產(chǎn)品名稱:Anti-Nanos3抗體,骨巢蛋白抗體
產(chǎn)品編號:BYK-10227R
英文名稱:Anti-Nanos3
中文名稱:骨巢蛋白抗體
英文別名:NANOS3; NANOS 3; NANOS-3; NOS3; NOS-3; Nanos homolog 3; NANO3_HUMAN.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細(xì)胞術(shù)等實驗(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-Nanos3抗體,骨巢蛋白抗體英文介紹:Nidogens are highly conserved proteins present in vertebrate and invertebrate basement membranes. Nidogens connect the Laminin and Collagen IV networks and integrate other proteins into the membrane. In mammals, two Nidogen proteins, Nidogen and Nidogen-2, interact at comparable levels with Collagen I, IV and Perlecan, serving to stabilize basement membranes and playing a major role in embryogenesis. The two isoforms have a similar shape, consisting of three globular domains, and co-localize in vessel walls and other basement membrane zones. Nidogen-2 is a cell adhesion protein glycosylated at nitrogen and oxygen sites, and is widely distributed in basement membranes in heart, placenta, bone and, to a lesser extent, in pancreas, kidney and skeletal muscle.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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Anti-Nanos3抗體,骨巢蛋白抗體抗體溶解方法及分裝:
1、購買抗體后,查看標(biāo)簽上的規(guī)格,如:0.1ml、0.2ml
2、以規(guī)格為 0.1ml 的凍干粉抗體為例(部分抗體為液體,無需再溶解):
溶解方法一:用移液器加 0.1ml 雙蒸水到試管內(nèi),混勻,-20℃保存,若為其它規(guī)格(如:0.2ml)的凍干粉抗體,則加與標(biāo)簽上規(guī)格*的(如:0.2ml)雙蒸水溶解,
混勻,-20℃保存。
溶解方法二:用移液器加 0.05ml(規(guī)格的 50%)雙蒸水到試管內(nèi),再加 0.05ml(規(guī)格的 50%)
的甘油到試管內(nèi),混勻,-20℃保存,若為其它規(guī)格(如:0.2ml)的凍干粉抗體,則加標(biāo)簽上規(guī)格的 50%(如:0.1ml)雙蒸水,再加
標(biāo)簽上規(guī)格的 50%(如:0.1ml)甘油溶解,混勻,-20℃保存。
抗體溶解后,將其分裝成 5-10 支(以 0.1ml 抗體為例,20ulx5 支或 10ulx10 支), -20℃保存,用
一支取一支,避免反復(fù)凍溶。
另:預(yù)試驗可做幾個梯度 1:100、1:200、1:400 稀釋,如果背景高,還可再稀釋,選一個zui
佳的稀釋比例,再正式做,效果。
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