phospho-CRK (Tyr221)抗體-磷酸化Crk p38抗體抗原修復(fù)的幾種常用方法(供參考)
1、 微波爐加熱:
在微波爐里加熱0.01*緩沖液(pH6.0)至沸騰后將組織芯片放入,斷電,間隔5-10分鐘,反復(fù)1-2次。根據(jù)玻片情況可參考采用微波加熱“3-5-3法”3分鐘溫火沸騰后靜止5分鐘,再溫火沸騰3分鐘即可。
適用的抗原:來(lái)源于人、大鼠、小鼠的組織標(biāo)本;來(lái)源于其他動(dòng)物種屬的組織標(biāo)本。
2、 沸熱修復(fù):
電爐或水浴鍋加熱0.01M*緩沖液(pH6.0)至95℃左右,放入組織芯片加熱10-15分鐘。
3、 高壓熱修復(fù):
在沸水中加入EDTA(pH8.0)或0.01m*緩沖溶液(pH6.0).蓋上不銹鋼鍋蓋,但不能鎖定。將玻片置于金屬染色架上,緩慢加壓,使玻片在緩沖液中浸泡五分鐘,然后將蓋子鎖定,小閥門將會(huì)升起來(lái)。10分鐘后除去熱源,置入涼水中,當(dāng)小閥門沉下去后打開(kāi)蓋子。此方法適用于較難檢測(cè)或核抗原的抗原修復(fù)。(骨及軟骨組織的標(biāo)本選擇較溫和的微波加熱修復(fù)) 。
4、 酶消化方法:
常用0.1%*和0.4%*液。*使用前預(yù)熱37℃,消化時(shí)間約為5-30分鐘。
適用于被固定遮蔽的抗原:包括 Collagen.GFAP. Complement.Cytokeratin.c-erB-2.LCA.LN.等。
【中文名稱】:磷酸化Crk p38抗體
【英文名稱】:Anti-phospho-CRK (Tyr221)
【產(chǎn)品編號(hào)】:BYK-3121R
【規(guī) 格】:0.1ml/0.2ml
【相關(guān)標(biāo)記】:Alexa Fluor 350、Alexa Fluor 488、Alexa Fluor 555、Alexa Fluor 647、AP、APC、Biotin、Cy3、Cy5、Cy5.5、Cy7、FITC、Gold、HRP、PE、PE-Cy3、PE-CY5、PE-CY5.5、PE-CY7、RBITC.
【濃 度】:1mg/1ml
【抗體來(lái)源】:Rabbit or Mouse or Goat
【克隆類型】:polyclonal or monoclonal
【產(chǎn)品類型】:一抗
【產(chǎn)品應(yīng)用】:可以用于做石蠟切片免疫組化,冰凍切片免疫組化,Elisa,WB,免疫熒光、流式細(xì)胞術(shù)等實(shí)驗(yàn)。
抗體修復(fù)方式
修復(fù)液:0.01M 枸櫞酸(pH6.0)或者0.05M EDTA(pH8.0)
phospho-CRK (Tyr221)抗體-磷酸化Crk p38抗體產(chǎn)品介紹:Crk p38 (CrkII) is an isoform of the protein Crk. CrkI and CrkII are produced from the same crk gene by alternative splicing. The two isoforms differ in their biological activities with CrkII having less transforming activity than CrkI. Crk p38 is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. It has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Crk is believed to be a regulator of invasive responses because increased levels of the protein have been observed in multiple human cancers. In vivo studies have demonstrated that decreased levels of Crk remarkably inhibits tumor formation and its invasive growth.
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phospho-CRK (Tyr221)抗體-磷酸化Crk p38抗體英文別名:Crk p38 (phospho Y221); p-Crk p38 (phospho Y221); p-CRK(phospho-Tyr221); p-CRK(phospho-Y221); Crk p38; CRK CRK isoform 2; CRK isoform II; CRKII; p38; Proto oncogene C crk; v crk avian sarcoma virus CT10 oncogene homolog ; v crk sarcoma virus CT10 oncogene homolog; v crk sarcoma virus CT10 oncogene homolog (avian); Adapter molecule crk; Avian sarcoma virus CT10 (v crk) oncogene homolog; CRK_HUMAN