Anti-IGSF11抗體,大腦和睪特異性免疫球蛋白超家族成員11抗體一抗孵育(Primary antibody incubation):
參考一抗的說明書,按照適當比例用Western一抗稀釋液稀釋一抗。
用微型臺式真空泵吸盡封閉液,立即加入稀釋好的一抗,室溫或4℃在側(cè)擺搖床上緩慢搖動孵育一小時。如果一抗孵育一小時效果不佳,可以在4℃緩慢搖動孵育過夜?;厥找豢?。加入Western洗滌液,在側(cè)擺搖床上緩慢搖動洗滌5-10分鐘。吸盡洗滌液后,再加入洗滌液,洗滌5-10分鐘。共洗滌3次。如果結(jié)果背景較高可以適當延長洗滌時間并增加洗滌次數(shù)。
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產(chǎn)品名稱:Anti-IGSF11抗體,大腦和睪特異性免疫球蛋白超家族成員11抗體
產(chǎn)品編號:BYK-9201R
英文名稱:Anti-IGSF11
中文名稱:大腦和睪特異性免疫球蛋白超家族成員11抗體
英文別名:brain and testis specific immunoglobin superfamily protein; BTIGSF; CXADR like 1; CXADRL1; Igsf13; immunoglobulin superfamily member 11; Immunoglobulin superfamily member 11 precursor; MGC35227; V set and immunoglobulin domain containing 3; V set and immunoglobulin domain containing protein 3; VSIG3; IGS11_HUMAN.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細胞術(shù)等實驗(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-IGSF11抗體,大腦和睪特異性免疫球蛋白超家族成員11抗體
英文介紹:IGSF11 is also known as BTIGSF (brain and testis-specific immunoglobulin superfamily protein) or VSIG3 (V-set and immunoglobulin domain-containing protein 3) and is a 431 amino acid protein that is expressed as three isoforms. IGSF11 is highly expressed in testis and ovary and is also expressed in brain, kidney and skeletal muscle, localized to the cellular membrane as a single-pass membrane protein. IGSF11 is an immunoglobulin with V-type and C2-type domains that function in molecular recognition. When IGSF11 is in the trans position, it plays an important role in cell-cell adhesion via both homophilic and heterophilic interactions with other molecules. These cell–cell interactions are also thought to be important for neuronal cell interactions, such as neuron–neuron or neuron–glia interactions, which are important for the development and function of the central nervous system. In addition, IGSF11 might also be involved interactions between Sertoli cells and spermatocytes, which are important associations during spermatogenesis. The IGSF11 gene is commonly upregulated in gastric cancer and IGSF11 is highly expressed in many types of human tumors, indicating that it may be useful as a target for immunotherapy.
此產(chǎn)品本公司有標記的一抗出售,相關(guān)標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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