細(xì)胞名稱

K-562人慢性髓原白血病細(xì)胞

描述

連續(xù)細(xì)胞株K-562由Lozzio從一位臨終的53歲女性慢性骨髓性白血病患者的胸水中建立。 細(xì)胞被歸入高度未分化的粒性白細(xì)胞類。 Anderson等對(duì)其表面膜性質(zhì)的研究表明，K-562是一株人類紅白血病細(xì)胞。 K-562細(xì)胞株在自然殺傷分析中廣泛作為高敏感的體外受體。 See Pross等將它用于NK細(xì)胞的體外詳細(xì)檢測(cè)，包括NK細(xì)胞活性的數(shù)學(xué)量化。 K-562母細(xì)胞是多能性，造血惡性細(xì)胞，它能自發(fā)分化成可識(shí)別的紅血球祖細(xì)胞、粒性白細(xì)胞、單核細(xì)胞等。它是EBNA陰性的。

動(dòng)物種別

人

性別

女

組織來(lái)源

骨髓；慢性骨髓性白血病(CML)

形態(tài)

淋巴母細(xì)胞

培養(yǎng)基和添加劑

IMDM培養(yǎng)基(GIBCO,貨號(hào)12200036)，90%；優(yōu)質(zhì)胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

REFERENCE

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