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聯(lián)系我時(shí),請(qǐng)告知來(lái)自 環(huán)保在線“Anti-TNF-alpha(1F6)抗體,腫瘤壞死因子-α單克隆抗體"2A抗體規(guī)格0.1ml、0.2ml、1ml,抗體產(chǎn)品有效期一年,質(zhì)保三個(gè)月,質(zhì)保期內(nèi)出現(xiàn)任何質(zhì)量問(wèn)題或是出不了實(shí)驗(yàn)結(jié)果均可免費(fèi)退換:
上海研盟生物科技有限公司Anti-TNF-alpha(1F6)抗體,腫瘤壞死因子-α單克隆抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說(shuō)明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?/p>
簡(jiǎn)單介紹:
中文名稱:腫瘤壞死因子-α單克隆抗體
英文名稱:Anti-TNF-alpha(1F6)
產(chǎn)品編號(hào):byk-0387M
產(chǎn)品別名:tumor necrosis factor-α; APC1; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF a ; TNF alpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precurso; Tumour Necrosis Factor Alpha.
產(chǎn)品規(guī)格:0.1ml、0.2ml、1ml
產(chǎn)品用途:科研實(shí)驗(yàn)
產(chǎn)品價(jià)格:請(qǐng)報(bào)價(jià)
說(shuō)明書:請(qǐng)?zhí)砑涌头苯铀魅?/p>
抗體來(lái)源:該指標(biāo)有兩種產(chǎn)品,一種是兔來(lái)源抗體,一種是鼠來(lái)源抗體
克隆類型:兔來(lái)源為多克隆抗體,鼠來(lái)源單克隆抗體
交叉反應(yīng):請(qǐng)索取說(shuō)明書查看
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲(chǔ) 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-TNF-alpha(1F6)抗體,腫瘤壞死因子-α單克隆抗體產(chǎn)品介紹:Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
免疫印跡法是一種通過(guò)凝膠電泳分離蛋白質(zhì)并通過(guò)電轉(zhuǎn)移把蛋白質(zhì)轉(zhuǎn)移到吸附膜上。一經(jīng)蛋白印跡后,即可通過(guò)特異性的標(biāo)記抗體檢測(cè)到相應(yīng)蛋白質(zhì)。
十二烷基硫酸鈉-聚丙烯酰胺凝膠電泳(SDS-PAGE)可用于檢測(cè)已被SDS化學(xué)變性的蛋白。然而,某些特定的抗體不會(huì)在一個(gè)變性的蛋白質(zhì)分子上識(shí)別其抗原表位,這時(shí)需要進(jìn)行不含SDS的聚丙烯酰胺凝膠電泳。
蛋白印跡轉(zhuǎn)膜可以通過(guò)濕法或半干法進(jìn)行。在前者,凝膠夾在印跡膜和各種過(guò)濾裝置中間,然后把整個(gè)裝置埋進(jìn)一個(gè)裝滿Tris轉(zhuǎn)移緩沖的電極槽中。在后者,夾在中間的凝膠周圍只有少量的緩沖液,然后封閉在電極板之間。雖然半干法轉(zhuǎn)膜較快,但需要很好地?cái)M合各部分的夾層配件,而濕法轉(zhuǎn)膜很少會(huì)出現(xiàn)問(wèn)題,可以盡量保持重復(fù)實(shí)驗(yàn)結(jié)果的*性。此外,如果半干法轉(zhuǎn)膜時(shí)間過(guò)長(zhǎng),較小分子量的蛋白質(zhì)可能會(huì)從膜上轉(zhuǎn)過(guò)。
轉(zhuǎn)膜后,需對(duì)膜進(jìn)行封閉,以減少非特異性蛋白結(jié)合,然后進(jìn)行用一抗孵育膜,以研究感興趣的目標(biāo)蛋白。單克隆抗體通常具有更高的特異性;然而許多單克隆抗體產(chǎn)生于某個(gè)特定肽段而不是一個(gè)天然蛋白質(zhì),因此可能無(wú)法檢測(cè)到PAGE所分離的天然蛋白。也可使用多克隆抗體,但易形成較高的背景值。因一抗通常無(wú)標(biāo)記,所以需要一個(gè)可以結(jié)合在一抗Fc段的標(biāo)記的二抗,以用于zui后的檢測(cè)。通常會(huì)用酶來(lái)標(biāo)記二抗。酶標(biāo)記后的印跡可通過(guò)化學(xué)發(fā)光酶底物及放射自顯影技術(shù)成像。被抗體檢測(cè)和標(biāo)記到的蛋白質(zhì)會(huì)出現(xiàn)在圖像的暗區(qū)。
Anti-TNF-alpha(1F6)抗體,腫瘤壞死因子-α單克隆抗體相關(guān)產(chǎn)品:
CXCL14/BRAK(C-X-C motif chemokine 14) CXC趨化因子14抗體
CXCL16(CXC-chemokine ligand 16) CXC趨化因子16抗體
Secretin 分泌素抗體
Secretin receptor 分泌素受體抗體
SEL1L(Suppressor of lin-12-like Protein) SEL1L抗體
Selenoprotein W 硒蛋白W抗體
Sema3A (semaphorin 3A) 臂板蛋白3A抗體
Sema3F (semaphorin 3F) 臂板蛋白3F抗體
Sema4C (semaphorin 4C) 臂板蛋白4C抗體
SFRP1(Secreted frizzled related protein 1) 分泌型卷曲相關(guān)蛋白1抗體
SGC (soluble glycoprotein C) 可溶性糖蛋白C抗體
SGLT1 (sodium-glucose cotransporter1) 鈉-葡萄糖共轉(zhuǎn)運(yùn)載體1抗體
Shank1(SH3 and multiple anleyrin repead domins protein 1) shank-1抗體
Shadow/SPRN(Shadow of prion protein precuBYKor) 新朊蛋白/朊蛋白相關(guān)蛋白/沙杜(Shadoo)蛋白抗體
SHBG (Sex Hormone Binding Globulin) 性激素結(jié)合球蛋白抗體
Shiga-like toxin IIe variant subunit A[Escherichia coli 0139] 抗大腸桿菌志賀樣毒素Ⅱ型突變體(O139菌型)抗體
Shh(Sonic hedgehog) Shh信號(hào)轉(zhuǎn)導(dǎo)通路膜蛋白受體抗體
SHP-1 (Src homology phosphatase-1) 造血細(xì)胞磷酸酶抗體
SHP2/PTPN11(Src homology 2 (SH2) domain containing phosphotyrosinephosphatase 2) 蛋白*磷酸酶2抗體
SIP1 (survival of motor neuron protein interacting protein 1) 運(yùn)動(dòng)神經(jīng)元存活蛋白結(jié)合蛋白1抗體
SIRT1(sirtuin 1) 沉默調(diào)節(jié)蛋白1抗體
Skp2 (S-phase kinase-associated protein 2) 細(xì)胞S相激酶相關(guān)蛋白抗體
SLC4A4(solute carrier family 4:sodium bicarbonate cotransporter NBC1) *協(xié)同轉(zhuǎn)運(yùn)蛋白4-A4抗體
Slc4a7/Nbcn1(solute carrier family 4, sodium bicarbonate cotransporter, member 7) *協(xié)同轉(zhuǎn)運(yùn)蛋白4-A7抗體
Slc22A17 (solute carrier famili 22A17) 可溶性載質(zhì)轉(zhuǎn)運(yùn)蛋白22A17抗體
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