人Ⅰ類主要組織兼容性復合體C檢測試劑盒定量檢測,具有靈敏性高,特異性強,重復性好,性價比高,線性好;操作簡單,精確度高;售前售后完善,隨時來實驗室現(xiàn)場觀看,常見ELISA試劑盒系列:人,大鼠,小鼠:TNF-α、IL-1β、IL-7、IL-32、IL-21、IL-27、IL-23、IFN-γ、TGF-α、TGF-β1等。
英文簡稱:Human Major Histocompatibility Complex Class I C (MHCC) ELISA Kit
人Ⅰ類主要組織兼容性復合體C檢測試劑盒注意事項以下四點:
1,封閉:以下包以后是無關的蛋白質(zhì)溶液濃度高,涂層技術。隨函附上使許多不相關的蛋白質(zhì)充填這些空隙,和攪擾物質(zhì)的免疫排擠和吸附進程。ELISA試劑盒常用的密封:0。05% - 0。5%牛血清白蛋白;10%或1%明膠牛血清;脫脂奶粉,報價相對低廉,可用于高濃度(5%~10%);和一些稀有的運用各種動物血清(首要是為了消除類似的蛋白和酪蛋白等攪擾)。但究竟挑選啥,依據(jù)試驗的詳細實習。
2,洗板:能夠說,在中操作,清洗是在首要的關鍵技術。因為聚苯乙烯等塑料對蛋白質(zhì)的吸附是普遍存在的,為了完成自在酶與客觀相的符號的別離,在板料孔中殘留游離和吸附的非特異性攪擾物質(zhì)的去掉,洗刷,并應將攪擾物質(zhì)洗刷下來的非特異性吸附。所以在洗衣板會有必定的誤差,非常人的要素(當然也有洗衣機除條件),是不完整的或弦清孔,體系的影響是如此靈敏,但不小。
3,加抗體(抗標本和兩個):留意的槍頭,槍頭。樣品用稀釋稀釋,也能夠用稀釋的閉解。假如要增加兩個電阻,但也要留意兩個反作業(yè)濃度的廢物,太高,太低的光的色彩。
4,色彩:色彩體系有許多,咱們開端做,挑選恰當?shù)纳鼠w系。ELISA試劑盒留意色彩體系酶底物物加硫柳泵節(jié)省,組合物加*。7,每次都盡可能的陰和陽空三操控,如剖析疑問的呈現(xiàn)。YB-PEX2-Mu 小鼠過氧化物酶體生物合成因子2(PEX2) ELISA Kit Mouse Peroxisomal Biogenesis Factor 2 (PEX2) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.05ng/mL sandwich 96T/48T 905/633.5 12 months
YB-PEX2-Ra 大鼠過氧化物酶體生物合成因子2(PEX2) ELISA Kit Rat Peroxisomal Biogenesis Factor 2 (PEX2) ELISA Kit Rattus norvegicus Rat 0.156-10ng/mL 0.08ng/mL sandwich 96T/48T 950/665 12 months
YB-PF4-b 牛血小板因子4(PF4) ELISA Kit Bovine Plaet Factor 4 (PF4) ELISA Kit Bos taurus; Bovine Cattle 0.781-50ng/mL 0.27ng/mL sandwich 96T/48T 1040/728 12 months
YB-PF4-g 山羊血小板因子4(PF4) ELISA Kit Goat Plaet Factor 4 (PF4) ELISA Kit Capra hircus; Caprine Goat 3.125-200ng/mL 0.75ng/mL sandwich 96T/48T 1062.5/743.7 12 months
YB-PF4-Hu 人血小板因子4(PF4) ELISA Kit Human Plaet Factor 4 (PF4) ELISA Kit Homo sapiens human 1.56-100pg/mL 0.58pg/mL sandwich 96T/48T 795/556.5 12 months
YB-PF4-Mu 小鼠血小板因子4(PF4) ELISA Kit Mouse Plaet Factor 4 (PF4) ELISA Kit Mus musculus mouse 78.125-5000pg/mL 27pg/mL sandwich 96T/48T 667.5/467.3 12 months
YB-PF4-p 豬血小板因子4(PF4) ELISA Kit Porcine Plaet Factor 4 (PF4) ELISA Kit Sus scrofa; Porcine Pig 0.312-20ng/mL 0.151ng/m sandwich 96T/48T 1040/728 12 months
YB-PF4-Ra 大鼠血小板因子4(PF4) ELISA Kit Rat Plaet Factor 4 (PF4) ELISA Kit Rattus norvegicus Rat 0.312-20ng/mL 0.128ng/mL sandwich 96T/48T 950/665 12 months
YB-PF4-Rb 兔血小板因子4(PF4) ELISA Kit Rabbit Plaet Factor 4 (PF4) ELISA Kit Oryctolagus cuniculus Rabbit 0.156-10ng/mL 0.054ng/mL sandwich 96T/48T 950/665 12 months
YB-PF4V1-Hu 人血小板因子4V1(PF4V1) ELISA Kit Human Plaet Factor 4 Variant 1 (PF4V1) ELISA Kit Homo sapiens human 1.56-100ng/mL 0.8ng/mL sandwich 96T/48T 926.3/648.5 12 months
YB-PFDN1-Hu 人前折疊蛋白亞基1(PFDN1) ELISA Kit Human Prefoldin Subunit 1 (PFDN1) ELISA Kit Homo sapiens human 0.312-20ng/mL 0.112ng/mL sandwich 96T/48T 970.1/679.1 12 months
YB-PFKL-Ra 大鼠肝臟磷酸果糖激酶(PFKL) ELISA Kit Rat Phosphofructokinase, Liver (PFKL) ELISA Kit Rattus norvegicus Rat 1.56-100ng/mL 0.58ng/mL sandwich 96T/48T 1045.1/731.6 12 months
YB-PFKM-Hu 人肌肉磷酸果糖激酶(PFKM) ELISA Kit Human Phosphofructokinase, Muscle (PFKM) ELISA Kit Homo sapiens human 0.312-20ng/mL 0.114ng/mL sandwich 96T/48T 970.1/679.1 12 months
YB-PFKM-Ra 大鼠肌肉磷酸果糖激酶(PFKM) ELISA Kit Rat Phosphofructokinase, Muscle (PFKM) ELISA Kit Rattus norvegicus Rat 0.312-20ng/mL 0.127ng/mL sandwich 96T/48T 1045.1/731.6 12 months
YB-PFKP-Hu 人血小板型磷酸果糖激酶(PFKP) ELISA K