細(xì)胞名稱

NIH/3T3小鼠胚胎細(xì)胞

描述

與原始的隨機(jī)交配3T3和近交系BALB/c 3T3建株方法一樣，NIH/3T3，是從NIH Swiss小鼠胚胎培養(yǎng)物中建立的高度接觸抑制的連續(xù)細(xì)胞株。 為了培育在形態(tài)學(xué)特征上更適合于進(jìn)行轉(zhuǎn)化分析的亞株，建立的NIH/3T3細(xì)胞株又進(jìn)行了五輪以上亞克隆。 這株細(xì)胞對(duì)DNA轉(zhuǎn)化及轉(zhuǎn)染研究十分有用。 檢測(cè)表明肢骨發(fā)育畸形病毒(鼠痘)陰性。

動(dòng)物種別

小鼠

性別

***

組織來源

胚胎

形態(tài)

成纖維細(xì)胞

培養(yǎng)基和添加劑

DMEM培養(yǎng)基(GIBCO,貨號(hào)12800017，添加NaHCO3 1.5g/L)，90%；優(yōu)質(zhì)胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

REFERENCE

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