產(chǎn)品名稱：Anti-HLA A2抗體,組織相容性復(fù)合物A2抗體

產(chǎn)品編號：BYK-18070R
英文名稱：Anti-HLA A2
中文名稱：組織相容性復(fù)合物A2抗體
英文別名：Antigen presenting molecule;

HLA A;

HLA A histocompatibility type;

HLA;

HLA class I;

HLA class I histocompatibility antigen A 2 alpha chain;

HLA class I histocompatibility antigen A2 alpha chain;

HLA-A;

HLAA;

Human leukocyte antigen;

Leucocyte antigen class I;

Leukocyte antigen class I A;

Major histocompatibility complex class I A;

Major histocompatibility complex class I;

MHC class I antigen [precursor];

MHC class I antigen A;

MHC class I antigen A*2;

MHC class I antigen A2;

MHC class I antigen HLA A;

MHC class I antigen HLA A heavy chain;

MHC leukocyte antigen.
英文介紹：HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximay 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.

產(chǎn)品規(guī)格：0.1ml/0.2ml

注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細胞術(shù)等實驗（可來電索取說明）
此產(chǎn)品本公司有標記的一抗出售，相關(guān)標記有：Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記

保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-HLA A2抗體,組織相容性復(fù)合物A2抗體免疫組化操作流程
1 脫蠟和水化：脫蠟前應(yīng)將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘。 a 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b 無水乙醇中浸泡五分鐘
c 95%乙醇中浸泡五分鐘
d 75%乙醇中浸泡五分鐘
2 抗原修復(fù)：用于福爾馬林固定的石蠟包埋組織芯片：
A 抗原熱修復(fù)
a 高壓熱修復(fù) 在沸水中加入EDTA（ph8.0）或0.01m*緩沖溶液（ph6.0）.蓋上不銹鋼鍋蓋，但不能鎖定。將玻片置于金屬染色加上，緩慢加壓，是玻片在緩沖液中浸泡五分鐘，然后將蓋子鎖定，小閥門將會升起來。10分鐘后除去熱源，置入涼水中，當(dāng)小閥門沉下去后打開蓋子。此方法適用于較難檢測或核抗原的抗原修復(fù)。
b 沸熱修復(fù) 電爐或水浴鍋加熱0.01*緩沖液（ph6.0）至95℃左右，放入組織芯片加熱10-15分鐘。
C 微波爐加熱 在微波爐里加熱0.01*緩沖液（ph6.0）至沸騰后將組織芯片放入，斷電，間隔5-10分鐘，反復(fù)1-2次。適用的抗原：
Bcl-2.Bax.AR.PR.C-fos.x-jum.z-kit.c-myc.E-cadherin.
ChromograninA.Cyclin.ER.Heatshock.Protein.HPV.Ki-67.MDMZ.P53.P34.P15.P-glycoprotein.PKC.PCNA.ras.Rb.等
B 酶消化方法 
常用0.1%*和0.4%*液。*使用前預(yù)熱37℃，消化時間約為5-30分鐘。適用于被固定遮蔽的抗原：包括 Collagen.GFAP.
Complement.Cytokeratin.C-erB-2.LCA.LN.等

Anti-HLA A2抗體,組織相容性復(fù)合物A2抗體其他優(yōu)勢產(chǎn)品：

byk-1813R 組蛋白去乙?；?抗體 Anti-HDAC2/HD2
byk-11813R *酶抗體 Anti-BTD/Biotinidase/Sp8
byk-11414R 鋅指蛋白318抗體 Anti-ZNF318
byk-1414R 組蛋白去乙?；?抗體 Anti-HDAC1/HD1
byk-1737R 磷酸化組蛋白H1,4樣蛋白抗體 Anti-phospho-Histone H1.4 (Ser27)
byk-0621R 組蛋白H3樣蛋白抗體 Anti-Histone H3-like protein
byk-0620R 組蛋白H3樣抗體 Anti-Histone H3-like protein
byk-0931R 乙酰化組蛋白H1b抗體 Anti-Acetyl-Histone H1.4 (K53)
byk-11413R 睪丸特異激酶底物蛋白抗體 Anti-TSKS
byk-1413R 組蛋白H1抗體 Anti-Histone H1t
byk-0349R 組蛋白H3抗體 Anti-Histone H3
byk-1818R *三聚體核苷結(jié)合蛋白1抗體 Anti-HINT1
byk-11818R 表皮生長因子樣蛋白2抗體 Anti-CELSR2
byk-1447R 缺氧誘導(dǎo)因子2α 抗體HIF-2α  Anti-HIF 2α
byk-11447R 隱花色素蛋白2抗體 Anti-CRY2/Cryptochrome 2
byk-1407R 缺氧誘導(dǎo)因子1β抗體 Anti-HIF-1β/ARNT1
byk-11407R 羥類固醇脫氫酶17β抗體（17β-HSD8） Anti-HSD17B8
byk-0737R 缺氧誘導(dǎo)因子1α 抗體HIF-1α Anti-HIF-1 Alpha
byk-0870R 人類皰疹病毒8抗體 Anti-HHV8/ORF K2
byk-0866R 人類巨細胞病毒抗體 Anti-HHV4/CMV
byk-0338R 庚型肝炎病毒多聚蛋白抗體 Anti-Polyprotein(Hepatitis G Virus)
byk-1025R 肝細胞生長因子抗體 Anti-HGF
byk-11025R 巨軸索神經(jīng)病蛋白GAN抗體 Anti-Gigaxonin

咨詢及訂購的方法：
1.可來電向我們直接咨詢及訂購。：021-6034 0820,158 0061 4926
2.可加客服或交談，向我司在線人員咨詢及訂購。
3.可通過郵件咨詢及訂購。