Rabbit Anti-RhoA （Rho A protein）
Cat. Number: Rs-1180R
Quantity size: 0.1ml （dilute with pH 7.4 0.01 M PBS or diluent of antibody）
Background: This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Specificity: · anti- RhoA is a rabbit polyclonal antibody unconjugated
· specific for RhoA of human, mouse, rat, pig.
· broadly reactive with mammalian species
· epitope mapping near the amino terminus of RhoA of human, mouse,
rat .
· use for western blotting, elisa, immunoprecipitation
and immunohistochemistry
· affinity chromatography purification, purity :>95%
· Isotype: IgG
·mol wt:21kDa
Application: ·Western blotting: 1:100-500
· Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
