上海研盟生物科技有限公司Anti-Integrin alpha 3抗體,整合素α3抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說(shuō)明書(shū)隨貨發(fā)送,您也可以直接我司在線客服索取。:
中文名稱:整合素α3抗體
英文名稱:Anti-Integrin alpha 3/CD49c抗體
產(chǎn)品編號(hào):BYK-2093R
別名:Integrin alpha 3 light chain; AA407068; Antigen identified by monoclonal; Integrin alpha-3; J143; CD49 antigen like family member C; CD49 antigen-like family member C; CD49c; CD49c antigen; FLJ34631; FLJ34704; FRP 2; FRP-2; FRP2; Galactoprotein B3; GAP B3; GAPB3; Integrin alpha 3; Integrin alpha-3 light chain; Integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor); ITA3_HUMAN; ITGA 3; Itga3; MSK18; VCA 2; VCA2; Very late activation protein 3 receptor alpha 3 subunit; VL3A; VLA 3 alpha chain; VLA 3 subunit alpha; VLA-3 subunit alpha; VLA3a.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來(lái)源:本公司抗體有兔來(lái)源和鼠來(lái)源等
克隆類型:兔來(lái)源為多抗,鼠來(lái)源為單抗
產(chǎn)品類型:一抗
Anti-Integrin alpha 3抗體,整合素α3抗體產(chǎn)品介紹:The protein encoded by this gene belongs to the family of integrins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain, and function as cell surface adhesion molecules. This gene encodes alpha 3 subunit, which undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 subunit to form an integrin that interacts with many extracellular-matrix proteins. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.避免反復(fù)凍融,保質(zhì)期一年。
上海研盟生物科技有限公司免疫熒光染色技術(shù)是將熒光素作為抗體標(biāo)記物進(jìn)行免疫組織化學(xué)染色,帶有熒光素的抗體孵育后可以直接使用熒光顯微鏡觀測(cè)。該技術(shù)的主要特點(diǎn)是:特異性強(qiáng)、敏感性高、速度快;缺點(diǎn)是由于熒光素有一定的淬滅周期,玻片無(wú)法*保存,需要在染色結(jié)束后盡快進(jìn)行鏡下觀測(cè)分析。
免疫熒光染色分為直接法和間接法。直接法是熒光素直接標(biāo)記在一抗上,孵育后直接觀測(cè),快速高效,還可以與多個(gè)標(biāo)記抗體一起做多重?zé)晒馊旧?。間接法是熒光素標(biāo)記在二抗上,相比直接法染色背景更干凈,費(fèi)用花費(fèi)也少。
免疫熒光染色與免疫組化前期處理相同,因?yàn)閮?nèi)源性過(guò)氧化物酶對(duì)免疫熒光實(shí)驗(yàn)沒(méi)有影響,同時(shí)雙氧水反而會(huì)干擾到熒光素發(fā)光,所以這步操作在免疫熒光中不需要做。
常用的熒光顯微鏡一般有三個(gè)通路,激發(fā)波長(zhǎng)為350nm、488nm、555nm附近,分別對(duì)應(yīng)的熒光發(fā)射光顏色為藍(lán)光、綠光、紅光。
350nm常用熒光素:DAPI(復(fù)染用),Alexa Fluor 350
488nm常用熒光素:FITC,Alexa Fluor 488
555nm常用熒光素:PE、Cy3、Rhodamine B、Alexa Fluor555
另有其他常見(jiàn)熒光素
594nm常用熒光素:Texas Red、Alexa Fluor594
647nm常用熒光素:Cy5、APC、Alexa Fluor647
免疫熒光雙重染色熒光雙染或多重染色是指將帶有不同熒光素標(biāo)記的抗體孵育在同一張切片上,鏡下觀察時(shí)將不同的顏色組合在一起,將不同的抗原表達(dá)情況一起呈現(xiàn)出來(lái),這樣分析多個(gè)抗原的關(guān)聯(lián)性更客觀、更直接。
熒光雙染也有直接法和間接法。直接法是使用帶有不同熒光素標(biāo)記的兩個(gè)抗體同時(shí)孵育后觀測(cè);間接法需要用到兩個(gè)不同種屬來(lái)源的一抗(例如一個(gè)鼠源抗體,一個(gè)兔源抗體),后面要選擇與一抗相對(duì)應(yīng)的二抗同時(shí)需要帶有不同的熒光素標(biāo)記(例如FITC標(biāo)記山羊抗小鼠和Cy3標(biāo)記山羊抗兔),間接法需要同時(shí)兼顧一抗和二抗的種屬關(guān)系,避免出現(xiàn)相互的交叉反應(yīng)。
Anti-Integrin alpha 3抗體,整合素α3抗體相關(guān)優(yōu)質(zhì)產(chǎn)品:
SHP2/PTPN11(Src homology 2 (SH2) domain containing phosphotyrosinephosphatase 2) 蛋白*磷酸酶2抗體
SIP1 (survival of motor neuron protein interacting protein 1) 運(yùn)動(dòng)神經(jīng)元存活蛋白結(jié)合蛋白1抗體
SIRT1(sirtuin 1) 沉默調(diào)節(jié)蛋白1抗體
Skp2 (S-phase kinase-associated protein 2) 細(xì)胞S相激酶相關(guān)蛋白抗體
SLC4A4(solute carrier family 4:sodium bicarbonate cotransporter NBC1) *協(xié)同轉(zhuǎn)運(yùn)蛋白4-A4抗體
Slc4a7/Nbcn1(solute carrier family 4, sodium bicarbonate cotransporter, member 7) *協(xié)同轉(zhuǎn)運(yùn)蛋白4-A7抗體
Slc22A17 (solute carrier famili 22A17) 可溶性載質(zhì)轉(zhuǎn)運(yùn)蛋白22A17抗體
SLC24A5 (solute carrier family 24, member 5) 可溶性載質(zhì)轉(zhuǎn)運(yùn)蛋白SLC24A5抗體
Slc26a5/Prestin 外毛細(xì)胞運(yùn)動(dòng)蛋白/可溶性載質(zhì)轉(zhuǎn)運(yùn)蛋白Slc26a5抗體
SLC6A19 (Solute carrier family 6, member 19) 鉀依賴鈉鈣交換蛋白6
Smac/DIABLO(the second mitochondrial activator of caspase) 線粒體促凋亡蛋白抗體
SLC33A1(Acetyl-coenzyme A transportor 1) 乙酰*轉(zhuǎn)運(yùn)蛋白1抗體
SLC34A2/NaPi-2b(Solute carrier family 34 member 2) 磷酸鈉協(xié)同轉(zhuǎn)運(yùn)蛋白抗體