人組織因子途徑抑制物(TFPI)ELISA Kit說(shuō)明書(shū)
Elisa相關(guān)小常識(shí):
酶與底物 酶結(jié)合物是酶與抗體或抗原, 半抗原在交聯(lián)劑作用下聯(lián)結(jié)的產(chǎn)物。是ELISA成敗的關(guān)鍵試劑,它不僅具有抗體抗原特異的免疫反應(yīng),還具有酶促反應(yīng),顯示出生物放大作用,但不同的酶選用不同的底物。
人組織因子途徑抑制物(TFPI)ELISA Kit說(shuō)明書(shū)
Elisa實(shí)驗(yàn)常用方法:
雙抗體夾心法測(cè)定抗原
將抗原免疫*種動(dòng)物獲得的抗體吸附于固相表面;
加抗原,形成抗原-抗體復(fù)合物;
加抗原免疫第二種動(dòng)物獲得的抗體,形成抗體抗原抗體復(fù)合物;
加酶標(biāo)抗抗體(第二種動(dòng)物抗體的抗體);
加底物。底物的降解量=抗原量。
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