上海研盟生物科技有限公司Anti-Integrin Alpha V+Beta 3抗體,整合素αVβ3抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說(shuō)明書(shū)隨貨發(fā)送,您也可以直接我司在線客服索取。:
中文名稱(chēng):整合素αVβ3抗體
英文名稱(chēng):Anti-Integrin Alpha V+Beta 3(CD51+CD61)抗體
產(chǎn)品編號(hào):BYK-1310R
別名:CD51 + CD61; CD51; CD61; GP3A; GPIIIa; integrin alpha v; integrin beta 3; intregrin alpha v beta 3; ITGAV; ITGB3; Plaet membrane glycoprotein IIIa; Vitronectin receptor alpha subunit; VNRA; Antigen identified by monoclonal antibody L230; CD51 antigen; CD61 antigen; DKFZp686A08142; Integrin alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); integrin alpha v; Integrin beta 3 (plaet glycoprotein IIIa, antigen CD61); Integrin beta chain beta 3; intregrin alpha v beta 3; ITGAV; MSK8; Plaet glycoprotein IIIa; Plaet membrane glycoprotein IIIa; Vitronectin receptor alpha subunit; Vitronectin receptor subunit alpha.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來(lái)源:本公司抗體有兔來(lái)源和鼠來(lái)源等
克隆類(lèi)型:兔來(lái)源為多抗,鼠來(lái)源為單抗
產(chǎn)品類(lèi)型:一抗
Anti-Integrin Alpha V+Beta 3抗體,整合素αVβ3抗體產(chǎn)品介紹:Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. They are known to participate in cell adhesion as well as cell-surface mediated signalling. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain.
CD51 encodes integrin alpha chain V. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. The CD61 protein product is the integrin beta chain beta 3. Integrin beta 3 is found along with the alpha IIb chain in plaets.
Integrin alpha V/beta 3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha V/beta 3 recognizes the sequence R-G-D in a wide array of ligands. The alpha V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands.
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.避免反復(fù)凍融,保質(zhì)期一年。
上海研盟生物科技有限公司多抗親和層析純化
多抗的純化是一件比較簡(jiǎn)單的事情,因?yàn)槿斯じ深A(yù)比較大,所以對(duì)實(shí)驗(yàn)者的操作依賴(lài)性較強(qiáng)。很多新手覺(jué)得這是一件比較復(fù)雜困難的工作,但是如果理解了原理,就可以自行改進(jìn)實(shí)驗(yàn)了。多抗純化方法比較多,過(guò)去有用鹽析法純化的,有用辛酸-硫酸銨沉淀的方式純化的,也有用冷酒精沉淀的方式純化的,也有后來(lái)的離子交換層析和Protein A、G、A/G 純化的,但是在今天,市場(chǎng)上幾乎所有的抗體都是通過(guò)抗原親和純化得到的,與其它方法相比,抗原親和層析得到的抗體效率高、產(chǎn)量高、產(chǎn)品純、操作簡(jiǎn)單。鑒于很多網(wǎng)友問(wèn)起這方面的問(wèn)題,我就詳細(xì)地講一講親和純化原理和操作。
說(shuō)到親和層析,還得從上個(gè)世紀(jì)60年代說(shuō)起。當(dāng)年美國(guó)NIH(National Institutes of Health)
有個(gè)學(xué)生在研究一種酶和抑制劑之間的相互作用的時(shí)候,突發(fā)奇想:既然此酶可以和抑制劑結(jié)合,那如果把抑制劑固定起來(lái),不就可以用來(lái)純化酶了嗎?試了一下覺(jué)得果然可行于是發(fā)了論文,從那之后,就出現(xiàn)了各種各樣的親和層析,親和配基與配體可以是酶與底物、酶與抑制劑、激素與受體、抗原和抗體、糖蛋白與凝集素、*與親和素、*與二價(jià)金屬離子、卟啉環(huán)與亞鐵離子等。親和層析精髓:將一種分子固定,用來(lái)吸附另一種特異性分子,zui后將其洗脫下來(lái)。
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