細(xì)胞名稱(chēng)

15P-1小鼠睪丸上皮細(xì)胞15P-1（干細(xì)胞庫(kù)保藏）

描述

小鼠睪丸上皮細(xì)胞，每支細(xì)胞量1×10⁶

動(dòng)物種別

小鼠

性別

雄

組織來(lái)源

6月齡的小鼠睪丸

形態(tài)

上皮細(xì)胞

培養(yǎng)基和添加劑

小鼠睪丸上皮細(xì)胞15P-1*培養(yǎng)基配方（100 ml）： 　 DMEM (Invitrogen, 12430) 89 ml 　 FBS (Biochrom, S4115) 10 ml Sodium Pyruvate (Invitrogen, 11360-070) 1 ml（終濃度1 mM） 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。

供應(yīng)限制

A。僅供研究之用。

REFERENCE

Paquis-Flucklinger V, et al. Expression in transgenic mice of the large T antigen of polyomavirus induces Sertoli cell tumours and allows the establishment of differentiated cell lines. Oncogene 8: 2087-2094, 1993. PubMed: 8393161 Rassoulzadegan M, et al. Transmeiotic differentiation of male germ cells in culture. Cell 75: 997-1006, 1993. PubMed: 7504588 Grandjean V, et al. Stage-specific signals in germ line differentiation: control of Sertoli cell phagocytic activity by spermatogenic cells. Dev. Biol. 184: 165-174, 1997. PubMed: 9142992 Grandjean V, et al. Antimicrobial protection of the mouse testis: synthesis of defensins of the cryptdin family. Biol. Reprod. 57: 1115-1122, 1997. PubMed: 9369178 Vincent S, et al. Stage-specific expression of the Kit receptor and its ligand (KL) during male gametogenesis in the mouse: a Kit-KL interaction critical for meiosis. Development 125: 4585-4593, 1998. PubMed: 9778516 Vidal F, et al. Gene trap analysis of germ cell signaling to Sertoli cells: NGF-TrkA mediated induction of Fra1 and Fos by post-meiotic germ cells. J. Cell Sci. 114: 435-443, 2001. PubMed: 11148144