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Anti IDH2 (R140Q) Mouse Monoclonal Antibody

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公司名稱武漢紐斯特生物科技有限公司
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cAMP ELISA kit,active抗體，點(diǎn)突變抗體，ELISA試劑盒

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IDH2(R140Q)[26165][100ulCatalogNumber:26165GeneSymbol:IDH2

Anti IDH2 (R140Q) Mouse Monoclonal Antibody 產(chǎn)品信息

IDH2 (R140Q) [26165]
[100ul

Catalog Number: 26165

Gene Symbol: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH

Description: Anti IDH2(R140Q) Mouse Monoclonal Antibody

Background: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.

Immunogen: A synthetic peptide from the internal region of IDH2 which includes the mutation of R140Q, human origin.

Applications: ELISA, WB, IF, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IF: 1:50-1:100
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ ａnd Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes R140Q mutant, but not wild-type IDH2 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant IDH2(R140Q) and wildtype proteins. Purified His-tagged IDH2(R140Q) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti IDH2(R140Q) monoclonal antibody (Cat. #26165).

Immunofluorescence:

Immunofluorescence of cells expressing IDH2 proteins with anti IDH2(R140Q) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R140Q) plasmid (right column), then fixed and stained with anti-IDH2(R140Q) monoclonal antibody (Cat. #26165).

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