上海研盟生物科技有限公司Anti-KAT3B抗體,轉錄接頭蛋白EP300抗體*,主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取。:
中文名稱:轉錄接頭蛋白EP300抗體
英文名稱:Anti-KAT3B/Ep300抗體
產品編號:BYK-2678R
別名:CREBBP/EP300 inhibitory protein 1; Cyclic AMP responsive enhancer binding protein; E1A associated protein p300; E1A binding protein p300; EC 2.3.1.48; EP300; EP300: E1A binding protein p300; RB and P300 binding protein EID 1; Histone acetyltransferase p300; p300 HAT; Retinoblastoma protein associated protein.
此產品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來源:本公司抗體有兔來源和鼠來源等
克隆類型:兔來源為多抗,鼠來源為單抗
產品類型:一抗
Anti-KAT3B抗體,轉錄接頭蛋白EP300抗體產品介紹:This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. This gene contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene.
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.避免反復凍融,保質期一年。
上海研盟生物科技有限公司各種HRP酶標記成抗體的效價測定程序如下(如:羊抗鼠IgG-HRP的效價測定):
1、抗原包板:15ug/ml 小鼠IgG-pH9.6·CB液 0.1ml/孔;
2、37°C包被60min或4°C過夜;
3、PBST洗板3次,5min/次;
4、加入倍比稀釋的HRP標記抗體 0.1ml/孔;
5、37°C 30min;
6、PBST洗板5min/次×3;
7、底物液0.1ml/孔 37°C 5-10min;
8、終止液:2N H2SO4 0.05ml/孔;
9、450nm 測定OD值;
10、陽性OD值比對照值大3倍,為判斷效價稀釋度。
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