牛免疫球蛋白產(chǎn)品描述
Bovine IgG是從混合的正常血清中,使用分離技術(shù)純化而獲得的純化免疫球蛋白(Ig),并zui終經(jīng)凍干而以粉末形式供應(yīng),該產(chǎn)品未經(jīng)任何標記,為非特異性IgG。經(jīng)SDS-PAGE分析該IgG的純度>97%。
緩沖液配方:0.01M Phosphate buffer in 0.15M NaCl。
溶解方法 牛免疫球蛋白
使用時,將其溶解到無菌水或緩沖液中(pH7.2 ± 0.2)。作為對照IgG使用時,用量和其它IgG的用量應(yīng)該相同。對照IgG用量過多可能會導(dǎo)致背景過高。
應(yīng)用范圍
1)可作為聚乙二醇或硫酸銨免疫沉淀步驟中的共沉淀試劑;
2)作為蛋白質(zhì)穩(wěn)定劑;
3)用作大多數(shù)免疫分析實驗中的封閉試劑;
4)在一些生化分析測試中,也可以作為IgG對照。作為對照IgG時,可以排除IgG本身和特定目的蛋白或其他特定生物分子的非特異性結(jié)合;
5)參考抗原,如藥物試驗中觀察免疫效果,用IgG作參考抗原。
運輸與保存方法
冰袋(wet ice)運輸。凍干粉可儲存在2-8℃。溶解后,儲存于-20 oC,建議分裝保存,避免反復(fù)凍融。
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