中文名稱：磷酸化整合素β2/Integrin β2抗體
英文名稱：Anti-Phospho-CD18(Ser756)
產(chǎn)品編號(hào)：BYK-10461R
別名：CD18 (Phospho Ser756); CD18 (Phospho-Ser756); CD18 (Phospho S756); p-CD18 (S756); p-CD18 (Ser756); Integrin beta 2; 95 subunit beta; CD 18; CD18; Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor); Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta; Cell surface adhesion glycoproteins LFA-1/CR3/p150; Complement receptor C3 beta subunit; Complement receptor C3 subunit beta; Integrin beta chain beta 2; Integrin beta-2; Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITB2_HUMAN; ITGB2; LAD; LCAMB; Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C; Leukocyte cell adhesion molecule CD18; LFA 1; LFA1; Lymphocyte function associated antigen 1; MAC 1; MAC1; MF17; MFI7; OTTHUMP00000115278; OTTHUMP00000115279; OTTHUMP00000115280; OTTHUMP00000115281; OTTHUMP00000115282.
產(chǎn)品介紹：The product of this gene belongs to the integrin beta chain family of proteins. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. This gene encodes the integrin beta chain beta 2. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene.
此產(chǎn)品本公司有標(biāo)記的一抗出售，相關(guān)標(biāo)記有：Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
規(guī)格：0.1ml/0.2ml
濃度：1mg/1ml
抗體來源：本公司抗體有兔來源和鼠來源等
克隆類型：兔來源為多抗，鼠來源為單抗
產(chǎn)品類型：一抗
Anti-Phospho-CD18(Ser756)抗體,磷酸化整合素β2/Integrin β2抗體保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
避免反復(fù)凍融，保質(zhì)期一年。
Anti-Phospho-CD18(Ser756)抗體,磷酸化整合素β2/Integrin β2抗體額外的條帶（Extra Bands）
可能存在的原因：一抗的非特性結(jié)合
解決方法:降低抗體濃度；減少總蛋白的上樣量；免疫親和層析純化抗體
可能存在的原因：二抗的非特異性結(jié)合
解決方法:跑一個(gè)二抗的陰性對(duì)照（不孵育一抗）；將二抗進(jìn)行免疫親和層析
可能存在的原因：一抗或者二抗的非異性結(jié)合
解決方法:添加0.1%-0.5%的吐溫20到抗體稀釋也中；用2%的脫脂奶粉溶解抗體，并適當(dāng)調(diào)整抗體濃度；增加洗膜液中NaCl的濃度（0.15M-0.5M）；增加洗膜時(shí)間或者次數(shù)；
可能存在的原因：蛋白聚急
解決方法:提高DTT的濃度，加樣前加熱煮沸5-10min
可能存在的原因：蛋白的降解
解決方法:避免反復(fù)凍融；添加蛋白酶抑制劑；制備新鮮樣品；
可能存在的原因：試劑污染
解決方法:配置新鮮的給中溶液
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