人Ⅰ類主要組織兼容性復(fù)合體E檢測試劑盒定量檢測,具有靈敏性高,特異性強,重復(fù)性好,性價比高,線性好;操作簡單,精確度高;售前售后完善,隨時來實驗室現(xiàn)場觀看,常見ELISA試劑盒系列:人,大鼠,小鼠:TNF-α、IL-1β、IL-7、IL-32、IL-21、IL-27、IL-23、IFN-γ、TGF-α、TGF-β1等。
英文簡稱:Human Major Histocompatibility Complex Class I E (MHCE) ELISA Kit
人Ⅰ類主要組織兼容性復(fù)合體E檢測試劑盒注意事項以下四點:
1,封閉:以下包以后是無關(guān)的蛋白質(zhì)溶液濃度高,涂層技術(shù)。隨函附上使許多不相關(guān)的蛋白質(zhì)充填這些空隙,和攪擾物質(zhì)的免疫排擠和吸附進程。ELISA試劑盒常用的密封:0。05% - 0。5%牛血清白蛋白;10%或1%明膠牛血清;脫脂奶粉,報價相對低廉,可用于高濃度(5%~10%);和一些稀有的運用各種動物血清(首要是為了消除類似的蛋白和酪蛋白等攪擾)。但究竟挑選啥,依據(jù)試驗的詳細(xì)實習(xí)。
2,洗板:能夠說,在中操作,清洗是在首要的關(guān)鍵技術(shù)。因為聚苯乙烯等塑料對蛋白質(zhì)的吸附是普遍存在的,為了完成自在酶與客觀相的符號的別離,在板料孔中殘留游離和吸附的非特異性攪擾物質(zhì)的去掉,洗刷,并應(yīng)將攪擾物質(zhì)洗刷下來的非特異性吸附。所以在洗衣板會有必定的誤差,非常人的要素(當(dāng)然也有洗衣機除條件),是不完整的或弦清孔,體系的影響是如此靈敏,但不小。
3,加抗體(抗標(biāo)本和兩個):留意的槍頭,槍頭。樣品用稀釋稀釋,也能夠用稀釋的閉解。假如要增加兩個電阻,但也要留意兩個反作業(yè)濃度的廢物,太高,太低的光的色彩。
4,色彩:色彩體系有許多,咱們開端做,挑選恰當(dāng)?shù)纳鼠w系。ELISA試劑盒留意色彩體系酶底物物加硫柳泵節(jié)省,組合物加*。7,每次都盡可能的陰和陽空三操控,如剖析疑問的呈現(xiàn)。YB-PECAM1-Mu 小鼠血小板內(nèi)皮細(xì)胞粘附分子1(PECAM1) ELISA Kit Mouse Plaet/Endothelial Cell Adhesion Molecule (PECAM1) ELISA Kit Mus musculus mouse 15.625-1000pg/mL 6.5pg/mL sandwich 96T/48T 905/633.5 12 months
YB-PECAM1-Ra 大鼠血小板內(nèi)皮細(xì)胞粘附分子1(PECAM1) ELISA Kit Rat Plaet/Endothelial Cell Adhesion Molecule (PECAM1) ELISA Kit Rattus norvegicus Rat 31.25-2000pg/mL 9.5pg/mL sandwich 96T/48T 950/665 12 months
YB-PEDF-b 牛色素上皮衍生因子(PEDF) ELISA Kit Bovine Pigment Epithelium Derived Factor (PEDF) ELISA Kit Bos taurus; Bovine Cattle 0.312-20ng/mL 0.135ng/mL sandwich 96T/48T 1092.5/764.7 12 months
YB-PEDF-Hu 人色素上皮衍生因子(PEDF) ELISA Kit Human Pigment Epithelium Derived Factor (PEDF) ELISA Kit Homo sapiens human 1.875-120ng/mL 0.76ng/mL sandwich 96T/48T 926.3/648.5 12 months
YB-PEDF-Mu 小鼠色素上皮衍生因子(PEDF) ELISA Kit Mouse Pigment Epithelium Derived Factor (PEDF) ELISA Kit Mus musculus mouse 0.781-50ng/mL 0.35ng/mL sandwich 96T/48T 950/665 12 months
YB-PEDF-Ra 大鼠色素上皮衍生因子(PEDF) ELISA Kit Rat Pigment Epithelium Derived Factor (PEDF) ELISA Kit Rattus norvegicus Rat 1.56-100ng/mL 0.55ng/mL sandwich 96T/48T 997.5/698.3 12 months
YB-PENK-Hu 人前腦啡肽(PENK) ELISA Kit Human Proenkephalin (PENK) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.062ng/mL sandwich 96T/48T 970.1/679.1 12 months
YB-PENK-Mu 小鼠前腦啡肽(PENK) ELISA Kit Mouse Proenkephalin (PENK) ELISA Kit Mus musculus mouse 78.125-5000pg/mL 21.3pg/mL sandwich 96T/48T 995/696.5 12 months
YB-PEPD-Hu 人肽酶D(PEPD) ELISA Kit Human Peptidase D (PEPD) ELISA Kit Homo sapiens human 1.56-100ng/mL 0.63ng/mL sandwich 96T/48T 1057.5/740.3 12 months
YB-PER1-Hu 人節(jié)律晝夜蛋白1(PER1) ELISA Kit Human Period Circadian Protein 1 (PER1) ELISA Kit Homo sapiens human 0.312-20ng/mL 0.114ng/mL sandwich 96T/48T 1057.5/740.3 12 months
YB-PER2-Ra 大鼠節(jié)律晝夜蛋白2(PER2) ELISA Kit Rat Period Circadian Protein 2 (PER2) ELISA Kit Rattus norvegicus Rat 0.312-20ng/mL 0.101ng/mL sandwich 96T/48T 1140/798 12 months
YB-PEX2-Hu 人過氧化物酶體生物合成因子2(PEX2) ELISA Kit