大鼠一氧化氮（NO）ELISA檢測試劑盒●品牌：子科生物ZIKER,美國R&D,美國immonoway,美國sciencell,德國IBL
●產(chǎn)品說明書：可添加在線客服索要原版詳細(xì)操作說明書，或者留下您的，我們會(huì)將產(chǎn)品詳細(xì)說明書發(fā)送至您的！
●價(jià)格：電議,或咨詢在線客服,或者以郵件形式發(fā)到我司zikerbio進(jìn)行詢價(jià)，我們將在*時(shí)間回復(fù)您！深圳子科生物提供大量品牌進(jìn)口原裝，分裝，以及穩(wěn)定性強(qiáng)的國產(chǎn)ELISA試劑盒，性價(jià)比*。咨詢！

深圳子科生物自主研發(fā)酶聯(lián)免疫分析檢測試劑盒，品牌為ZIKER,可以檢測Human，Rat、Mouse、Porcine、Sheep、Monkey、Goat、Bovine、Canine、Rabbit、Chicken、Duck、Fish、Plant  等各種樣本，品種齊全，質(zhì)量保證，免費(fèi)代測、技術(shù)全程支持服務(wù)，我公司長期跟各大高校科研實(shí)驗(yàn)室保持課題合作，經(jīng)驗(yàn)豐富，質(zhì)量穩(wěn)定！我們所有的產(chǎn)品都是我司直接供貨，無中間差價(jià)，直接供貨，讓您以*惠的實(shí)驗(yàn)預(yù)算完成您的實(shí)驗(yàn)！

大鼠一氧化氮（NO）ELISA檢測試劑盒Assay procedure
1.  Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.
2.  Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.
3.  Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.
4.  Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 
5.  Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.
6.  Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.
7.  Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not 
appear uniform, gently tap the plate to ensure thorough mixing.
8.  Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.
Calculation of results
1.This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 
2.First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. 
3.To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 
4.Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.
5.The sensitivity by this assay is 10.0 pg/ml
6.Standard curve
Storage：  2-8℃.
validity： six months.

其他相關(guān)產(chǎn)品如下：

ZIKER-0904R    anti-Melamine  抗三聚氰胺抗體
ZIKER-0237R    Anti-14-3-3  14-3-3 蛋白抗體
ZIKER-0861R    Anti-2,4-D（2,4-Dichlorophenoxyacetic acid）  2，4-二氯苯氧乙酸 /除草劑/激素型除草劑抗體
ZIKER-0861M    Anti-2,4-D（2,4-Dichlorophenoxyacetic acid）  2，4-二氯苯氧乙酸 /除草劑/激素型除草劑單克隆抗體
ZIKER-1126R    Anti-5-HT（5-hydroxytryptamine）  5-羥色胺抗體
ZIKER-1124R    Anti-5-HTR1A (5-HT1A (5-hydroxytryptamine/serotonin receptor 1A; 5HT1a; ADRBRL1; ADRB2RL1; HTR1A)  5-羥色胺受體1A抗體
ZIKER-1125R    Anti-5-HT1B (5-hydroxytryptamine/serotonin receptor 1B)  5-羥色胺受體1B(5-HT/serotonin R1B)抗體
ZIKER-1056R    Anti-5-HTR2A  5-羥色胺受體2A抗體
ZIKER-0526R    Anti-5-LOX (5-lipoxygenase)  5-脂氧合酶抗體
ZIKER-1278R    anti-8-OHdG（8-hydroxy-2-de-axyguanine）  8-羥基脫氧鳥苷
ZIKER-0094R    Anti-AACT-α1 (chymotrypsin-Alpha1)  α-1抗胰糜蛋白酶抗體
ZIKER-0096R    Anti-AAT（Alpha-1 antitiypsin）  α-1抗胰蛋白酶抗體
ZIKER-1229R    Anti-AATF（Apoptosis-antagonizing transcription factor）  拮抗凋亡轉(zhuǎn)錄因子抗體
ZIKER-1224R    Anti-ABCB6（ATP-binding cassette, sub-family B, member 6）  ABCB6抗體
ZIKER-1231R    Anti-ABCG1（ATP-binding cassette）  三磷酸腺苷結(jié)合盒G1抗體
ZIKER-1245R    Anti-ABCG2（ATP-binding cassette 2）  三磷酸腺苷結(jié)合盒G2抗體
ZIKER-0583R    Anti-ABL1（v-abl Abelson murine leukemia viral oncogene homolog 1）  ABL1抗體
ZIKER-1230R    Anti-ABL2（v-abl Abelson murine leukemia viral oncogene homolog 2）  ABL2抗體
ZIKER-0439R    Anti-ACE1( ACE, testis-specific isoform precursor)  血管緊張素轉(zhuǎn)換酶抗體
ZIKER-1004R    
ZIKER-0203R    Anti-ACEI （Angiotensin I Converting Enzyme Inhibitor ）  血管緊張素1轉(zhuǎn)換酶抑制劑抗體
ZIKER-0120R    Anti-Acinus (Apoptotic chromatin condensation Inducer in the nucleus)  腺泡Acinus抗體