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聯(lián)系我時,請告知來自 環(huán)保在線“Anti-GDNF Receptor alpha 2抗體,膠質(zhì)細(xì)胞系源性神經(jīng)營養(yǎng)因子受體α2抗體"2A抗體規(guī)格0.1ml、0.2ml、1ml,抗體產(chǎn)品有效期一年,質(zhì)保三個月,質(zhì)保期內(nèi)出現(xiàn)任何質(zhì)量問題或是出不了實(shí)驗(yàn)結(jié)果均可免費(fèi)退換:
上海研盟生物科技有限公司Anti-GDNF Receptor alpha 2抗體,膠質(zhì)細(xì)胞系源性神經(jīng)營養(yǎng)因子受體α2抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?/p>
簡單介紹:
中文名稱:膠質(zhì)細(xì)胞系源性神經(jīng)營養(yǎng)因子受體α2抗體
英文名稱:Anti-GDNF Receptor alpha 2
產(chǎn)品編號:byk-0054R
產(chǎn)品別名:GDNF family receptor alpha-2; GDNF Receptor α2; GDNF receptor beta; GDNFR beta; GDNFR-beta; GDNFRB; GFR alpha 2; GFRA 2; GFRA2; GFR alpha-2; Glial cell line derived neurotrophic factor family receptor alpha2b; Glial cell line derived neurotrophic factor receptor beta; Neurturin receptor alpha; NRTNR alpha; NRTNR-alpha; NTNR alpha; NTNR-alpha; NTNRA; PI linked cell surface accessory protein; RET ligand 2; RETL 2; RETL2; TGF beta related neurotrophic factor receptor 2; TRN receptor GPI anchored; TRNR 2; TRNR2; GFRA2_HUMAN; GDNF family receptor alpha-2; GDNF receptor alpha-2; Short=GDNFR-alpha-2; GFR-alpha-2; Neurturin receptor alpha; TGF-beta-related neurotrophic factor receptor 2.
產(chǎn)品規(guī)格:0.1ml、0.2ml、1ml
產(chǎn)品用途:科研實(shí)驗(yàn)
產(chǎn)品價(jià)格:請報(bào)價(jià)
說明書:請?zhí)砑涌头苯铀魅?/p>
抗體來源:該指標(biāo)有兩種產(chǎn)品,一種是兔來源抗體,一種是鼠來源抗體
克隆類型:兔來源為多克隆抗體,鼠來源單克隆抗體
交叉反應(yīng):請索取說明書查看
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-GDNF Receptor alpha 2抗體,膠質(zhì)細(xì)胞系源性神經(jīng)營養(yǎng)因子受體α2抗體產(chǎn)品介紹:Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. Multiple transcript variants encoding different isoforms have been found for this gene. Members of the glial cell line-derived neurotrophic factor(GDNF) family, including GDNF and neurturin (NTN),play key roles in the control of vertebrate neuron survivaland differentiation. Physiological responses to NTN requirethe presence of a novel glycosylphosphadidylinositollinked protein NTNRa, which is a cell surface receptor forNTN. The cDNAs encoding NTNRa from human, rat,chicken, and mouse have been cloned recently. NTNRa was also termed GDNFRb, Ret ligand 2 (RETL2)or TGF-b-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRa-2 recently. GFRa-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus, NTN, GFRa-2, and the Ret PTK form a complex to transduce NTN signal andto mediate NTN function.
