產品名稱:Anti-mu Crystallin抗體,CRYM蛋白抗體
產品編號:BYK-17900R
英文名稱:Anti-mu Crystallin
中文名稱:CRYM蛋白抗體
英文別名:CRYM;
CRYM_HUMAN;
Crystallin mu;
DFNA 40;
DFNA40;
Ketimine reductase;
Mu crystallin homolog;
Mu-crystallin homolog;
NADP regulated thyroid hormone binding protein;
NADP-regulated thyroid-hormone-binding protein;
OTTHUMP00000115878;
THBP;
Thiomorpholine carboxylate dehydrogenase.
英文介紹:Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2010]
產品規(guī)格:0.1ml/0.2ml
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細胞術等實驗(可來電索取說明)
此產品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-mu Crystallin抗體,CRYM蛋白抗體免疫組化操作流程
1 脫蠟和水化:脫蠟前應將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘。 a 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b 無水乙醇中浸泡五分鐘
c 95%乙醇中浸泡五分鐘
d 75%乙醇中浸泡五分鐘
2 抗原修復:用于福爾馬林固定的石蠟包埋組織芯片:
A 抗原熱修復
a 高壓熱修復 在沸水中加入EDTA(ph8.0)或0.01m*緩沖溶液(ph6.0).蓋上不銹鋼鍋蓋,但不能鎖定。將玻片置于金屬染色加上,緩慢加壓,是玻片在緩沖液中浸泡五分鐘,然后將蓋子鎖定,小閥門將會升起來。10分鐘后除去熱源,置入涼水中,當小閥門沉下去后打開蓋子。此方法適用于較難檢測或核抗原的抗原修復。
b 沸熱修復 電爐或水浴鍋加熱0.01*緩沖液(ph6.0)至95℃左右,放入組織芯片加熱10-15分鐘。
C 微波爐加熱 在微波爐里加熱0.01*緩沖液(ph6.0)至沸騰后將組織芯片放入,斷電,間隔5-10分鐘,反復1-2次。適用的抗原:
Bcl-2.Bax.AR.PR.C-fos.x-jum.z-kit.c-myc.E-cadherin.
ChromograninA.Cyclin.ER.Heatshock.Protein.HPV.Ki-67.MDMZ.P53.P34.P15.P-glycoprotein.PKC.PCNA.ras.Rb.等
B 酶消化方法
常用0.1%*和0.4%*液。*使用前預熱37℃,消化時間約為5-30分鐘。適用于被固定遮蔽的抗原:包括 Collagen.GFAP.
Complement.Cytokeratin.C-erB-2.LCA.LN.等
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