細(xì)胞名稱

Calu-1人肺癌細(xì)胞

描述

超微結(jié)構(gòu)特征包括眾多微絨毛，顯著的RER(粗面內(nèi)質(zhì)網(wǎng))，溶酶體，脂包含體，無病毒顆粒。 含ras (H-ras)癌基因。

動物種別

人

性別

男

組織來源

器官： 肺 腫瘤分期： III期 疾?。?表皮瘤 取材轉(zhuǎn)移灶： 肋膜

形態(tài)

上皮細(xì)胞

培養(yǎng)基和添加劑

McCOY's 5A(SIGMA,貨號M4892，添加NaHCO3 2.2g/L)，90%；優(yōu)質(zhì)胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Human tumor cells in vitro. New York: Plenum Press; 1975. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 Tew KD, Taylor DM. Studies with cyclophosphamide labeled with phosphorus-32: nucleic acid alkylation and its effect on DNA synthesis in rat tumor and normal tissues. J. Natl. Cancer Inst. 58: 1413-1418, 1977. PubMed: 857031 Fogh J. C*tion, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047