CORTECS UPLC 1.6 µm實(shí)心核顆粒色譜柱是業(yè)內(nèi)性能。當(dāng)與低擴(kuò)散體積的UPLC系統(tǒng)使用時(shí)，它能夠提供別的柱效。與粒徑相當(dāng)?shù)娜嗫滋盍舷啾龋?/span>CORTECS UPLC 1.6 µm實(shí)心核顆粒柱可以給出更尖銳、更窄的色譜峰。到現(xiàn)在我們已經(jīng)發(fā)展出7種的CORTECS色譜柱鍵合相，包括從反相到HILIC柱，以便分離性質(zhì)不同的各種分析物。
與相似粒徑的全多孔顆粒相比，實(shí)心核顆粒因其形態(tài)特征的特殊性而更有可能獲得更高柱效。要實(shí)現(xiàn)這個(gè)理論柱效，還需要配合正確的色譜柱硬件和的柱裝填技術(shù)。沃特世憑借多年的UPLC柱生產(chǎn)經(jīng)驗(yàn)，采用超低擴(kuò)散的色譜柱硬件與柱裝填技術(shù)，配合全新CORTECS顆粒，樹(shù)立了UPLC色譜柱柱效新標(biāo)準(zhǔn)。
基于2010年沃特世成功推出的表面帶電雜化顆粒技術(shù)(CSH™)，為顆粒表面賦予少量正電荷，可以使用低離子強(qiáng)度的流動(dòng)相(如甲酸)，對(duì)堿性化合物以及肽仍能獲得的峰形與載量。
采用CORTECS HILIC色譜柱改善極性分析物的保留性能，親水作用色譜(HILIC)是一種對(duì)在反相色譜中保留性能較差的強(qiáng)極性分析物進(jìn)行有效保留的模式。CORTECS HILIC色譜柱所含的未鍵合實(shí)心顆?？梢员Ａ魳O性化合物。在反相色譜中，通常采用高比例水相流動(dòng)相來(lái)保留極性分析物，HILIC則是使用含有高比例有機(jī)溶劑的流動(dòng)相實(shí)現(xiàn)對(duì)極性分析物的保留。
利用CORTECS UPLC色譜柱提高樣品通量
背景：在液相色譜分析中，如果樣品運(yùn)行過(guò)程 十分耗時(shí)，那么會(huì)由于對(duì)寶貴儀器的長(zhǎng) 時(shí)間占用和處理樣品數(shù)目的限制，導(dǎo)致 實(shí)驗(yàn)室整體工作效率降低。通過(guò)縮短樣 品分析時(shí)間來(lái)提高樣品通量是很困難的， 因?yàn)槌３Ｐ枰獱奚蛛x度或靈敏度等重 要色譜屬性。然而，采用CORTECS UPLC色譜柱就可以在保持峰容量的同時(shí)提高樣品通量。
解決方案：高效的CORTECS UPLC色譜柱采用了的 實(shí)心核顆粒和填充技術(shù)，能夠在高流速 下保持良好的分離性能。這讓色譜工作 者可以根據(jù)需要提高方法的流速，進(jìn)而縮短運(yùn)行時(shí)間并提高通量，而且無(wú)需犧 牲分離質(zhì)量。圖1所示為使用 CORTECS UPLC C18 +色譜柱分析磺胺類(lèi)藥物，并使用 全多孔C18色譜柱進(jìn)行初始分離的結(jié)果比 較。CORTECS UPLC C18+色譜柱流速為初始 分離的兩倍，并按照流速變化對(duì)梯度作 出相應(yīng)調(diào)整。
品名 | 編號(hào) |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 2.1 mm X 150 mm, 3/pkg | 176003931 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 100 mm, 3/pkg | 186008699 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 3 mm X 30 mm, 3/pkg | 176003932 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 75 mm, 3/pkg | 186008698 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 3 mm X 50 mm, 3/pkg | 176003933 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 3 mm X 75 mm, 3/pkg | 176003934 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 2.1 mm X 150 mm, 1/pkg | 186008695 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 2.1 mm X 100 mm, 1/pkg | 186008694 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 50 mm, 3/pkg | 186008697 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 30 mm, 3/pkg | 186008696 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 2.1 mm X 100 mm, 3/pkg | 176003930 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 2.1 mm X 30 mm, 1/pkg | 186008691 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 2.1 mm X 75 mm, 1/pkg | 186008693 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 2.1 mm X 50 mm, 1/pkg | 186008692 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 150 mm, 3/pkg | 186008700 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 3 mm X 30 mm, 1/pkg | 186008701 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 3 mm X 150 mm, 1/pkg | 186008705 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 3 mm X 100 mm, 1/pkg | 186008704 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 3 mm X 75 mm, 1/pkg | 186008703 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 3 mm X 50 mm, 1/pkg | 186008702 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 2.1 mm X 75 mm, 3/pkg | 176003929 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 2.1 mm X 50 mm, 3/pkg | 176003928 |
CORTECS T3 VanGuard Cartridge, 120?, 2.7 µm, 3.9 mm X 5 mm, 3/pkg | 186008507 |
