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更新時(shí)間:2017-10-27 13:46:11瀏覽次數(shù):243次
聯(lián)系我時(shí),請(qǐng)告知來自 環(huán)保在線上海研盟生物科技有限公司“Anti-Laminin Beta 1抗體,層粘連蛋白β1抗體"抗體規(guī)格0.1ml、0.2ml、1ml,抗體產(chǎn)品有效期一年,質(zhì)保三個(gè)月,質(zhì)保期內(nèi)出現(xiàn)任何質(zhì)量問題或是出不了實(shí)驗(yàn)結(jié)果均可免費(fèi)退換。:、
上海研盟生物科技有限公司Anti-Laminin Beta 1抗體,層粘連蛋白β1抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取。:
中文名稱:層粘連蛋白β1抗體
英文名稱:Anti-Laminin Beta 1抗體
產(chǎn)品編號(hào):BYK-1526R
別名:LAMA 1 LAMA; LAMA1; LAMB1; CLM; Cutis laxa with marfanoid phenotype; LAM B1; LAMB 1; LAMB1_HUMAN; Laminin B1; Laminin beta 1 chain; Laminin beta 1 chain precursor; Laminin beta1; Laminin subunit beta-1; Laminin-1 subunit beta; Laminin-10 subunit beta; Laminin-12 subunit beta; Laminin-2 subunit beta; Laminin-6 subunit beta; Laminin-8 subunit beta; MGC142015; Laminin β1;Laminin A chain; Laminin alpha 1; Laminin alpha 1 chain; Laminin B1 chain; Laminin subunit beta 1.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來源:本公司抗體有兔來源和鼠來源等
克隆類型:兔來源為多抗,鼠來源為單抗
產(chǎn)品類型:一抗
Anti-Laminin Beta 1抗體,層粘連蛋白β1抗體產(chǎn)品介紹:Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm(EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis.
上海研盟生物科技有限公司W(wǎng)estern Blotting 問題解答
1、膠片上存在反轉(zhuǎn)印象(如:亮的條帶、黑的背景)?
原因:HRP的濃度過高;
解決:稀釋HRP至少10倍。
2、膜上有黃色或棕色的沉積?
原因:HRP的濃度過高;
解決:稀釋HRP至少10倍。
3、在暗室中條帶過于明亮?
原因:HRP的濃度過高;
解決:稀釋HRP至少10倍。
4、條帶的發(fā)光時(shí)間持續(xù)了至少8小時(shí)?
原因:HRP的濃度過高;
解決:稀釋HRP至少10倍。
5、信號(hào)太弱或沒有?
原因:
1.太多的HRP積聚導(dǎo)致信號(hào)快速的消退;
2.抗原或抗體的濃度不夠;
3.轉(zhuǎn)印不成功;
4.HRP或其他底物的效價(jià)降低。
解決:
1.稀釋HRP至少10倍;
2.增加其濃度;
3.尋找合適的轉(zhuǎn)移方法和條件;
4.選擇其他的合適底物。
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