上海研盟生物科技有限公司Anti-iNOS抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?/span>
英文名稱:Anti-iNOS antibody
中文名稱:一氧化氮合成酶-2(誘導(dǎo)型)抗體
一氧化氮合成酶-2(誘導(dǎo)型)抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
一氧化氮合成酶-2(誘導(dǎo)型)抗體,Anti-iNOS抗體抗原的分類:
一. 免疫學(xué)分類:
(1)*抗原、半抗原;
(2)胸腺依賴抗原與非依賴抗原。
二. 臨床分類:
(1)外源性抗原:微生物、花粉等;
(2)內(nèi)源性抗原:隱蔽的自身抗原、腫瘤相關(guān)抗原等;
(3)同種異型抗原:人類白細(xì)胞抗原、血型抗原;
(4)異嗜性抗原:人與其他動(dòng)物、植物等之間存在的共同抗原。
★研盟生物★一氧化氮合成酶-2(誘導(dǎo)型)抗體,Anti-iNOS抗體
免疫組化結(jié)果的判斷:
對免疫組化結(jié)果的判斷應(yīng)持科學(xué)的慎重態(tài)度,要準(zhǔn)確判斷陽性和陰性,排除假陽性和假陰性結(jié)果,必須嚴(yán)格對照實(shí)驗(yàn),對新發(fā)現(xiàn)的陽性結(jié)果,除有對照實(shí)驗(yàn)結(jié)果之外,應(yīng)進(jìn)行多次重復(fù)實(shí)驗(yàn),可用幾種方法進(jìn)行驗(yàn)證。必須學(xué)會(huì)判斷特異性染色和非特異性染色,對初學(xué)者更為重要,否則會(huì)得出不科學(xué)的結(jié)論。特異性染色和非特異性染色的鑒別點(diǎn)主要在于特異性反應(yīng)產(chǎn)物常分布于特定的部位。如胞漿內(nèi),也有分布在細(xì)胞核和細(xì)胞表面的,即具有結(jié)構(gòu)性。特異性染色表現(xiàn)為在同一切片上呈現(xiàn)不同程度的陽性染色結(jié)果。非特異性染色表現(xiàn)為無一定的分布規(guī)律,常呈某一部位成片的均勻著色,細(xì)胞和周圍的結(jié)締組織均無區(qū)別的著色,或結(jié)締組織呈現(xiàn)很強(qiáng)的染色。非特異性染色常出現(xiàn)在干燥切片的邊緣,有刀痕或者折疊的部位。在過大的組織塊,中心固定不良也會(huì)導(dǎo)致非特異性染色。有時(shí)可見非特異性染色和特異性染色同時(shí)存在,由于過強(qiáng)的非特異性染色背景不但影響對特異性染色結(jié)果的觀察和記錄,而且令人對其特異性結(jié)果產(chǎn)生懷疑。
免疫組化的呈色深淺可反映抗原存在的數(shù)量,可作為定性、定位和定量的依據(jù)。
1.陽性細(xì)胞染色分布有三種類型:細(xì)胞漿、細(xì)胞核、細(xì)胞膜表面。大部分抗原見于細(xì)胞漿,可見于整個(gè)胞漿或部分胞漿
2.陽性細(xì)胞分布可分為灶型和彌漫性
3.由于細(xì)胞內(nèi)含抗原量不同,所以染色強(qiáng)度不一。如果細(xì)胞之間染色強(qiáng)度相同,常提示其反應(yīng)為非特異性
4.陽性細(xì)胞染色定位于單個(gè)細(xì)胞,且與陰性細(xì)胞相互交雜分布;而非特異性染色常不限于單個(gè)細(xì)胞,而是累及一片細(xì)胞。
5.切片邊緣、刀痕或褶皺區(qū)域,壞死或擠壓的細(xì)胞區(qū),膠原結(jié)締組織等,常表現(xiàn)為相同的陽性染色強(qiáng)度,不能用于判斷陽性。
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相關(guān)標(biāo)記抗體:HRP標(biāo)記抗體,Biotin標(biāo)記抗體,Gold標(biāo)記抗體,RBITC標(biāo)記抗體,AP標(biāo)記抗體,FITC標(biāo)記抗體,Cy3標(biāo)記抗體,Cy5標(biāo)記抗體,Cy5.5標(biāo)記抗體,Cy7標(biāo)記抗體,PE標(biāo)記抗體,PE-Cy3標(biāo)記抗體,PE-Cy5標(biāo)記抗體,PE-Cy5.5標(biāo)記抗體,PE-Cy7標(biāo)記抗體,APC標(biāo)記抗體,Alexa Fluor 350標(biāo)記抗體,Alexa Fluor 488標(biāo)記抗體,Alexa Fluor 555標(biāo)記抗體,Alexa Fluor 647標(biāo)記抗體
簡單介紹:
產(chǎn)品別名:i NOS; Nitric Oxide Synthase, Inducible; HEP NOS; Hepatocyte NOS; HEPNOS; Inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; INOS; Inosl; MAC NOS; Macrophage NOS; Nitric oxide synthase 2 inducible macrophage; Nitric oxide synthase 2A (inducible hepatocytes); Nitric oxide synthase inducible; NOS 2; NOS 2A; NOS; Nos II; NOS type II; Nos2; NOS2A; NOS2_HUMAN.
抗體來源:Rabbit or Mouse
保質(zhì)期:1年
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).
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