal Glutathione S Transferase 1 (MGST1) Assay range:30 ng/L -1320 ng/L
klA305Mu 小鼠甲狀腺結(jié)合球蛋白(TBG)ELISA試劑盒 ELISA Kit for Thyroxine Binding Globulin (TBG) Assay range:30 ng/L -1321 ng/L
klA425Mu 小鼠突觸素(SYP)ELISA試劑盒 ELISA Kit for Synaptophysin (SYP) Assay range:30 ng/L -1322 ng/L
klA613Mu 小鼠*S轉(zhuǎn)移酶θ2(GSTθ2)ELISA試劑盒 ELISA Kit for Glutathione S Transferase Theta 2 (GSTt2) Assay range:30 ng/L -1323 ng/L