上海研盟生物有限公司*品質(zhì)供應(yīng)Anti-Cyclin T1抗體,我公司銷售國內(nèi)外科研產(chǎn)品,如生物試劑、生化試劑,一抗二抗,各種標(biāo)記抗體,Elisa酶聯(lián)免疫試劑盒,放免試劑盒,培養(yǎng)基,各種動(dòng)物血清等產(chǎn)品,歡迎廣大新老客戶前來咨詢訂購,訂購:或。
周期素依賴性激酶9抗體,Anti-Cyclin T1抗體簡單介紹:
【中文名稱】:周期素依賴性激酶9抗體
【英文名稱】:Anti-Cyclin T1抗體
【英文別名】:C 2k; C-2K; CDC2 related kinase; CDC2L4; Cdk 9; Cdk9; CDK9_HUMAN; Cell division cycle 2 like protein kinase 4; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; CTK1; Cyclin dependent kinase 9; Cyclin-dependent kinase 9; PITALRE; Serine threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; TAK; Tat associated kinase complex catalytic subunit.
【規(guī) 格】:0.1ml/0.2ml
【相關(guān)標(biāo)記】:Alexa Fluor 350、Alexa Fluor 488、Alexa Fluor 555、Alexa Fluor 647、AP、APC、Biotin、Cy3、Cy5、Cy5.5、Cy7、FITC、Gold、HRP、PE、PE-Cy3、PE-CY5、PE-CY5.5、PE-CY7、RBITC.【濃 度】:1mg/1ml
【抗體來源】:Rabbit or Mouse or Goat
【克隆類型】:polyclonal or monoclonal
【產(chǎn)品類型】:一抗
【產(chǎn)品應(yīng)用】:可以用于做石蠟切片免疫組化,冰凍切片免疫組化,Elisa,WB,免疫熒光、流式細(xì)胞術(shù)等實(shí)驗(yàn)。
周期素依賴性激酶9抗體【背景介紹】:The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
周期素依賴性激酶9抗體,Anti-Cyclin T1抗體免疫組化操作流程:
(一) 儀器設(shè)備
1. 18cm不銹鋼高壓鍋或電爐或用微波爐.
2. 水浴鍋
(二) 試 劑
1. PBS緩沖液(ph7.2―7.4):NaC137mmol/L,KCl 2.7mmol/L ,Na2HPO4 4.3mmol/L,
KH2PO4 1.4mmol/L.
2.0.01mol/L檸檬酸鈉緩沖液(CB,ph6.0,1000ml):檸檬酸三鈉3g,檸檬酸0.4g。
3.0.5mol/L EDTA緩沖液(ph8.0):700ml水中溶解186.1g EDTA&
8226;2H2O,用10mmol/L
NaOH調(diào)至ph8.0,加水至1000ml.
4. 1mol/L的TBS緩沖液(ph8.0):在800ml水中溶解121gTris堿,用1N的HCl調(diào)至ph8.0,加水1000ml。
5. 酶消化液:a. 0.1%*:用0.1%CaCl 12(ph7.8)配制。b.0.4%*液:用0.1N的HCl配制。
6. 3%甲醇―H2O2溶液:用30%H2O2和80%甲醇溶液配制
7. 風(fēng)裱劑:a. 甘油和0.5mmol/L碳酸鹽緩沖液(ph9.0–9.5)等量混合
b 油和TBS(PBS)配制
8.TBS/PBS PH9.0–9.5,適用于熒光纖維鏡標(biāo)本;ph7.0-7.4適合光學(xué)纖維標(biāo)本。
(三) 操作流程
1 脫蠟和水化:脫蠟前應(yīng)將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘。 a 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b 無水乙醇中浸泡五分鐘
c 95%乙醇中浸泡五分鐘
d 75%乙醇中浸泡五分鐘
2 抗原修復(fù):用于福爾馬林固定的石蠟包埋組織芯片
★研盟生物 ★合作: ★客服
相關(guān)標(biāo)記抗體:HRP標(biāo)記抗體,Biotin標(biāo)記抗體,Gold標(biāo)記抗體,RBITC標(biāo)記抗體,AP標(biāo)記抗體,FITC標(biāo)記抗體,Cy3標(biāo)記抗體,Cy5標(biāo)記抗體,Cy5.5標(biāo)記抗體,Cy7標(biāo)記抗體,PE標(biāo)記抗體,PE-Cy3標(biāo)記抗體,PE-Cy5標(biāo)記抗體,PE-Cy5.5標(biāo)記抗體,PE-Cy7標(biāo)記抗體,APC標(biāo)記抗體,Alexa Fluor 350標(biāo)記抗體,Alexa Fluor 488標(biāo)記抗體,Alexa Fluor 555標(biāo)記抗體,Alexa Fluor 647標(biāo)記抗體
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