CORTECS T3 VanGuard Pre-column, 120?, 1.6 µm, 2.1 mm X 5 mm, 3/pkg | 186008508 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 2.1 mm X 30 mm, 3/pkg | 176003927 |
CORTECS UPLC T3 Column, 120?, 1.6 µm, 2.1 mm X 150 mm, 1/pkg | 186008500 |
CORTECS UPLC T3 Method Validation Kit, 120?, 1.6 µm, 2.1 mm X 100 mm, 3/pkg | 186008536 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 µm, 3 mm X 150 mm, 3/pkg | 186008710 |
CORTECS UPLC T3 Method Validation Kit, 120?, 1.6 µm, 2.1 mm X 150 mm, 3/pkg | 186008537 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 µm, 3 mm X 100 mm, 3/pkg | 186008709 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 µm, 3 mm X 75 mm, 3/pkg | 186008708 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 µm, 3 mm X 50 mm, 3/pkg | 186008707 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 µm, 3 mm X 30 mm, 3/pkg | 186008706 |
CORTECS UPLC T3 Column, 120?, 1.6 µm, 3 mm X 100 mm, 1/pkg | 186008504 |
CORTECS UPLC T3 Column, 120?, 1.6 µm, 3 mm X 150 mm, 1/pkg | 186008505 |
CORTECS UPLC T3 Column, 120?, 1.6 µm, 2.1 mm X 30 mm, 1/pkg | 186008496 |
CORTECS UPLC T3 Column, 120?, 1.6 µm, 2.1 mm X 50 mm, 1/pkg | 186008497 |
CORTECS UPLC T3 Column, 120?, 1.6 µm, 2.1 mm X 100 mm, 3/pkg | 176003894 |
CORTECS UPLC T3 Column, 120?, 1.6 µm, 2.1 mm X 75 mm, 1/pkg | 186008498 |
CORTECS UPLC T3 Column, 120?, 1.6 µm, 2.1 mm X 150 mm, 3/pkg | 176003895 |
CORTECS UPLC T3 Column, 120?, 1.6 µm, 2.1 mm X 100 mm, 1/pkg | 186008499 |
CORTECS UPLC C18 Column, 90?, 1.6 µm, 2.1 mm X 50 mm, 3/pkg | 176003147 |
CORTECS UPLC C18 Column, 90?, 1.6 µm, 2.1 mm X 75 mm, 3/pkg | 176003148 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 µm, 3 mm X 150 mm, 3/pkg | 186007165 |
CORTECS UPLC C18 Column, 90?, 1.6 µm, 3 mm X 100 mm, 1/pkg | 186007100 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 µm, 3 mm X 30 mm, 3/pkg | 186007161 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 µm, 3 mm X 50 mm, 3/pkg | 186007162 |
CORTECS UPLC C18 Column, 90?, 1.6 µm, 3 mm X 150 mm, 1/pkg | 186007102 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 µm, 3 mm X 75 mm, 3/pkg | 186007163 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 µm, 3 mm X 100 mm, 3/pkg | 186007164 |
CORTECS UPLC C18 VanGuard Pre-column, 90?, 1.6 µm, 2.1 mm X 5 mm, 3/pkg | 186007123 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 150 mm, 3/pkg | 186007160 |
CORTECS UPLC C18 Column, 90?, 1.6 µm, 3 mm X 75 mm, 1/pkg | 186007099 |
CORTECS UPLC C18 Column, 90?, 1.6 µm, 3 mm X 50 mm, 1/pkg | 186007098 |
CORTECS UPLC C18 Column, 90?, 1.6 µm, 3 mm X 30 mm, 1/pkg | 186007097 |
CORTECS UPLC C18 Column, 90?, 1.6 µm, 2.1 mm X 75 mm, 1/pkg | 186007094 |
CORTECS UPLC C18 Column, 90?, 1.6 µm, 2.1 mm X 50 mm, 1/pkg | 186007093 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 30 mm, 3/pkg | 186007156 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 50 mm, 3/pkg | 186007157 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 75 mm, 3/pkg | 186007158 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 100 mm, 3/pkg | 186007159 |
CORTECS UPLC C18 Column, 90?, 1.6 µm, 3 mm X 150 mm, 3/pkg | 176003155 |
CORTECS UPLC C18 Column, 90?, 1.6 µm, 3 mm X 100 mm, 3/pkg | 176003154 |
CORTECS UPLC C18 Column, 90?, 1.6 µm, 3 mm X 75 mm, 3/pkg | 176003153 |
CORTECS UPLC C18 Column, 90?, 1.6 µm, 3 mm X 50 mm, 3/pkg | 176003152 |