免疫印跡法是一種通過凝膠電泳分離蛋白質(zhì)并通過電轉(zhuǎn)移把蛋白質(zhì)轉(zhuǎn)移到吸附膜上。一經(jīng)蛋白印跡后,即可通過特異性的標(biāo)記抗體檢測到相應(yīng)蛋白質(zhì)。
十二烷基硫酸鈉-聚丙烯酰胺凝膠電泳(SDS-PAGE)可用于檢測已被SDS化學(xué)變性的蛋白。然而,某些特定的抗體不會在一個變性的蛋白質(zhì)分子上識別其抗原表位,這時需要進(jìn)行不含SDS的聚丙烯酰胺凝膠電泳。
蛋白印跡轉(zhuǎn)膜可以通過濕法或半干法進(jìn)行。在前者,凝膠夾在印跡膜和各種過濾裝置中間,然后把整個裝置埋進(jìn)一個裝滿Tris轉(zhuǎn)移緩沖的電極槽中。在后者,夾在中間的凝膠周圍只有少量的緩沖液,然后封閉在電極板之間。雖然半干法轉(zhuǎn)膜較快,但需要很好地?cái)M合各部分的夾層配件,而濕法轉(zhuǎn)膜很少會出現(xiàn)問題,可以盡量保持重復(fù)實(shí)驗(yàn)結(jié)果的*性。此外,如果半干法轉(zhuǎn)膜時間過長,較小分子量的蛋白質(zhì)可能會從膜上轉(zhuǎn)過。
轉(zhuǎn)膜后,需對膜進(jìn)行封閉,以減少非特異性蛋白結(jié)合,然后進(jìn)行用一抗孵育膜,以研究感興趣的目標(biāo)蛋白。單克隆抗體通常具有更高的特異性;然而許多單克隆抗體產(chǎn)生于某個特定肽段而不是一個天然蛋白質(zhì),因此可能無法檢測到PAGE所分離的天然蛋白。也可使用多克隆抗體,但易形成較高的背景值。因一抗通常無標(biāo)記,所以需要一個可以結(jié)合在一抗Fc段的標(biāo)記的二抗,以用于zui后的檢測。通常會用酶來標(biāo)記二抗。酶標(biāo)記后的印跡可通過化學(xué)發(fā)光酶底物及放射自顯影技術(shù)成像。被抗體檢測和標(biāo)記到的蛋白質(zhì)會出現(xiàn)在圖像的暗區(qū)。
Anti-GDNF Receptor alpha 2抗體,膠質(zhì)細(xì)胞系源性神經(jīng)營養(yǎng)因子受體α2抗體相關(guān)產(chǎn)品:
CXCL14/BRAK(C-X-C motif chemokine 14) CXC趨化因子14抗體
CXCL16(CXC-chemokine ligand 16) CXC趨化因子16抗體
Secretin 分泌素抗體
Secretin receptor 分泌素受體抗體
SEL1L(Suppressor of lin-12-like Protein) SEL1L抗體
Selenoprotein W 硒蛋白W抗體
Sema3A (semaphorin 3A) 臂板蛋白3A抗體
Sema3F (semaphorin 3F) 臂板蛋白3F抗體
Sema4C (semaphorin 4C) 臂板蛋白4C抗體
SFRP1(Secreted frizzled related protein 1) 分泌型卷曲相關(guān)蛋白1抗體
SGC (soluble glycoprotein C) 可溶性糖蛋白C抗體
SGLT1 (sodium-glucose cotransporter1) 鈉-葡萄糖共轉(zhuǎn)運(yùn)載體1抗體
Shank1(SH3 and multiple anleyrin repead domins protein 1) shank-1抗體
Shadow/SPRN(Shadow of prion protein precuBYKor) 新朊蛋白/朊蛋白相關(guān)蛋白/沙杜(Shadoo)蛋白抗體
SHBG (Sex Hormone Binding Globulin) 性激素結(jié)合球蛋白抗體
Shiga-like toxin IIe variant subunit A[Escherichia coli 0139] 抗大腸桿菌志賀樣毒素Ⅱ型突變體(O139菌型)抗體
Shh(Sonic hedgehog) Shh信號轉(zhuǎn)導(dǎo)通路膜蛋白受體抗體
SHP-1 (Src homology phosphatase-1) 造血細(xì)胞磷酸酶抗體
SHP2/PTPN11(Src homology 2 (SH2) domain containing phosphotyrosinephosphatase 2) 蛋白*磷酸酶2抗體
SIP1 (survival of motor neuron protein interacting protein 1) 運(yùn)動神經(jīng)元存活蛋白結(jié)合蛋白1抗體
SIRT1(sirtuin 1) 沉默調(diào)節(jié)蛋白1抗體
Skp2 (S-phase kinase-associated protein 2) 細(xì)胞S相激酶相關(guān)蛋白抗體
SLC4A4(solute carrier family 4:sodium bicarbonate cotransporter NBC1) *協(xié)同轉(zhuǎn)運(yùn)蛋白4-A4抗體
Slc4a7/Nbcn1(solute carrier family 4, sodium bicarbonate cotransporter, member 7) *協(xié)同轉(zhuǎn)運(yùn)蛋白4-A7抗體
Slc22A17 (solute carrier famili 22A17) 可溶性載質(zhì)轉(zhuǎn)運(yùn)蛋白22A17抗體
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