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FAM azide| FAM N3|FAM疊氮化物

FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel.

With versatility of Click chemistry and this reagent, it is possible to attach this popular fluorophore to nearly any alkyne bearing molecule.

FAM azide is available both as solid compound, and as 10 mM solution in DMSO ready to use in our recommended labeling protocol. This product is a pure 5-isomer. FAM is a replacement for Alexa Fluor 488, DyLight 488.

FAM azide| FAM N3|FAM疊氮化物

General properties：

Appearance:	yellowish crystals
Molecular weight:	458.42
Molecular formula:	C₂₄H₁₈N₄O₆
Solubility:	soluble in polar organic solvents (DMF, DMSO, alcohols)
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Spectral properties：

Excitation maximum, nm:	494
ε, L⋅mol⁻¹⋅cm⁻¹:	75000
Emission maximum, nm:	520
Fluorescence quantum yield:	0.9
CF₂₆₀:	0.20
CF₂₈₀:	0.17

熒光染料示例	激發(fā)光
Indo-1, Ca saturated	331 nm
Indo-1 Ca2+	346 nm
Cascade Blue BSA pH 7.0	401 nm
Cascade Blue	398 nm
LysoTracker Blue	373 nm
Alexa 405	401 nm
LysoSensor Blue pH 5.0	374 nm
LysoSensor Blue	374 nm
DyLight 405	399 nm
DyLight 350	332 nm
BFP (Blue Fluorescent Protein)	380 nm
Alexa 350	343 nm
7-Amino-4-methylcoumarin pH 7.0	346 nm
Amino Coumarin	345 nm
AMCA conjugate	347 nm
Coumarin	360 nm
7-Hydroxy-4-methylcoumarin	360 nm
7-Hydroxy-4-methylcoumarin pH 9.0	361 nm
6,8-Difluoro-7-hydroxy-4-methylcoumarin pH 9.0	358 nm
Hoechst 33342	352 nm
Pacific Blue	404 nm
Hoechst 33258	352 nm
Hoechst 33258-DNA	352 nm
Pacific Blue antibody conjugate pH 8.0	404 nm
PO-PRO-1	434 nm
PO-PRO-1-DNA	435 nm
POPO-1	433 nm
POPO-1-DNA	433 nm
DAPI-DNA	359 nm
DAPI	358 nm
Marina Blue	362 nm
SYTOX Blue-DNA	445 nm
CFP (Cyan Fluorescent Protein)	434 nm
eCFP (Enhanced Cyan Fluorescent Protein)	437 nm
1-Anilinonaphthalene-8-sulfonic acid (1,8-ANS)	375 nm
Indo-1, Ca free	346 nm
1,8-ANS (1-Anilinonaphthalene-8-sulfonic acid)	375 nm
BO-PRO-1-DNA	462 nm
BOPRO-1	462 nm
BOBO-1-DNA	461 nm
SYTO 45-DNA	451 nm
evoglow-Pp1	448 nm
evoglow-Bs1	448 nm
evoglow-Bs2	448 nm
Auramine O	431 nm
DiO	487 nm
LysoSensor Green pH 5.0	447 nm
Cy 2	489 nm
LysoSensor Green	447 nm
Fura-2, high Ca	336 nm
Fura-2 Ca2+sup>	336 nm
SYTO 13-DNA	488 nm
YO-PRO-1-DNA	491 nm
YOYO-1-DNA	491 nm
eGFP (Enhanced Green Fluorescent Protein)	488 nm
LysoTracker Green	503 nm
GFP (S65T)	489 nm
BODIPY FL, MeOH	502 nm
Sapphire	396 nm
BODIPY FL conjugate	503 nm
MitoTracker Green	490 nm
MitoTracker Green FM, MeOH	490 nm