CORTECS UPLC C18 Column, 90?, 1.6 µm, 3 mm X 30 mm, 3/pkg | 176003151 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 150 mm, 3/pkg | 186007180 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 2.1 mm X 75 mm, 3/pkg | 176003168 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 2.1 mm X 100 mm, 3/pkg | 176003169 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 3 mm X 75 mm, 1/pkg | 186007120 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 2.1 mm X 50 mm, 3/pkg | 176003167 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 2.1 mm X 30 mm, 3/pkg | 176003166 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 3 mm X 150 mm, 1/pkg | 186007122 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 3 mm X 100 mm, 1/pkg | 186007121 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 3 mm X 30 mm, 1/pkg | 186007118 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 3 mm X 50 mm, 1/pkg | 186007119 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 3 mm X 75 mm, 3/pkg | 176003173 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 3 mm X 100 mm, 3/pkg | 176003174 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 3 mm X 30 mm, 3/pkg | 176003171 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 3 mm X 50 mm, 3/pkg | 176003172 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 75 mm, 3/pkg | 186007178 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 100 mm, 3/pkg | 186007179 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 30 mm, 3/pkg | 186007176 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 3 mm X 150 mm, 3/pkg | 176003175 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 50 mm, 3/pkg | 186007177 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 2.1 mm X 30 mm, 1/pkg | 186007113 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 2.1 mm X 150 mm, 1/pkg | 186007117 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 2.1 mm X 150 mm, 3/pkg | 176003170 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 2.1 mm X 100 mm, 1/pkg | 186007116 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 2.1 mm X 75 mm, 1/pkg | 186007115 |
CORTECS UPLC C18+ Column, 90?, 1.6 µm, 2.1 mm X 50 mm, 1/pkg | 186007114 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 µm, 3 mm X 50 mm, 3/pkg | 186007182 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 2.1 mm X 150 mm, 1/pkg | 186007107 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 µm, 3 mm X 30 mm, 3/pkg | 186007181 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 3 mm X 30 mm, 1/pkg | 186007108 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 3 mm X 50 mm, 1/pkg | 186007109 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 µm, 3 mm X 150 mm, 3/pkg | 186007185 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 µm, 3 mm X 100 mm, 3/pkg | 186007184 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 µm, 3 mm X 75 mm, 3/pkg | 186007183 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 3 mm X 150 mm, 3/pkg | 176003165 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 3 mm X 100 mm, 3/pkg | 176003164 |
