產(chǎn)品名稱:Anti-IL33抗體,白介素33抗體實(shí)驗(yàn)步驟
產(chǎn)品編號(hào):BYK-10707R
英文名稱:Anti-IL33
中文名稱:白介素33抗體
英文別名:IL-33; Interleukin-33/Nuclear factor from high endothelial venules; IL33; C9orf26; CHROMOSOME 9 OPEN READING FRAME 26; DKFZp586H0523; DVS27; DVS27 related protein; IL 1F11; IL 33; IL1F11; Interleukin 1 family member 11; Interleukin 33; INTERLEUKIN 33 NFHEV; Interleukin 33 precursor; Interleukin33; NF HEV; NFEHEV; NFHEV; Nuclear factor from high endothelial venules; RP11 575C20.2 IL33_HUMAN.
英文介紹:IL33 is a cytokine which belongs to the IL-1 superfamily, and it induces helper T cells to produce type 2 cytokines. This cytokine was previously named NF-HEV 'nuclear factor (NF) in high endothelial venules' (HEVs), as it was originally identified in these specialized cells.<br>
IL33 mediates its biological effects by interacting with the receptors ST2 and IL-1 Receptor Accessory Protein, activating intracellular molecules in the NF-kappaB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-4, IL-5 and IL-13) from polarized Th2 cells. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the the severe pathological changes observed in mucosal organs following administration of IL33.
產(chǎn)品規(guī)格:0.1ml/0.2ml
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)(可來電索取說明)
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-IL33抗體,白介素33抗體實(shí)驗(yàn)步驟免疫組化染色:
SP法
(1) 脫蠟、水化 (2)PBS洗2-3次各5分鐘 (3)3%H2O2(80%甲醇)滴加在
TMA上,室溫靜置10分鐘 (4)PBS洗2-3次各5分鐘 (5)抗原修復(fù)
(6)PBS洗2-3次各5分鐘 (7)滴加正常山羊血清封閉液,室溫20分鐘,甩去多余液體(8)滴加Ι抗50μl,室溫靜置1小時(shí)或4℃過夜或37℃1小時(shí)(9)4℃過夜后需在37℃復(fù)溫45分鐘(10)PBS洗3次每次2分鐘
(11)滴加Ⅱ抗45-50μl,室溫靜置或37℃1小時(shí)(12)Ⅱ抗中可加入0.05%的
tween-20.(13)PBS洗3次各5分鐘(14)DAB顯色5-10分鐘,在顯微鏡下掌握染色程度 (15)PBS或自來水沖洗10分鐘 (16)蘇木精復(fù)染2分鐘,鹽酸酒精分化(17)自來水沖洗10-15分鐘 (18)脫水、透明、封片、鏡檢。
SABC法
(1) 脫蠟、水化 (2)PBS洗2次各5分鐘 (3)用蒸餾水或PBS配制新鮮的3%H2O2,室溫封閉5-10分鐘,用蒸餾水洗3次(4)抗原修復(fù)
(5) PBS洗5分鐘 (6)滴加正常山羊血清封閉液,室溫20分鐘,甩去多余液體(7)滴加Ι抗50μl,室溫靜置1小時(shí)或4℃過夜或37℃1小時(shí)
(8)PBS洗3次各2分鐘(9)滴加*化Ⅱ抗,20℃-37℃ 20分鐘
(10)PBS洗3次各2分鐘 (11)滴加試劑SABC 20℃-30℃ 20分鐘
(12) PBS洗4次各5分鐘 (13) DAB顯色:試劑盒或自配顯色劑顯色
(14)脫水、透明、封片、鏡檢。
Anti-IL33抗體,白介素33抗體實(shí)驗(yàn)步驟其他優(yōu)勢(shì)產(chǎn)品:
byk-0172R 胰島素受體底物-1抗體 Anti-IRS1
byk-10172R c-fos抗體 Anti-c-fos
byk-0242R 胰島素受體底物p53蛋白抗體 Anti-IRSp53/BAIAP2
byk-2634R 細(xì)胞表面免疫球蛋白樣轉(zhuǎn)錄因子2抗體 Anti-ILT2/CD85j
byk-2500R 干擾素調(diào)節(jié)因子4抗體 Anti-IRF4
byk-12500R 載脂蛋白L5抗體 Anti-APOL5/Apolipoprotein L5
byk-2595R 白介素1受體2抗體 Anti-IL-1R2 /CD121b/IL1 Receptor II
byk-2594R 白介素1受體1抗體 Anti-IL-1R1/CD121a/IL1 Receptor I
byk-2440R 白介素-1受體相關(guān)激酶4抗體 Anti-IRAK4
byk-12440R 膽汁酸鹽輸出泵/ATP結(jié)合盒轉(zhuǎn)運(yùn)蛋白11抗體 Anti-BSEP/ABCB11
byk-1427R 白介素-1受體相關(guān)激酶2抗體 Anti-IRAK2
byk-11427R 轉(zhuǎn)錄激活蛋白CRSP9抗體 Anti-CRSP9
byk-1404R 干擾素調(diào)節(jié)因子1抗體 Anti-IRF-1/MAR1
byk-11404R G蛋白偶聯(lián)受體106抗體 Anti-GPR106
byk-11109R 原鈣粘附蛋白12/血管內(nèi)皮鈣粘蛋白2抗體 Anti-PCDH12
byk-1109R 支架蛋白抗體 Anti-IQGAP1
byk-1274R 整合素αE抗體Integrin αE Anti-Integrin alpha E/CD103
byk-11274R 過敏毒素C4/補(bǔ)體C4抗體 Anti-Complement C4/C4
byk-11356R 神經(jīng)元突觸膜胞外分泌調(diào)節(jié)蛋白1抗體 Anti-RIM1/ABCA4
byk-1356R 整合素αVβ5抗體 Anti-Integrin Alpha V + Beta 5
byk-11310R 動(dòng)力蛋白輕鏈 Anti-TC1/DYNLT1
byk-1310R 整合素αVβ3抗體 Anti-Integrin Alpha V + Beta 3 (CD51+CD61)
byk-2016R 整合素αVβ1抗體 Anti-Integrin Alpha V + Beta1
咨詢及訂購的方法:
1.可來電向我們直接咨詢及訂購。:021-6034 0820,158 0061 4926
2.可加客服或交談,向我司在線人員咨詢及訂購。
3.可通過郵件咨詢及訂購。