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pRNA-H1.1/Neo

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產品型號SD1203

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所在地區(qū)：上海上海市

聯(lián)系人：李小姐

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產品簡介

pRNA-H1.1/Neo is a Genscript siRNA expression vector. It is designed for mammalian transfection. It carries a Neomycin resistance gene which can be used for establishing stable cell line.

詳細介紹

Description: pRNA-H1.1/Neo is a Genscript siRNA expression vector. It is designed for mammalian transfection. It carries a Neomycin resistance gene which can be used for establishing stable cell line. It uses H1 promoter for siRNA expression.

Download Protocol:tm0169

Forward Sequencing Primer:
DA0013:pRNA-H1 Forward （TAATACGACTCACTATAGGG）

Reverse Sequencing Primer:
DA0012:pRNA Reverse （TAGAAGGCACAGTCGAGG）

Detailed Map:

Polylinker:4700-4723

H1 Promoter:4600-4699

SV40:647-992

Neomycin:1033-1827

pUC ori:2541-3181

Ampicillin:3329-4189

Publications used this GenScript Product:

Orlando Piro and George J. Broze, Jr. Role for the Kunitz-3 Domain of Tissue Factor Pathway Inhibitor-α in Cell Surface Binding. Circulation. Dec 2004; 110 （23） :3567-3572

Asish K. Ghosh, Robert Steele, and Ratna B. Ray. c-myc Promoter-binding Protein 1 （MBP-1） Regulates Prostate Cancer Cell Growth by Inhibiting MAPK Pathway. J. Biol. Chem. Apr 2005; 280 （14） :14325-14330

Roberta Fiume, Irene Faenza, Alessandro Matteucci, Annalisa Astolfi, Marco Vitale, Alberto Maria Marli, and Lucio Cocco. Nuclear PLCbeta 1 affects CD24 expression in murine erythroleukemia cells. J. Biol. Chem. Jun 2005; 280 （25） :24221-24226

Asish K. Ghosh, Robert Steele, and Ratna B. Ray. MBP-1 regulates prostate cancer cell growth by inhibiting MAP kinase pathway. J. Biol. Chem. Apr 2005; 280 （14） :14325-14330

Livigni A, Scorziello A, Agnese S, Adornetto A, Carlucci A, Garbi C, Castaldo I, Annunziato L, Avvedimento EV, Feliciello A. Mitochondrial AKAP121 links cAMP and src signalling to oxidative metabolism. Mol.Biol.Cell. Jan 2006; 17 （1） :263-271

Asish K. Ghsoh, Robert Steele, and Ratna B. Ray. Knockdown of MBP-1 in human prostate cancer cells delays cell cycle progression. J. Biol. Chem. Aug 2006; 281 （33） :23652-23657

Huang Y, Kang BN, Tian J, Liu Y, Luo HR, Hester L, Snyder SH. The cationic amino acid transporters CAT1 and CAT3 mediate NMDA receptor activation-dependent changes in elaboration of neuronal processes via the mammalian target of rapamycin mTOR pathway. J. Neurosci. Jan 2007; 27 （3） :449-458