博研生物抗體,可應(yīng)用于IHC、WB、 IF、IP、ELISA,質(zhì)量保證,無效免費退換,另外本公司生產(chǎn)經(jīng)營各種標(biāo)記二抗、免疫印跡常用試劑、免疫組化常用試劑,咨詢!
產(chǎn)品名稱:Anti-BMP15抗體,骨形態(tài)發(fā)生蛋白15抗體現(xiàn)貨
產(chǎn)品編號:BYK-6612R
產(chǎn)品規(guī)格: 0.1ml/0.2ml
抗體來源:Rabbit OR Mouse
產(chǎn)品用途:科研實驗,用于免疫組化實驗,WB、IF、elisa實驗
性 狀: Lyophilized or Liquid
濃 度: 1mg/1ml
亞 型: IgG
貯 存: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹:The BMP15 protein is a member of the bone morphogenetic protein family which is part of the transforming growth factor beta superfamily. The transforming growth factor beta superfamily includes large families of growth and differentiation factors. It is thought that BMP15 may be involved in oocyte maturation and follicular development as a homodimer, or by forming heterodimers with a related protein, Gdf9.
相關(guān)標(biāo)記: HRP Biotin Gold RBITC AP FITC Cy3 Cy5 Cy5.5 Cy7 PE PE-Cy3 PE-CY5 PE-CY5.5 PE-CY7 APC Alexa Fluor 350 Alexa Fluor 488 Alexa Fluor 555 Alexa Fluor 647
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Anti-BMP15抗體,骨形態(tài)發(fā)生蛋白15抗體現(xiàn)貨免疫組化:
石蠟包埋組織切片3~4μm 厚度
1.烤片: 將待做切片置于切片架上,于60℃恒溫烤箱中至少烤1 hr;
2.脫蠟: 切片放入盛有二甲苯的容器中脫蠟3次(即二甲苯Ⅰ、Ⅱ、Ⅲ),每次10 min;
3.水化: 切片經(jīng)下行酒精水化,無水乙醇5min,95%乙醇2次(每次2min),85%乙醇2 min;75%乙醇2min,自來水沖洗,ddH2O洗2×2min;
4.抗原修復(fù): 根據(jù)抗體說明書*方法進行抗原修復(fù),常采用高壓、微波(溫度達到98~100℃)或酶消化修復(fù)法,室溫自然冷卻,自來水沖洗,ddH2O洗2×2min,TBS洗滌(2×2min)(具體修復(fù)方法見附1)* 注:有些抗原勿需修復(fù),直接進入第5步封閉。
5.封閉: 滴加試劑B,37℃濕盒孵育30 min;
6.加一抗:滴加用試劑C(選擇合理的工作比例),37℃濕盒孵育2 hr 或4℃過夜;
7.洗滌: TBS-T洗滌(3×5 min);
8.封閉: 滴加試劑B,37℃濕盒孵育10 min;
9.加二抗: 滴加用抗體稀釋液稀釋的*化二抗(試劑D),37℃濕盒中孵育30 min;
10.洗滌: TBS-T洗滌(3×5 min);
11.封閉: 滴加試劑Tween 20,37℃濕盒孵育封閉20 min;
12.加HRP-SA: 滴加用試劑C稀釋的試劑E(1:50~200,終濃度5~20 nM),37℃濕盒中孵育30 min;
13.洗滌: TBS-T洗滌(3×5 min),TBS洗滌(2×5 min);
14.顯色:應(yīng)用DAB溶液(試劑F)顯色;
15.復(fù)染:自來水充分沖洗,復(fù)染,脫水,透明;
16.封片: 待組織標(biāo)本干后,用試劑緩沖甘油封固劑封片;
17.觀察成像: 顯微鏡下觀察成像。
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