核因子1A抗體 Anti-NFIA/NF-1抗體相關(guān)標(biāo)記     Alexa Fluor 350   Alexa Fluor 488   Alexa Fluor 555   Alexa Fluor 647   AP   APC   Biotin  Cy3   Cy5  
 Cy5.5   Cy7   FITC   Gold   HRP   PE   PE-Cy3   PE-CY5   PE-CY5.5   PE-CY7   RBITC  
英文名稱     Anti-NFIA/NF-1
中文名稱     核因子1A抗體
別    名     CCAAT binding transcription factor; CCAAT Box Binding Transcription Factor; CCAAT-box-binding transcription factor; CTF; FLJ39164; NF I/A; NF-I/A; NF1 A; NF1-A; NF1A; NFI A; NFI L; NFI-A; NFIA; NFIA_HUMAN; Nuclear Factor 1 A; Nuclear factor 1 A type; Nuclear factor 1 A-type; Nuclear factor 1/A; Nuclear Factor 1A; Nuclear Factor I A; Nuclear factor I/A; nuclear factor I/X (CCAAT-binding transcription factor); ynuclear factor I/X (CCAAT-binding transcription factor); Nuclear factor I/X; Nuclear Factor IA; TGGCA binding protein; TGGCA-binding protein. 
抗體來源     Rabbit
克隆類型     polyclonal
 核因子1A抗體 Anti-NFIA/NF-1抗體交叉反應(yīng)     Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep, 
產(chǎn)品類型     一抗  
研究領(lǐng)域    
蛋白分子量     predicted molecular weight: 56kDa
性    狀     Lyophilized or Liquid
免 疫 原     KLH conjugated synthetic peptide derived from human NFIA/NF-1
亞    型     IgG
純化方法     affinity purified by Protein A
儲 存 液     Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產(chǎn)品應(yīng)用      WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500
（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.
保存條件     Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note     This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 抗體產(chǎn)品介紹    NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, and activates transcription through a putatively novel, proline-rich, carboxy terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, alpha-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vilogenin gene promoter.
Function : Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Subunit : Binds DNA as a homodimer. 
Subcellular Location : Nucleus.
Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity : Belongs to the CTF/NF-I family.
Contains 1 CTF/NF-I DNA-binding domain.
Database links : UniProtKB/Swiss-Prot: Q12857.2