上海研盟生物科技有限公司Anti-Integrin alpha E抗體,整合素αE抗體Integrin αE*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取。:
中文名稱:整合素αE抗體Integrin αE
英文名稱:Anti-Integrin alpha E/CD103抗體
產(chǎn)品編號:BYK-1274R
別名:alpha-E1; alpha-M290; aM290; Antigen CD103; CD 103; CD103; CD103 antigen; HML 1 antigen; HML1 antigen; Human mucosal lymphocyte antigen 1; Human mucosal lymphocyte antigen 1, alpha subunit; Huminae; Integrin alpha E heavy chain; Integrin alpha E light chain; Integrin alpha E, epithelial-associated; Integrin alpha E1; Integrin alpha IEL; Integrin alpha M290; Integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide); Integrin, alpha E; ITGAE; MGC141996; MGC183601; Mucosal lymphocyte 1 antigen; Mucosal lymphocyte antigen 1; Integrin αE.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來源:本公司抗體有兔來源和鼠來源等
克隆類型:兔來源為多抗,鼠來源為單抗
產(chǎn)品類型:一抗
Anti-Integrin alpha E抗體,整合素αE抗體Integrin αE產(chǎn)品介紹:Integrins are heterodimers composed of noncovalently associated transmembrane a and b subunits. The 16 a and 8 b subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, Collagen and Vitronectin. Certain integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.避免反復(fù)凍融,保質(zhì)期一年。
上海研盟生物科技有限公司多抗親和層析純化
多抗的純化是一件比較簡單的事情,因?yàn)槿斯じ深A(yù)比較大,所以對實(shí)驗(yàn)者的操作依賴性較強(qiáng)。很多新手覺得這是一件比較復(fù)雜困難的工作,但是如果理解了原理,就可以自行改進(jìn)實(shí)驗(yàn)了。多抗純化方法比較多,過去有用鹽析法純化的,有用辛酸-硫酸銨沉淀的方式純化的,也有用冷酒精沉淀的方式純化的,也有后來的離子交換層析和Protein A、G、A/G 純化的,但是在今天,市場上幾乎所有的抗體都是通過抗原親和純化得到的,與其它方法相比,抗原親和層析得到的抗體效率高、產(chǎn)量高、產(chǎn)品純、操作簡單。鑒于很多網(wǎng)友問起這方面的問題,我就詳細(xì)地講一講親和純化原理和操作。
說到親和層析,還得從上個(gè)世紀(jì)60年代說起。當(dāng)年美國NIH(National Institutes of Health)
有個(gè)學(xué)生在研究一種酶和抑制劑之間的相互作用的時(shí)候,突發(fā)奇想:既然此酶可以和抑制劑結(jié)合,那如果把抑制劑固定起來,不就可以用來純化酶了嗎?試了一下覺得果然可行于是發(fā)了論文,從那之后,就出現(xiàn)了各種各樣的親和層析,親和配基與配體可以是酶與底物、酶與抑制劑、激素與受體、抗原和抗體、糖蛋白與凝集素、*與親和素、*與二價(jià)金屬離子、卟啉環(huán)與亞鐵離子等。親和層析精髓:將一種分子固定,用來吸附另一種特異性分子,zui后將其洗脫下來。
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