非干擾型蛋白濃度測定試劑盒ZIKER-子科現(xiàn)貨
英文名:Non-Interference Protein Assay Kit
相關(guān)類別:蛋白定量
儲存:常溫下運輸,收到后,沉淀試劑一和沉淀試劑二常溫下保存,將生色試劑B~20°C避光保存和蛋白標準BSA于-20°C保存,生色試劑A于2~8°C保存,保質(zhì)期一年。
產(chǎn)品簡介
非干擾型蛋白質(zhì)定量試劑盒是排除決大多數(shù)實驗室常規(guī)試劑的干擾的光學(xué)定量方法,它可以去除離子、非離子、兩性去污試劑等、還原試劑、螯合試劑、Tris、糖、兩性電解質(zhì)、尿素、硫尿、鹽酸胍等變性試劑,對于在Laemmeli buffer等蛋白質(zhì)上樣緩沖液,2D Sample Extraction Buffer、高濃度的β~mercaptoethanol (<15%)和脂質(zhì)體中的蛋白質(zhì)也可以定量。蛋白質(zhì)量在0.5~50 μg之間有良好的線性關(guān)系,需要的樣品體積在1~50 μL,特殊的沉淀試劑可以去除掉絕大多數(shù)干擾物質(zhì),被沉淀的蛋白質(zhì)與含有銅的堿性物質(zhì)相混合,未與蛋白質(zhì)相結(jié)合銅在被還原為一價后與特定的生色試劑反應(yīng),產(chǎn)生特定的480 nm吸收波長。所以隨著蛋白質(zhì)的濃度增加,未與蛋白質(zhì)結(jié)合銅相應(yīng)減少,這樣特定的吸收波長的吸收值與溶液中的蛋白質(zhì)濃度成反比,這種蛋白質(zhì)定量方法不受氨基酸副鏈和蛋白質(zhì)的種類的影響,本試劑盒可以做250次微量定量。
非干擾型蛋白濃度測定試劑盒ZIKER-子科現(xiàn)貨產(chǎn)品特點
1. 整個實驗過程大約需要45 min左右。
2. 定量過程不受到非蛋白的多種干擾因素影響。
3. 定量范圍在0.5~50 μg。
4. 適合對各種生物的細胞或組織裂解液或2D電泳細胞裂解液中樣品的直接進行定量。
是子科生物的重點推廣產(chǎn)品,如需購買的話我公司有專業(yè)的銷售人員對您購買過程進行全程指導(dǎo),請放心購買,發(fā)貨時均會附上相應(yīng)的產(chǎn)品質(zhì)檢報告單,使用說明書和推薦用法用量,并提供國家正規(guī)機打發(fā)票,讓您體會無憂購物全程,且無售后煩憂!
深圳子科生物生化試劑產(chǎn)品,經(jīng)過長期廣大科研工作者的實驗認證,質(zhì)量穩(wěn)定,品種齊全,貨真價實,貨源充足,發(fā)貨周期快,是廣大科研工作者的*廠家,廣大生物經(jīng)銷商和科研工作者可以放心購買哦!
相關(guān)產(chǎn)品如下:RC-287 2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid, sodium salt 0.1 g 12 MONTHS
RC-288 2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid, sodium salt 0.025 g 12 MONTHS
RC-289 2,3,5-Triphenyltetrazolium chloride 1000 G 12 MONTHS
RC-290 2,3,5-Triphenyltetrazolium chloride 100 G 12 MONTHS
RC-291 2,3,5-Triphenyltetrazolium chloride 2500 G 12 MONTHS
RC-292 2,3,5-Triphenyltetrazolium chloride 250 G 12 MONTHS
RC-293 2,3,5-Triphenyltetrazolium chloride 500 G 12 MONTHS
RC-294 2-Naphthyl caprylate 1 G 12 MONTHS
RC-295 2-Naphthyl caprylate 0.5 G 12 MONTHS
RC-296 2-Naphthyl-alpha-D-glucopyranoside 1 G 12 MONTHS
RC-297 2-Naphthyl-alpha-D-glucopyranoside 2.5 G 12 MONTHS
RC-298 2-Naphthyl-alpha-D-glucopyranoside 0.5 G 12 MONTHS
RC-299 2-Naphthyl-alpha-L-fucopyranoside 1 G 12 MONTHS
RC-300 2-Naphthyl-beta-D-galactopyranoside 1 G 12 MONTHS
RC-301 2-Naphthyl-beta-D-galactopyranoside 10 G 12 MONTHS
RC-302 2-Naphthyl-beta-D-galactopyranoside 2.5 G 12 MONTHS
RC-303 2-Naphthyl-beta-D-galactopyranoside 50 G 12 MONTHS
RC-304 2-Nitrophenyl-1-thio-beta-D-galactopyranoside 1 G 12 MONTHS
RC-305 2-Nitrophenyl-beta-D-cellobioside 0.5 G 12 MONTHS
RC-306 2-Nitrophenyl-beta-D-glucopyranoside 5 G 12 MONTHS
RC-307 2-Nitrophenyl-N-acetyl-beta-D-glucosaminide 10 G 12 MONTHS
RC-308 3,3',5,5'-Tetramethylbenzidine, dihydrochloride hydrate 10 G 12 MONTHS
RC-309 3,3',5,5'-Tetramethylbenzidine, dihydrochloride hydrate 100 G 12 MONTHS
RC-310 3,3',5,5'-Tetramethylbenzidine, dihydrochloride hydrate 500 G 12 MONTHS
RC-311 3,3',5,5'-Tetramethylbenzidine, dihydrochloride hydrate 250 G 12 MONTHS
RC-312 3,3',5,5'-Tetramethylbenzidine, dihydrochloride hydrate 5 G 12 MONTHS
RC-313 3,3',5,5'-Tetramethylbenzidine, dihydrochloride hydrate 50 G 12 MONTHS
RC-314 3,3',5,5'-Tetramethylbenzidine, free base 1000 G 12 MONTHS
RC-315 3,3',5,5'-Tetramethylbenzidine, free base 100 G 12 MONTHS
RC-316 3,3',5,5'-Tetramethylbenzidine, free base 250 G 12 MONTHS
RC-317 3,3',5,5'-Tetramethylbenzidine, free base 50 G 12 MONTHS
RC-318 3,3',5,5'-Tetramethylbenzidine, free base 500 G 12 MONTHS
RC-319 3-Indoxyl butyrate 10 g 12 MONTHS
RC-320 3-Indoxyl phosphate, bis(2-amino-2-methyl-1,3-propanediol) salt 25 G 12 MONTHS