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High-Affinity GST Resin

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產(chǎn)品型號(hào)L00206

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GenScript High-Affinity GST Resin (L00206) is designed for the rapid, single-step purification of glutathione S-transferases, glutathione-dependent proteins, and recombinant

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Description:

GenScript High-Affinity GST Resin （L00206） is designed for the rapid, single-step purification of glutathione S-transferases, glutathione-dependent proteins, and recombinant derivatives of glutathione S-transferase, including glutathione S-transferase （GST） fusion proteins expressed in E. coli, insect cells, and mammalian cells. High-Affinity GST Resin can purify GST fusion proteins directly from bacterial lysate using. （50% slurry） has a protein binding capacity of over 6 mg GST/ml packed resin.

The GST Fusion Protein Purification Kit （L00207） also includes five disposable columns and five 0.154 g samples of glutathione to facilitate protein purification.

GenScript's Protein Expression and Purification Kit （L00208） provides a set of reagents for customers to use to facilitate the expression and purification of target proteins. pGS-21a vector is designed for cloning, high-level expression, and the convenient purification of proteins. Either of two tags, （His）6 and GST, may be joined to the N- terminus of the fused protein to facilitate its purification. Recombinant enterokinase light chain （EK） is also included in the kit for the cleavage of tags. The recombinant enterokinase （EK） contains a （His）6 tag for easy removal of EK after cleavage using Ni-resin （not included）.

Total Volume:

20 ml of 50% slurry

Key Features:

Easy to perform: GenScript's simple, speedy procedure purifies GST-fusion proteins with minimal hassle.
High capacity: The system can support over 6 mg horse liver GST/ml medium.
Stability: This reusable resin shows no obvious decrease of the binding capacity after three uses.

Examples:

Fig.1. Purification profile using GenScript . Whole proteins （Lane 1） were passed through GST-resin. The flow-through （Lane 2） contains most of the whole proteins. After wash （Lane 3）, GST protein （Lane 4） was eluted.