Fluorescein 0.1 M NaOH	493 nm
Calcein pH 9.0	494 nm
Fluorescein pH 9.0	490 nm
Calcein	493 nm
Fura-2, no Ca	367 nm
Fluo-4	494 nm
FDA	495 nm
DTAF	495 nm
Fluorescein	495 nm
Fluorescein antibody conjugate pH 8.0	493 nm
CFDA	495 nm
FITC	495 nm
Alexa Fluor 488 hydrazide-water	493 nm
DyLight 488	493 nm
5-FAM pH 9.0	492 nm
FITC antibody conjugate pH 8.0	495 nm
Alexa 488	493 nm
Rhodamine 110	497 nm
Rhodamine 110 pH 7.0	497 nm
Acridine Orange	431 nm
Alexa Fluor 488 antibody conjugate pH 8.0	499 nm
BCECF pH 5.5	485 nm
PicoGreendsDNA quantitation reagent	502 nm
SYBR Green I	498 nm
Rhodaminen Green pH 7.0	497 nm
CyQUANT GR-DNA	502 nm
NeuroTrace 500/525, green fluorescent Nissl stain-RNA	497 nm
DansylCadaverine	335 nm
Rhodol Green antibody conjugate pH 8.0	499 nm
Fluoro-Emerald	495 nm
Nissl	497 nm
Fluorescein dextran pH 8.0	501 nm
Rhodamine Green	497 nm
5-(and-6)-Carboxy-2', 7'-dichlorofluorescein pH 9.0	504 nm
DansylCadaverine, MeOH	335 nm
eYFP (Enhanced Yellow Fluorescent Protein)	514 nm
Oregon Green 488	498 nm
Oregon Green 488 antibody conjugate pH 8.0	498 nm
Fluo-3	506 nm
BCECF pH 9.0	501 nm
SBFI-Na+	336 nm
Fluo-3 Ca2+	506 nm
Rhodamine 123, MeOH	507 nm
FlAsH	509 nm
Calcium Green-1 Ca2+	506 nm
Magnesium Green	507 nm
DM-NERF pH 4.0	493 nm
Calcium Green	506 nm
Citrine	515 nm
LysoSensor Yellow pH 9.0	335 nm
TO-PRO-1-DNA	515 nm
Magnesium Green Mg2+	507 nm
Sodium Green Na+	507 nm
TOTO-1-DNA	514 nm
Oregon Green 514	512 nm
Oregon Green 514 antibody conjugate pH 8.0	513 nm
NBD-X	466 nm
DM-NERF pH 7.0	509 nm
NBD-X, MeOH	467 nm
CI-NERF pH 6.0	513 nm
Alexa 430	431 nm
Alexa Fluor 430 antibody conjugate pH 7.2	431 nm
CI-NERF pH 2.5	504 nm
Lucifer Yellow, CH	428 nm
LysoSensor Yellow pH 3.0	389 nm
6-TET, SE pH 9.0	521 nm
Eosin antibody conjugate pH 8.0	525 nm
Eosin	524 nm
6-Carboxyrhodamine 6G pH 7.0	526 nm
6-Carboxyrhodamine 6G, hydrochloride	525 nm
Bodipy R6G SE	528 nm
BODIPY R6G, MeOH	528 nm
6 JOE	520 nm
Cascade Yellow antibody conjugate pH 8.0	399 nm
Cascade Yellow	399 nm
mBanana	540 nm
Alexa Fluor 532 antibody conjugate pH 7.2	528 nm
Alexa 532	528 nm
Erythrosin-5-isothiocyanate pH 9.0	533 nm
6-HEX, SE pH 9.0	534 nm
mOrange	548 nm
mHoneydew	478 nm
Cy 3	549 nm
Rhodamine B	543 nm
DiI	551 nm
5-TAMRA-MeOH	543 nm
Alexa 555	553 nm
Alexa Fluor 555 antibody conjugate pH 7.2	553 nm
DyLight 549	555 nm
BODIPY TMR-X, SE	544 nm
BODIPY TMR-X, MeOH	544 nm
PO-PRO-3-DNA	539 nm
PO-PRO-3	539 nm
Rhodamine	551 nm
Bodipy TMR-X conjugate	544 nm
POPO-3	533 nm
Alexa 546	562 nm
