產(chǎn)品名稱:Anti-T2R60抗體,味覺受體蛋白家族2亞基60抗體
產(chǎn)品編號(hào):BYK-12042R
英文名稱:Anti-T2R60
中文名稱:味覺受體蛋白家族2亞基60抗體
英文別名:T2R56; TAS2R60; Taste receptor type 2 member 60; T2R60_HUMAN.
英文介紹:T2R60 is a 318 amino acid multi-pass membrane protein that belongs to the G-protein coupled receptor T2R family. T2R60 acts as a receptor that may play a role in the perception of bitterness, and is also thought to be involved in sensing the chemical composition of gastrointestinal content. As a gustducin-linked receptor, the activity of T2R60 may stimulate G alpha (alpha gustducin), mediate PLC beta 2 activation and lead to the gating of TRPM5. While expressed in subsets of taste receptor cells of the tongue and palate epithelium, T2R60 is found exclusively in gustducin-positive cells. The gene that encodes T2R60 contains 957 bases and maps to human chromosome 7q35. Chromosome 7 houses over 1,000 genes, comprises nearly 5% of the human genome and has been linked to Osteogenesis imperfecta, Pendred syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome.
產(chǎn)品規(guī)格:0.1ml/0.2ml
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)(可來(lái)電索取說明)
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-T2R60抗體,味覺受體蛋白家族2亞基60抗體免疫組化操作流程
1 脫蠟和水化:脫蠟前應(yīng)將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘。 a 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b 無(wú)水乙醇中浸泡五分鐘
c 95%乙醇中浸泡五分鐘
d 75%乙醇中浸泡五分鐘
2 抗原修復(fù):用于福爾馬林固定的石蠟包埋組織芯片:
A 抗原熱修復(fù)
a 高壓熱修復(fù) 在沸水中加入EDTA(ph8.0)或0.01m*緩沖溶液(ph6.0).蓋上不銹鋼鍋蓋,但不能鎖定。將玻片置于金屬染色加上,緩慢加壓,是玻片在緩沖液中浸泡五分鐘,然后將蓋子鎖定,小閥門將會(huì)升起來(lái)。10分鐘后除去熱源,置入涼水中,當(dāng)小閥門沉下去后打開蓋子。此方法適用于較難檢測(cè)或核抗原的抗原修復(fù)。
b 沸熱修復(fù) 電爐或水浴鍋加熱0.01*緩沖液(ph6.0)至95℃左右,放入組織芯片加熱10-15分鐘。
C 微波爐加熱 在微波爐里加熱0.01*緩沖液(ph6.0)至沸騰后將組織芯片放入,斷電,間隔5-10分鐘,反復(fù)1-2次。適用的抗原:
Bcl-2.Bax.AR.PR.C-fos.x-jum.z-kit.c-myc.E-cadherin.
ChromograninA.Cyclin.ER.Heatshock.Protein.HPV.Ki-67.MDMZ.P53.P34.P15.P-glycoprotein.PKC.PCNA.ras.Rb.等
B 酶消化方法
常用0.1%*和0.4%*液。*使用前預(yù)熱37℃,消化時(shí)間約為5-30分鐘。適用于被固定遮蔽的抗原:包括 Collagen.GFAP.
Complement.Cytokeratin.C-erB-2.LCA.LN.等
其他優(yōu)勢(shì)產(chǎn)品:
Anti-API5抗體|凋亡抑制因子5抗體
Anti-AQP3抗體|水通道蛋白-3抗體
Anti-Apolipoprotein D抗體|載脂蛋白D抗體
Anti-Alpha-1-Antichymotrypsin抗體|α-1抗胰*/SERPINA3抗體
Anti-alpha 1 Antitrypsin抗體|α-1抗*抗體
Anti-AATF抗體|拮抗凋亡轉(zhuǎn)錄因子抗體
Anti-ABCB6抗體|ATP結(jié)合蛋白家族6抗體
Anti-ABCG1抗體|三磷酸腺苷結(jié)合盒亞家族G1抗體
Anti-ABL2抗體|ABL2蛋白抗體
Anti-ACE抗體|血管緊張素轉(zhuǎn)換酶ACE1抗體
Anti-ACE2抗體|血管緊張素轉(zhuǎn)換酶2抗體
Anti-ACEI抗體|血管緊張素1轉(zhuǎn)換酶抑制劑抗體
Anti-Acinus抗體|Acinus抗體
Anti-Ack1抗體|醋酸激酶1抗體
Anti-ACTH (1-39)抗體|促腎上腺皮質(zhì)激素(1-39)抗體
Anti-ACTH (18-39)|促腎上腺皮質(zhì)激素ACTH(18-39)抗體
Anti-ACTH (7-23)抗體,促腎上腺皮質(zhì)激素ACTH (7-23)抗體
Anti-alpha smooth muscle Actin抗體|肌動(dòng)蛋白α/α Actin抗體
Anti-ADAMTS1抗體|整合素樣金屬蛋白酶與*1型抗體
Anti-ADAMTS7抗體|整合素樣金屬蛋白酶與*1型-7抗體
Anti-beta Adducin抗體|內(nèi)收蛋白抗體
咨詢及訂購(gòu)的方法:
1.可來(lái)電向我們直接咨詢及訂購(gòu)。:021-6034 0820,158 0061 4926
2.可加客服或交談,向我司在線人員咨詢及訂購(gòu)。
3.可通過郵件咨詢及訂購(gòu)。