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豬白介素-5(IL-5)ELISA 試劑盒
規(guī) 格:96T/48T
檢測標(biāo)本:血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等
檢測方法:ELISA
檢測類型:酶聯(lián)免疫夾心法
產(chǎn)品的用途:僅供科研課題使用 凡是購買我公司ELISA試劑盒免費代測、提供技術(shù)服務(wù)、5個工作日出實驗結(jié)果。歡迎新老客戶前來: !
豬白介素-5(IL-5)ELISA 試劑盒FOR RESEARCH USE ONLY
Drug Names
Generic Name:豬白介素-5(IL-5)ELISA 試劑盒
This kit can be used for determination of serum, plasma and liquid samples Organization Content.
The experimental principle:
The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.
Materials provided with the kit
Materials provided with the kit | 48determinations | 96 determinations | Storage |
User manual | 1 | 1 |
|
Closure plate membrane | 2 | 2 |
|
Sealed bags | 1 | 1 |
|
Microelisa stripplate | 1 | 1 | 2-8℃ |
Standard:360ng/L | 0.5ml×1 bottle | 0.5ml×1 bottle | 2-8℃ |
Standard diluent | 1.5ml×1 bottle | 1.5ml×1 bottle | 2-8℃ |
HRP-Conjugate reagent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Sample diluent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Chromogen Solution A | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Chromogen Solution B | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Stop Solution | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
wash solution | (20ml×20 fold) ×1bottle | (20ml×30 fold) ×1bottle | 2-8℃ |
Specimen requirements
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separay. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)
2.add sample:Set blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate:Operation with 3.
8.washing:Operation with 5.
9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
豬白介素-5(IL-5)ELISA 試劑盒Important notes
豬白介素-5(IL-5)ELISA 試劑盒Calculate:
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
豬白介素-5(IL-5)ELISA 試劑盒Storage and validity
1.Storage: 2-8℃.
2.validity: six months.
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人抗眼肌抗體(EMAb )ELISA 試劑盒 Human Eye muscle antibody(EMAb ) ELISA Kit
人抗子宮內(nèi)膜抗體(EMAb) ELISA 試劑盒 Human anti-endometrium antibody (EMAb) ELISA Kit
人內(nèi)皮單核細(xì)胞活化多肽II ELISA 試劑盒 Human EMAP-II ELISA Kit
人紅細(xì)胞膜蛋白(EMP)ELISA 試劑盒 Human erythrocyte membrane protein(EMP) ELISA Kit
人可提取核抗原(ENA)ELISA 試劑盒 Human Extractable nucler antigen(ENA) ELISA Kit
人上皮細(xì)胞誘導(dǎo)中型粘細(xì)胞活化因子(ENA) ELISA 試劑盒 Human Epithelial induced neutral colloblast activation factor(ENA) ELISA Kit
人上皮中性粒細(xì)胞活化肽78(ENA-78) ELISA 試劑盒 Human Epithelial neutrophil activating peptide 78(ENA-78) ELISA Kit
人抗可提取核抗原抗體(ENA-Ab) ELISA 試劑盒 Human Extractable nucler antibody(ENA-Ab) ELISA Kit
人內(nèi)皮脂肪酶(EL) ELISA 試劑盒 Human Endothelial lipase(EL) ELISA Kit
人腦啡肽(ENK)ELISA 試劑盒 Human Enkephalin (ENK) ELISA Kit
人內(nèi)皮型一氧化氮合成酶(eNOS) ELISA 試劑盒 Human Endothelial Nitric Oxide Synthase (eNOS) ELISA Kit
人內(nèi)皮型一氧化氮合成酶3 (eNOS-3) ELISA 試劑盒 Human Endothelial Nitric Oxide Synthase 3 (eNOS-3) ELISA Kit
人噬酸性粒細(xì)胞趨化因子 ELISA 試劑盒 Human Eotaxin ELISA Kit
人噬酸性粒細(xì)胞趨化因子2 ELISA 試劑盒 Human Eotaxin-2 ELISA Kit
人噬酸性粒細(xì)胞趨化因子3 ELISA 試劑盒 Human Eotaxin-3 ELISA Kit
人上皮細(xì)胞粘附分子 (Ep-CAM) ELISA 試劑盒 Human Epithelial Cell Adhesion Molecule (Ep-CAM) ELISA Kit
人腎上腺素(EPI)ELISA 試劑盒 Human Epinephrine(EPI) ELISA Kit
人紅細(xì)胞生成素 (EPO)ELISA 試劑盒 Human Erythropoietin (EPO) ELISA Kit
人紅細(xì)胞生成素受體(EPOR) ELISA 試劑盒 Human Erythropoietin Receptor (EPOR) ELISA Kit
人雌激素受體(ER)ELISA 試劑盒 Human Estrogen Receptor(ER) ELISA Kit
人細(xì)胞外信號調(diào)節(jié)激酶(ERK) ELISA 試劑盒 Human Extracellular Signal Regulated Kinase (ERK) ELISA Kit
人內(nèi)皮抑素(ES) ELISA 試劑盒 Human Endostatin (ES) ELISA Kit
人上皮特異性抗原(ESA) ELISA 試劑盒 Human epithelial specific antigen(ESA) ELISA Kit
人E選擇素ELISA 試劑盒 Human E-Selectin ELISA kit
人紅細(xì)胞刺激因子(ESF)ELISA 試劑盒 Human erythropoiesis stimulating factor(ESF) ELISA Kit
人雌二醇(E2)ELISA 試劑盒 Human Estradiol (E2)ELISA Kit
人雌二醇受體(ER) ELISA 試劑盒 Human Estradiol receptor(ER) ELISA Kit
人雌三醇(E3) ELISA 試劑盒 Human Estriol(E3) ELISA Kit
人游離雌三醇(fE3) ELISA 試劑盒 Human free Estriol(fE3) ELISA Kit
人雌激素 誘導(dǎo)蛋白PS2ELISA 試劑盒 Human Estrogen-induced protein pS2 ELISA Kit
人內(nèi)毒素(ET ) ELISA 試劑盒 Human endotoxin(ET ) ELISA Kit
人內(nèi)皮素(ET-1) 1ELISA 試劑盒 Human Endothelin 1 (ET-1) ELISA Kit
人外顯肽ELISA 試劑盒 Human Extein ELISA Kit
人原片段F1+2(F1+2) ELISA 試劑盒 Human Prothrombin Fragment 1+2 (F1+2) ELISA kit
人Ⅲ(F3)ELISA 試劑盒 Human Coagulation Factor III (F3) ELISA Kit
人第八因子相關(guān)抗原 ELISA 試劑盒 Human Factor Ⅷ Related Antigen(FⅧAg) ELISA Kit
人Ⅷ相關(guān)抗原 ELISA 試劑盒 Human coagulation factor Ⅷ related antigen(FⅧ-Ag) ELISA Kit
人ⅩⅢELISA 試劑盒 Human coagulation factor ⅩⅢ(FⅩⅢ) ELISA Kit
人活化ⅫELISA 試劑盒 Human FⅫa ELISA Kit
人葉酸(FA) ELISA 試劑盒 Human Folic acid(FA) ELISA Kit
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