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上海研盟生物科技有限公司Anti-AARS2抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細胞術(shù)等實驗中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取。客服
英文名稱:Anti-AARS2 antibody
中文名稱:丙氨酰tRNA合成酶2抗體
丙氨酰tRNA合成酶2抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
★研盟生物★丙氨酰tRNA合成酶2抗體,Anti-AARS2抗體
石蠟切片的免疫組化SP法參考步驟:
1.60℃烤片2小時
2.脫蠟。二甲苯脫蠟15分鐘,三次
3.水化。依次經(jīng)過無水乙醇、95%、90%、80%、70%,各5分鐘,蒸餾水(或自來水)5分鐘
4.PBS洗5分鐘,三次
5.抗原修復(fù)。枸櫞酸緩沖液(約92-95℃)煮沸(沸水浴)修復(fù)15分鐘,自然涼至室溫。PBS洗5分鐘,三次
6.3%雙氧水消化內(nèi)源性的過氧化物酶,20分鐘,PBS洗5分鐘,三次
7.正常山羊血清封閉,37℃20分鐘
8.一抗孵育,4℃過ye.
9.PBS洗5分鐘,三次
10.二抗孵育,37℃20分鐘(二抗為*標(biāo)記的羊抗兔IgG),PBS洗5分鐘,三次
11.三抗孵育,37℃20分鐘(三抗為辣根酶標(biāo)記的鏈霉親和素),PBS洗5分鐘,三次
12.DAB顯色,鏡下觀察結(jié)果,適時終止
13.蘇木素復(fù)染5分鐘,自來水沖洗片刻,1%的鹽酸酒精(75%)溶液(請自行配置,配制比例為:100ml 75%酒精+1ml濃鹽酸)分化30秒,自來水沖洗5分鐘藍化
14.脫水。依次經(jīng)過70%、80%、90%、95%、無水乙醇,各5分鐘,二甲苯15分鐘,三次
15.封片,中性樹膠封片
丙氨酰tRNA合成酶2抗體,Anti-AARS2抗體溶解方法:
方法1:我們的抗體(凍干粉)已經(jīng)包含了0.01M PBS、1% BSA和0.1% 防腐劑(疊氮鈉或*),您只需要加入滅菌的雙蒸水就行了??贵w溶解后,置于-20℃保存,分裝后使用,避免反復(fù)凍融,可保證至少1年有效;
方法2:也可以只加入一半體積的雙蒸水,再用一半體積的甘油補足,置于-20℃保存,這樣抗體不會凍,有利于抗體的穩(wěn)定。
后續(xù)試驗時,再使用對應(yīng)的緩沖液稀釋成工作液,即用即配。
我們的抗體(凍干粉),在常溫放置下,至少一個月有效;若在-20℃下保存(*),至少三年有效。
Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:
1) Reconstituting by directly adding 100uL of sterile, distilled water.
2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.
簡單介紹:
產(chǎn)品別名:alanyl tRNA synthetase 2; AARSL; bA444E17.1; Gm89; MT ALARS; MTALARS ; AARS 2; AARS2; Alanine tRNA ligase 2 mitochondrial; Alanine tRNA ligase; Alanyl tRNA synthetase 2 mitochondrial; Alanyl tRNA synthetase like; AlaRS; KIAA1270; MGC69820; Probable alanyl tRNA synthetase mitochondrial; RP11-444E17.1.
抗體來源:Rabbit or Mouse
保質(zhì)期:1年
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetases play critical roles in mRNA translation by charging tRNAs with their cognate amino acids. The encoded protein is a mitochondrial enzyme that specifically aminoacylates alanyl-tRNA. Mutations in this gene are a cause of combined oxidative phosphorylation deficiency 8.
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