隨著植物科學(xué)的發(fā)展,越來(lái)越多的研究人員需要在活體植株中驗(yàn)證基因的功能,研究基因的表達(dá)調(diào)控,以及植物的晝夜紀(jì)律等,因此,對(duì)于植物活體成像的需求也越來(lái)越大。植物活體植物成像或植物組織成像,還可以應(yīng)用于植物陽(yáng)性克隆的篩選以及轉(zhuǎn)基因植物的鑒定等多方面。
Vilber自推出小動(dòng)物活體成像后,近日再次推出專門應(yīng)用于植物活體或組織的成像系統(tǒng)——NEWTON7.0 Bio
基于NEWTON7.0 平臺(tái),NEWTON7.0 Bio具有超高的檢測(cè)靈敏度以及靈活的操控性。
— 多通道熒光光源可應(yīng)用于不同染料標(biāo)記或報(bào)告基因的檢測(cè),如植物研究中常見的GFP,RFP等;
— 深度制冷的CCD相機(jī),可長(zhǎng)時(shí)間曝光,尤其適用于Luc報(bào)告基因的檢測(cè),避免了葉綠素等自發(fā)熒光的干擾;
— X,Y,Z軸三維移動(dòng)的載物臺(tái),可根據(jù)植物樣品進(jìn)行快速對(duì)焦,適用于培養(yǎng)皿中的幼苗,植物葉片,植物種子,小型植株等;
— 可調(diào)角度的載物臺(tái),避免了植物葉片間的遮擋,便于根據(jù)需求針對(duì)特定部位進(jìn)行成像;
應(yīng)用實(shí)例
植物逆境脅迫&基因表達(dá)調(diào)控
Analysis of CtHsfA2bbinding to ProAtApx2 in vivo with using luciferase(LUC) assay in Arabidopsisprotoplast.Signs of +/- indicates with/without the components listed on theleft side.
Wang, X. et al. Transcriptional regulation ofheat shock proteins and ascorbate peroxidase by CtHsfA2b from Africanbermudagrass conferring heat tolerance in Arabidopsis.Sci. Rep.6,28021;doi:10.1038/srep28021(2016)
植物克隆篩選
Arabidopsis thaliana seedlings transfected with luciferase (right) and non-transfected (left), 3min exposure after 1 mM luciferin was sprayed onto the leaves.
植物微生物侵染研究
BIK1 and FLS2 interact with RbohD in N. benthamiana. The indicated constructs were transiently expressed in N. benthamiana, and luciferase complementation imaging assay was performed.
轉(zhuǎn)基因植物鑒定
GFP expression, GFP-transfected (right) and Control (left) grain of rice and leaves, Epi excitation 470nm and emission filter f565, exposure time 2sec.
NEWTON7.0 Bio為植物領(lǐng)域科研工作者提供了更多的選擇,植物(植株/幼苗/種子)成像也將會(huì)為研究結(jié)果的最終驗(yàn)證提供力的支持!