革蘭氏染色液
英文名稱:Gram Staining
革蘭氏染色液用途:用于細菌革蘭氏染色試驗
1.涂片經(jīng)火焰固定,加(結(jié)晶紫溶液)溶液A染1分鐘,用清水沖去染液。
2.加(碘液)溶液B染1分鐘,水洗。
3.加(脫色液)溶液C,不時搖動玻片約10-30秒,至無紫色脫落為止,水洗。
4.加溶液D,染1分鐘,水洗。
5.干后油鏡鏡檢。陽性菌呈紫色、陰性菌呈淡紅色。
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