上海研盟生物科技有限公司Anti-Integrin alpha 4抗體,整合素α4抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說(shuō)明書(shū)隨貨發(fā)送,您也可以直接我司在線(xiàn)客服索取。:
中文名稱(chēng):整合素α4抗體
英文名稱(chēng):Anti-Integrin alpha 4/CD49d/Integrin α4抗體
產(chǎn)品編號(hào):BYK-0641R
別名:Alpha 4 subunit of VLA 4 receptor;ITG-α4: Antigen CD49D; CD 49d ; CD49d; CD49d antigen; CDw49d; IA4; Integrin alpha IV; ITGA 4; ITGA4; LPAM23; MGC90518; Very Late Activation Antigen 4; Very Late Activation Protein 4 Receptor Alpha 4 Subunit; VLA 4; VLA4; integrin alpha-4; ITA4_HUMAN; Integrin α4; Integrin α 4.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來(lái)源:本公司抗體有兔來(lái)源和鼠來(lái)源等
克隆類(lèi)型:兔來(lái)源為多抗,鼠來(lái)源為單抗
產(chǎn)品類(lèi)型:一抗
Anti-Integrin alpha 4抗體,整合素α4抗體產(chǎn)品介紹:Integrin alpha 4 (also called CD49d) is a 150 kDa protein that possesses a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain possessing multiple sites for phosphorylation. Integrin alpha 4 forms heterodimers with integrins beta 1 and beta 7. Integrin alpha 4 is expressed on leukocytes and leukocyte precursors, neural crest cells, and developing skeletal muscles and is essential for embryogenesis, hematopoiesis, and immune responses. The presence of integrin alpha 4 promotes cell migration and inhibits cell spreading and contractility. Integrin alpha 4 function has been implicated in the pathogenesis of multiple diseases including asthma, rheumatoid arthritis, Crohn's disease, ulcerative colitis, hepatitis C, and multiple sclerosis, and therefore, modulation of integrin alpha 4 function has become an important target for drug discovery.
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.避免反復(fù)凍融,保質(zhì)期一年。
上海研盟生物科技有限公司多抗親和層析純化
多抗的純化是一件比較簡(jiǎn)單的事情,因?yàn)槿斯じ深A(yù)比較大,所以對(duì)實(shí)驗(yàn)者的操作依賴(lài)性較強(qiáng)。很多新手覺(jué)得這是一件比較復(fù)雜困難的工作,但是如果理解了原理,就可以自行改進(jìn)實(shí)驗(yàn)了。多抗純化方法比較多,過(guò)去有用鹽析法純化的,有用辛酸-硫酸銨沉淀的方式純化的,也有用冷酒精沉淀的方式純化的,也有后來(lái)的離子交換層析和Protein A、G、A/G 純化的,但是在今天,市場(chǎng)上幾乎所有的抗體都是通過(guò)抗原親和純化得到的,與其它方法相比,抗原親和層析得到的抗體效率高、產(chǎn)量高、產(chǎn)品純、操作簡(jiǎn)單。鑒于很多網(wǎng)友問(wèn)起這方面的問(wèn)題,我就詳細(xì)地講一講親和純化原理和操作。
說(shuō)到親和層析,還得從上個(gè)世紀(jì)60年代說(shuō)起。當(dāng)年美國(guó)NIH(National Institutes of Health)
有個(gè)學(xué)生在研究一種酶和抑制劑之間的相互作用的時(shí)候,突發(fā)奇想:既然此酶可以和抑制劑結(jié)合,那如果把抑制劑固定起來(lái),不就可以用來(lái)純化酶了嗎?試了一下覺(jué)得果然可行于是發(fā)了論文,從那之后,就出現(xiàn)了各種各樣的親和層析,親和配基與配體可以是酶與底物、酶與抑制劑、激素與受體、抗原和抗體、糖蛋白與凝集素、*與親和素、*與二價(jià)金屬離子、卟啉環(huán)與亞鐵離子等。親和層析精髓:將一種分子固定,用來(lái)吸附另一種特異性分子,zui后將其洗脫下來(lái)。
Anti-Integrin alpha 4抗體,整合素α4抗體相關(guān)優(yōu)質(zhì)產(chǎn)品:
PCNA(Proliferation Cell Nuclear gen) 增殖細(xì)胞核抗原抗體
PDCD4(programmed cell death 4) 凋亡相關(guān)蛋白4抗體
PCNA(Proliferation Cell Nuclear gen) 增殖細(xì)胞核抗原抗體
TFAR19/PDCD5(TF-1 cell apoptsis-related gene 19) 凋亡相關(guān)蛋白TFAR19抗體
PCNA(Proliferation Cell Nuclear gen)human,rat,mouse,rabbit,bovine,dog and chicken 增殖細(xì)胞核抗原抗體
PCX/PODXL(Podocalyxin) 足細(xì)胞特異蛋白抗體
PDE4D(phosphodiesterase 4D) 磷酸二酯酶4D抗體
PD-1/CD279(programmed death 1) 程序性死亡1抗體
PDEF(Prostate derived Ets Factor) 上皮特異性ETs轉(zhuǎn)錄因子抗體
PDGF-A 血小板源性生長(zhǎng)因子-A抗體
PDGF-B 血小板源性生長(zhǎng)因子-B抗體
PDGF-BB(plaet derived growth factor-BB) 血小板源性生長(zhǎng)因子-BB抗體
PDGF-R-A 血小板源性生長(zhǎng)因子受體-A抗體
PDGF-R-B 血小板源性生長(zhǎng)因子受體-B抗體
PDI(Protein Disulfide lsomerase) 蛋白質(zhì)二硫鍵異構(gòu)酶抗體
Pdk4(pyruvate deYMdrogenase kinase isozyme 4) 丙酮酸脫氫酶激酶4抗體
PDX1(pancreatic and duodenal homeobox 1) 胰十二指腸同源異型盒基因抗體
PEA3(polymoma virus enhancer 3) 多瘤促活化因子抗體
PEDF (pigment epithelium-derived factor) 色素上皮源性因子抗體
PEG10(Paternally expressed gene 10) 肝癌高表達(dá)基因抗體