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IFN-α 2b, Interferon-α 2b, human

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產(chǎn)品簡介

GenScript Interferon (IFN)-α 2b, human produced in E. coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19,269 Da.

詳細(xì)介紹

Full Name

IFN-α 2b, Interferon-α 2b, human

Abbreviated Name-1

rHuIFN-α-2b; rHuIFNα-2b; rHuIFN-a-2b; rHuIFN-alfa-2b; rHuIFNalfa-2b

Abbreviated Name-2

Interferon-α-2b; Interferonα-2b; Interferon-a-2b; Interferon-alfa-2b; Interferonalfa-2b

Description

GenScript Interferon （IFN）-α 2b, human produced in E. coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19,269 Da. The Interferon-alpha?2b gene was obtained from human leukocytes. The rHuIFN-α 2b is purified by proprietary chromatographic techniques.

Source

E. coli

M.W.

19,269 Da

Purity

Greater than 98.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Endotoxin Level

Less than 0.1 ng/µg （IEU/µg） of rHuIFN-α 2b

Specific Activity

The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.6×10⁸/mg.

Storage

Lyophilized rHuIFN-α 2b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIFN-α 2b should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid repeated freeze-thaw cycles.

Formulation

Lyophilized from a （1 mg/ml） solution in containing 2.3 mg Sodium phosphate dibasic and 0.55 mg sodium phosphate monobasic buffer.

Reconstitution

It is recommended to reconstitute the lyophilized rHuIFN-α 2b in sterile 18MΩ-cm H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Quantitation

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% （1 mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.
2. Analysis by RP-HPLC, using a calibrated solution of IFN-alpha 2b as a Reference Standard.

Sequence analysis

The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln. N-terminal methionine has been compley removed enzymatically.