Thermo Scientific RevertAid鏈cDNA合成試劑盒包含了足夠進(jìn)行100次x20 µL反應(yīng)的試劑。本品是以RNA為模板合成鏈cDNA的完整系統(tǒng),可合成長(zhǎng)達(dá)13kb的cDNA。本品含有RiboLock RNase抑制劑,能夠有效保護(hù)RNA模板不受降解。
Thermo Scientific RevertAid鏈cDNA合成試劑盒配套提供oligo(dT)18引物和隨機(jī)六聚體引物。oligo(dT)18引物可以選擇性地與mRNA中的poly (A)尾結(jié)合。隨機(jī)六聚體引物無(wú)需poly (A)尾,因此可轉(zhuǎn)錄mRNA 5’-端區(qū)域,也可以無(wú)poly (A)尾的RNA (如micoRNA)為模板合成cDNA。該試劑盒也可使用基因特異性引物。
應(yīng)用鏈cDNA合成,作為RT-PCR和實(shí)時(shí)RT-qPCR的模板
構(gòu)建全長(zhǎng)cDNA文庫(kù)
反義RNA合成
Full-length cDNA synthesis using the RevertAid First Strand cDNA Synthesis Kit
適用于(應(yīng)用) | Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR) |
Optimal Reaction Temperature: | 42° C |
Product Line | RevertAid |
Reaction Format | Separate Components |
Reverse Transcriptase | RevertAid |
樣品類(lèi)型 | RNA |
足夠用于 | 100 reactions |
- ?The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Oligo(dT)18 Primer, Random Hexamer Primer, Control GAPDH RNA, 10 μM Forward GAPDH Primer, 10 μM Reverse GAPDH Primer, and nuclease-free water.
- ?Store at -20°C.