CORTECS UPLC C18+ VanGuard Pre-column, 90?, 1.6 µm, 2.1 mm X 5 mm, 3/pkg | 186007125 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 3 mm X 30 mm, 3/pkg | 176003161 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 2.1 mm X 50 mm, 1/pkg | 186007104 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 2.1 mm X 150 mm, 3/pkg | 176003160 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 2.1 mm X 30 mm, 1/pkg | 186007103 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 2.1 mm X 100 mm, 1/pkg | 186007106 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 3 mm X 50 mm, 3/pkg | 176003162 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 2.1 mm X 75 mm, 1/pkg | 186007105 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 2.1 mm X 100 mm, 3/pkg | 176003159 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 2.1 mm X 50 mm, 3/pkg | 176003157 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 2.1 mm X 75 mm, 3/pkg | 176003158 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 2.1 mm X 30 mm, 3/pkg | 176003156 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 3 mm X 150 mm, 1/pkg | 186007112 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 3 mm X 100 mm, 1/pkg | 186007111 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 3 mm X 75 mm, 1/pkg | 186007110 |
CORTECS C8 Column, 90?, 1.6 µm, 2.1 mm X 100 mm, 1/pkg | 186008401 |
CORTECS C8 Column, 90?, 1.6 µm, 2.1 mm X 150 mm, 1/pkg | 186008402 |
CORTECS C8 Column, 90?, 1.6 µm, 3 mm X 100 mm, 3/pkg | 176003837 |
CORTECS C8 Column, 90?, 1.6 µm, 2.1 mm X 75 mm, 1/pkg | 186008400 |
CORTECS C8 Column, 90?, 1.6 µm, 2.1 mm X 50 mm, 1/pkg | 186008399 |
CORTECS C8 Column, 90?, 1.6 µm, 2.1 mm X 30 mm, 1/pkg | 186008398 |
CORTECS C8 Column, 90?, 1.6 µm, 2.1 mm X 50 mm, 3/pkg | 176003830 |
CORTECS C8 Column, 90?, 1.6 µm, 2.1 mm X 75 mm, 3/pkg | 176003831 |
CORTECS C8 Column, 90?, 1.6 µm, 2.1 mm X 100 mm, 3/pkg | 176003832 |
CORTECS C8 Column, 90?, 1.6 µm, 2.1 mm X 150 mm, 3/pkg | 176003833 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 100 mm, 3/pkg | 186007169 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 30 mm, 3/pkg | 186007166 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 50 mm, 3/pkg | 186007167 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 75 mm, 3/pkg | 186007168 |
CORTECS UPLC HILIC Column, 90?, 1.6 µm, 3 mm X 75 mm, 3/pkg | 176003163 |
CORTECS UPLC HILIC VanGuard Pre-column, 90?, 1.6 µm, 2.1 mm X 5 mm, 3/pkg | 186007124 |
CORTECS C8 Column, 90?, 1.6 µm, 3 mm X 100 mm, 1/pkg | 186008411 |
CORTECS C8 Column, 90?, 1.6 µm, 2.1 mm X 30 mm, 3/pkg | 176003829 |
CORTECS C8 Column, 90?, 1.6 µm, 3 mm X 150 mm, 1/pkg | 186008412 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 µm, 3 mm X 100 mm, 3/pkg | 186007174 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 µm, 3 mm X 150 mm, 3/pkg | 186007175 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 µm, 3 mm X 50 mm, 3/pkg | 186007172 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 µm, 3 mm X 75 mm, 3/pkg | 186007173 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 150 mm, 3/pkg | 186007170 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 µm, 3 mm X 30 mm, 3/pkg | 186007171 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 2.1 mm X 150 mm, 3/pkg | 176003931 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 100 mm, 3/pkg | 186008699 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 3 mm X 30 mm, 3/pkg | 176003932 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 µm, 2.1 mm X 75 mm, 3/pkg | 186008698 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 µm, 3 mm X 50 mm, 3/pkg | 176003933 |