BODIPY TMR-X antibody conjugate pH 7.2	544 nm
Calcium Orange Ca2+	549 nm
TRITC	550 nm
Calcium Orange	549 nm
Rhodaminephalloidin pH 7.0	558 nm
MitoTracker Orange	551 nm
MitoTracker Orange, MeOH	551 nm
Phycoerythrin	565 nm
Magnesium Orange	550 nm
R-Phycoerythrin pH 7.5	565 nm
5-TAMRA pH 7.0	553 nm
5-TAMRA	549 nm
Rhod-2	552 nm
FM 1-43	472 nm
Rhod-2 Ca2+	553 nm
Tetramethylrhodamine antibody conjugate pH 8.0	552 nm
FM 1-43 lipid	473 nm
LOLO-1-DNA	568 nm
dTomato	554 nm
DsRed	563 nm
Dapoxyl (2-aminoethyl) sulfonamide	372 nm
Tetramethylrhodamine dextran pH 7.0	555 nm
Fluor-Ruby	554 nm
Resorufin	571 nm
Resorufin pH 9.0	571 nm
mTangerine	568 nm
LysoTracker Red	578 nm
Lissaminerhodamine	572 nm
Cy 3.5	578 nm
Rhodamine Red-X antibody conjugate pH 8.0	573 nm
Sulforhodamine 101, EtOH	578 nm
JC-1 pH 8.2	593 nm
JC-1	592 nm
mStrawberry	575 nm
MitoTracker Red	578 nm
MitoTracker Red, MeOH	578 nm
X-Rhod-1 Ca2+	580 nm
Alexa Fluor 568 antibody conjugate pH 7.2	579 nm
Alexa 568	576 nm
5-ROX pH 7.0	578 nm
5-ROX (5-Carboxy-X-rhodamine, triethylammonium salt)	578 nm
BO-PRO-3-DNA	574 nm
BOPRO-3	574 nm
BOBO-3-DNA	570 nm
Ethidium Bromide	524 nm
ReAsH	597 nm
Calcium Crimson	589 nm
Calcium Crimson Ca2+	590 nm
mRFP	585 nm
mCherry	587 nm
Texas Red-X antibody conjugate pH 7.2	596 nm
HcRed	590 nm
DyLight 594	592 nm
Ethidium homodimer-1-DNA	528 nm
Ethidiumhomodimer	528 nm
Propidium Iodide	538 nm
SYPRO Ruby	467 nm
Propidium Iodide-DNA	538 nm
Alexa 594	590 nm
BODIPY TR-X, SE	588 nm
BODIPY TR-X, MeOH	588 nm
BODIPY TR-X phallacidin pH 7.0	590 nm
Alexa Fluor 610 R-phycoerythrin streptavidin pH 7.2	567 nm
YO-PRO-3-DNA	613 nm
Di-8 ANEPPS	469 nm
Di-8-ANEPPS-lipid	469 nm
YOYO-3-DNA	612 nm
Nile Red-lipid	553 nm
Nile Red	559 nm
DyLight 633	624 nm
mPlum	587 nm
TO-PRO-3-DNA	642 nm
DDAO pH 9.0	648 nm
Fura Red, high Ca	434 nm
Allophycocyanin pH 7.5	651 nm
APC (allophycocyanin)	650 nm
Nile Blue, EtOH	631 nm
TOTO-3-DNA	642 nm
Cy 5	646 nm
BODIPY 650/665-X, MeOH	646 nm
Alexa Fluor 647 R-phycoerythrin streptavidin pH 7.2	569 nm
DyLight 649	652 nm
Alexa Fluor 647 antibody conjugate pH 7.2	653 nm
Alexa 647	653 nm
Fura Red Ca2+	435 nm
Atto 647	644 nm
Fura Red, low Ca	472 nm
Carboxynaphthofluorescein pH 10.0	600 nm
Alexa 660	664 nm
Alexa Fluor 660 antibody conjugate pH 7.2	663 nm
Cy 5.5	673 nm
Alexa Fluor 680 antibody conjugate pH 7.2	679 nm
Alexa 680	679 nm
DyLight 680	678 nm
Alexa Fluor 700 antibody conjugate pH 7.2	696 nm
Alexa 700	696 nm
FM 4-64, 2% CHAPS	506 nm
FM 4-64	508 nm