上海研盟生物科技有限公司RanBP3抗體-RAN結(jié)合蛋白3抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細胞術(shù)等實驗中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?/p>
簡單介紹:
中文名稱:RAN結(jié)合蛋白3抗體
英文名稱:Anti-RanBP3
產(chǎn)品編號:BYK-9334R
產(chǎn)品別名:KIAA1499; NPL4; NPL4, S. cerevisiae, homolog of; NPL4_HUMAN; nploc4; nuclear protein localization 4 homolog (S. cerevisiae); Nuclear protein localization 4 homolog; Nuclear protein localization protein 4 homolog; Nuclear protein localization protein 4, S. cerevisiae, homolog of; Protein NPL4.
產(chǎn)品規(guī)格:0.1ml、0.2ml、1ml
產(chǎn)品用途:科研實驗
產(chǎn)品價格:請報價
說明書:請?zhí)砑涌头苯铀魅?/p>
抗體來源:該指標(biāo)有兩種產(chǎn)品,一種是兔來源抗體,一種是鼠來源抗體
克隆類型:兔來源為多克隆抗體,鼠來源單克隆抗體
交叉反應(yīng):請索取說明書查看
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
RanBP3抗體-RAN結(jié)合蛋白3抗體產(chǎn)品介紹:The NPL4 gene encodes the 608-amino acid, endoplasmic reticulum and nuclear membrane protein Npl4 that forms a complex with p97/Cdc48p and Ufd1p. This complex plays a role in IP3 receptor processing by recognizing ubiquitinated IP3 receptors in the endoplasmic reticulum and delivering them to the proteasome for degradation. The Npl4 protein contains 8 potential N-myristoylation sites, 5 potential N-glycosylation sites, several phosphorylation sites, and a C-terminal zinc finger motif. This protein is 96% homologous to the rat Npl4 protein, 44% homologous to the Caenorhabditis elegans Npl4 protein, and 34% homologous to the Saccharomyces cerevisiae Npl4 protein. Mutations in the NPL4 gene cause defects in nuclear envelope morphology, nuclear protein import, and nuclear poly(A) RNA export.
多肽的溶解與保存
一、凍干多肽的溶解
1、多肽溶解的主要問題是二級結(jié)構(gòu)的形成。雖然疏水肽鏈二級結(jié)構(gòu)的形成更明顯,但除zui短的肽鏈外,此現(xiàn)象產(chǎn)生在幾乎所有肽鏈,與極性無關(guān)。所以,溶解多肽的*個原則是使用無菌蒸餾水或去離子水,當(dāng)然無氧水。多肽溶液可能遇到細菌降解,為防止此情況的發(fā)生,應(yīng)溶解在無菌的蒸餾水中或用0.45或0.2孔徑的濾膜過濾除菌。含有Cys、Met、Trp的多肽很容易氧化,應(yīng)溶于無氧的水中,無氧水可通過注入惰性氣體(氮、氦、氬)減壓除氣得到;
2、若多肽不溶于純水,超聲處理有助于打碎顆粒并增加溶解度。注意:超聲處理會引起溶液發(fā)熱或多肽降解;
3、若多肽含有多個堿性氨基酸,使用(1-10%)的乙酸水溶液:對于疏水性強的多肽,應(yīng)使用50%的乙酸;
4、若多肽中含有大量酸性氨基酸,可用氨溶液(1-10%)或乙酸乙基*或重碳酸鹽等揮發(fā)性的堿性緩沖液溶解。pH值在層析前必須調(diào)整;
5、異丙醇和乙腈能溶解中等大小的多肽。若肽要上柱,有機溶劑的量必須很少否則將嚴(yán)重影響停留時間;
6、若多肽因含有Val、Leu、Met、Phe、Tyr、Ala等芳香鏈而高度疏水、或為中性肽時,DMF或DMSO等膜變性劑的使用,有利于多肽的溶解:
a.高濃度模變性劑通過破壞多肽的二級結(jié)構(gòu)而助溶;
b.膜變性劑適于多肽分析液的制備,但可能會對其生物活性的研究工作造成干擾;
c.DMF是變性劑(zui高濃度可達30%),滴加至多肽溶解;
d.反相層析法時,DMF將與洗脫液前鋒一起流出,根據(jù)入量的多少,峰值可能很高。大多數(shù)肽能在大量DMF流出后幾分鐘內(nèi)流出,若肽鏈很小,洗脫太早,多肽的量將很低。
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