The diagnosis of intestinal parasitic infections requires appropriate collection, transportation, concentration, staining and identification of parasites from fecal specimens5,6.
This kit filters the concentrate of protozoan cysts, oocysts, and helminth eggs and larvae from fecal material.
- Ready to use
- Compatible with most fecal transportation vials
This kit utilizes existing sedimentation methodology along with standardized components to achieve recovery of protozoan cysts, oocysts and helminth eggs and larvae from preserved fecal specimens. Pore diameters in the filters are 600 microns, allowing passage of cysts, eggs and larvae while retaining macroscopic fecal matter and debris on top1. The surfactant, 10% Triton X-100, reduces surface tension to enhance filtration and frees additional helminth eggs that may be trapped in fecal debris or mucus. Ethyl acetate or xylene substitutes dissolve any fat present in the specimen and aid in the separation of fecal debris from concentrated sediment containing parasites. Ideally 0.5 to 1.0 mL of fecal sediments should remain for adequate recovery and examination of parasites.
The most current sedimentation techniques involve filtering, centrifugation, and defatting steps. The earlier used Ritchie formalin-ether sedimentation technique has been modified, in which ether is replaced with other defatting agents such as ethyl acetate or xylene substitutes to avoid safety issues associated with ether2-4.
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
