Anti-IQCG抗體,IQCG蛋白抗體一抗孵育(Primary antibody incubation):
參考一抗的說(shuō)明書(shū)，按照適當(dāng)比例用Western一抗稀釋液稀釋一抗。
用微型臺(tái)式真空泵吸盡封閉液，立即加入稀釋好的一抗，室溫或4℃在側(cè)擺搖床上緩慢搖動(dòng)孵育一小時(shí)。如果一抗孵育一小時(shí)效果不佳，可以在4℃緩慢搖動(dòng)孵育過(guò)夜。回收一抗。加入Western洗滌液，在側(cè)擺搖床上緩慢搖動(dòng)洗滌5-10分鐘。吸盡洗滌液后，再加入洗滌液，洗滌5-10分鐘。共洗滌3次。如果結(jié)果背景較高可以適當(dāng)延長(zhǎng)洗滌時(shí)間并增加洗滌次數(shù)。
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產(chǎn)品名稱(chēng)：Anti-IQCG抗體,IQCG蛋白抗體
產(chǎn)品編號(hào)：BYK-9022R
英文名稱(chēng)：Anti-IQCG
中文名稱(chēng)：IQCG蛋白抗體
英文別名：IQ domain containing protein G; IQ motif containing G; DKFZp434B227; FLJ11667; FLJ23571; FLJ37775; IQCG_HUMAN.
產(chǎn)品規(guī)格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)（可來(lái)電索取說(shuō)明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-IQCG抗體,IQCG蛋白抗體
英文介紹：IQCG is a 443 amino acid protein containing one IQ domain. Widely distributed in nature, the IQ domain forms an amphiphilic seven-turn α-helix capable of binding calmodulin in a Ca2+-independent manner. The level of intracellular calcium is tightly regulated in all eukaryotic cells. A modest increase in this level can result in a myriad of physiological responses, most of which are mediated by calmodulin (CaM), the universal calcium sensor. In acute T-lymphoid/myeloid leukemia, IQCG forms a complex with Nup98, an O-linked glycoprotein and a component of the nuclear pore complex. NUP98-IQCG complex bind co-activators and/or co-repressors, which suggest a role in transcriptional regulation.Nup98-IQCG complex inhibits 32Dcl3 cell apoptosis induced by Arabinofuranosylcytosine (Ara-C) and partially blocks granulocyte differentiation induced by G-CSF. IQCG exists as two isoforms due to alternatively splicing events.
此產(chǎn)品本公司有標(biāo)記的一抗出售，相關(guān)標(biāo)記有：Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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