產(chǎn)品名稱(chēng):Anti-Zhangfei抗體,宿主細(xì)胞因子Zhangfei抗體*品牌
產(chǎn)品編號(hào):BYK-19162R
英文名稱(chēng):Anti-Zhangfei
中文名稱(chēng):宿主細(xì)胞因子Zhangfei抗體
英文別名:1110034C16Rik;
6330417B10Rik;
AI225749;
AI606244;
CREB/ATF bZIP transcription factor;
Crebzf;
FLJ94018;
HCF-binding transcription factor Zhangfei;
Host cell factor-binding transcription factor Zhangfei;
LAZip;
LAZipII;
SHP-interacting leucine zipper protein;
SMILE;
ZF;
ZHANG_HUMAN.
英文介紹:Host cell factor (HCF) is a cellular cofactor required for the activation of VP16 which expresses the herpes simplex virus immediate early gene. V16 binds to HCF through a 4-amino acid motif similar to the HCF binding domain of the basic leucine-zipper proteins Luman and Zhangfei (ZF). Luman activates promoters containing cAMP or unfolded protein response elements (UPRE). Zhangfei suppresses the transcriptional activity of Luman, but requires HCF binding which may target Luman and Zhangfei to a common location. Sequence analysis predicts that the deduced 272-amino acid Zhangfei protein has a negatively charged N terminus, a leucine zipper of 6 heptad leucine repeats separated by a conserved 6-amino acid spacer, a basic domain, and a bZIP region. It is also presumed that the N-terminal acidic region of Zhangfei is an activation domain.
產(chǎn)品規(guī)格:0.1ml/0.2ml
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)(可來(lái)電索取說(shuō)明)
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-Zhangfei抗體,宿主細(xì)胞因子Zhangfei抗體*品牌免疫組化染色:
SP法
(1) 脫蠟、水化 (2)PBS洗2-3次各5分鐘 (3)3%H2O2(80%甲醇)滴加在
TMA上,室溫靜置10分鐘 (4)PBS洗2-3次各5分鐘 (5)抗原修復(fù)
(6)PBS洗2-3次各5分鐘 (7)滴加正常山羊血清封閉液,室溫20分鐘,甩去多余液體(8)滴加Ι抗50μl,室溫靜置1小時(shí)或4℃過(guò)夜或37℃1小時(shí)(9)4℃過(guò)夜后需在37℃復(fù)溫45分鐘(10)PBS洗3次每次2分鐘
(11)滴加Ⅱ抗45-50μl,室溫靜置或37℃1小時(shí)(12)Ⅱ抗中可加入0.05%的
tween-20.(13)PBS洗3次各5分鐘(14)DAB顯色5-10分鐘,在顯微鏡下掌握染色程度 (15)PBS或自來(lái)水沖洗10分鐘 (16)蘇木精復(fù)染2分鐘,鹽酸酒精分化(17)自來(lái)水沖洗10-15分鐘 (18)脫水、透明、封片、鏡檢。
SABC法
(1) 脫蠟、水化 (2)PBS洗2次各5分鐘 (3)用蒸餾水或PBS配制新鮮的3%H2O2,室溫封閉5-10分鐘,用蒸餾水洗3次(4)抗原修復(fù)
(5) PBS洗5分鐘 (6)滴加正常山羊血清封閉液,室溫20分鐘,甩去多余液體(7)滴加Ι抗50μl,室溫靜置1小時(shí)或4℃過(guò)夜或37℃1小時(shí)
(8)PBS洗3次各2分鐘(9)滴加*化Ⅱ抗,20℃-37℃ 20分鐘
(10)PBS洗3次各2分鐘 (11)滴加試劑SABC 20℃-30℃ 20分鐘
(12) PBS洗4次各5分鐘 (13) DAB顯色:試劑盒或自配顯色劑顯色
(14)脫水、透明、封片、鏡檢。
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