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相關標記抗體:HRP標記抗體,Biotin標記抗體,Gold標記抗體,RBITC標記抗體,AP標記抗體,FITC標記抗體,Cy3標記抗體,Cy5標記抗體,Cy5.5標記抗體,Cy7標記抗體,PE標記抗體,PE-Cy3標記抗體,PE-Cy5標記抗體,PE-Cy5.5標記抗體,PE-Cy7標記抗體,APC標記抗體,Alexa Fluor 350標記抗體,Alexa Fluor 488標記抗體,Alexa Fluor 555標記抗體,Alexa Fluor 647標記抗體
上海研盟生物科技有限公司Anti-PTCHD1抗體*,主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?/span>
英文名稱:Anti-PTCHD1 antibody
中文名稱:PTCHD1蛋白抗體
PTCHD1蛋白抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
★研盟生物★PTCHD1蛋白抗體,Anti-PTCHD1抗體
免疫組化操作步驟
(一)、儀器設備
1、 18cm不銹鋼高壓鍋或電爐或用微波爐
2、 水浴鍋
(二)、試 劑
1、 PBS緩沖液(pH7.2―7.4)
2、 0.01mol/L檸檬酸鈉緩沖液(CB,pH6.0,1000ml)
3、 3%甲醇―H2O2溶液:用30% H2O2 和80%甲醇溶液配制
4、 風裱劑:
a. 甘油和0.5mmol/L碳酸鹽緩沖液(pH9.0–9.5)等量混合
b. 油和TBS(PBS)配制
5、 TBS/PBS pH9.0–9.5,適用于熒光纖維鏡標本;pH7.0-7.4適合光學纖維標本
(三)、操作流程
1、 脫蠟和水化:脫蠟前應將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘
a. 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b. 無水乙醇中浸泡五分鐘
c. 95%乙醇中浸泡五分鐘
d. 75%乙醇中浸泡五分鐘
2、 抗原修復:用于福爾馬林固定的石蠟包埋組織芯片
PTCHD1蛋白抗體,Anti-PTCHD1抗體溶解方法:
方法1:我們的抗體(凍干粉)已經(jīng)包含了0.01M PBS、1% BSA和0.1% 防腐劑(疊氮鈉或*),您只需要加入滅菌的雙蒸水就行了。抗體溶解后,置于-20℃保存,分裝后使用,避免反復凍融,可保證至少1年有效;
方法2:也可以只加入一半體積的雙蒸水,再用一半體積的甘油補足,置于-20℃保存,這樣抗體不會凍,有利于抗體的穩(wěn)定。
后續(xù)試驗時,再使用對應的緩沖液稀釋成工作液,即用即配。
我們的抗體(凍干粉),在常溫放置下,至少一個月有效;若在-20℃下保存(*),至少三年有效。
Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:
1) Reconstituting by directly adding 100uL of sterile, distilled water.
2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.
簡單介紹:
產(chǎn)品別名:9630036J22Rik; AI848149; FLJ30296; Gm387; MGC149798; Patched domain containing 1; Patched domain-containing protein 1; PTCHD1; PTHD1_HUMAN; RGD1564527; RP23-152D15.3.
抗體來源:Rabbit or Mouse
保質(zhì)期:1年
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:This gene encodes a membrane protein with a patched domain. The encoded protein is similar to Drosophila proteins which act as receptors for the morphogen sonic hedgehog. Deletions in this gene, which is located on the X chromosome, are associated with inlectual disability and autism (PMID: 21091464, PMID: 20844286).
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Smad4(MotheBYK against decapentaplegic homolog 4) Smad4抗體
Smad7(MotheBYK against decapentaplegic homolog 7) Smad 7抗體
phosphor-SMAD(p-Ser425)、phosphor-MotheBYK against decapentaplegic 磷酸化SMAD抗體
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Regucalcin/SMP30/Rgn(Senescence marker protein 30) 衰老標記蛋白30抗體
Snake poison Protein 抗蛇毒蛋白抗體(蝮蛇)
Snake poison Protein 抗蛇毒蛋白抗體(中華眼鏡蛇)
SNAP-25 (synaptosomal-associated protein 25) 突觸相關蛋白25抗體
Snail 抗Snail蛋白抗體
Socs 1 (suppressor of cytokine signaling 1) 細胞因子信號傳導抑制蛋白1抗體
Socs 2(suppressor of cytokine signaling 2) 細胞因子信號傳導抑制蛋白2抗體
Socs 3 (suppressor of cytokine signaling 3) 細胞因子信號傳導抑制蛋白3抗體