上海研盟生物科技有限公司Anti-SMMHC抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說(shuō)明書(shū)隨貨發(fā)送,您也可以直接我司在線(xiàn)客服索取。客服
英文名稱(chēng):Anti-SMMHC antibody
中文名稱(chēng):平滑肌肌球蛋白重鏈抗體
平滑肌肌球蛋白重鏈抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
平滑肌肌球蛋白重鏈抗體,Anti-SMMHC抗體抗原的分類(lèi):
一. 免疫學(xué)分類(lèi):
(1)*抗原、半抗原;
(2)胸腺依賴(lài)抗原與非依賴(lài)抗原。
二. 臨床分類(lèi):
(1)外源性抗原:微生物、花粉等;
(2)內(nèi)源性抗原:隱蔽的自身抗原、腫瘤相關(guān)抗原等;
(3)同種異型抗原:人類(lèi)白細(xì)胞抗原、血型抗原;
(4)異嗜性抗原:人與其他動(dòng)物、植物等之間存在的共同抗原。
★研盟生物★平滑肌肌球蛋白重鏈抗體,Anti-SMMHC抗體
免疫組化結(jié)果的判斷:
對(duì)免疫組化結(jié)果的判斷應(yīng)持科學(xué)的慎重態(tài)度,要準(zhǔn)確判斷陽(yáng)性和陰性,排除假陽(yáng)性和假陰性結(jié)果,必須嚴(yán)格對(duì)照實(shí)驗(yàn),對(duì)新發(fā)現(xiàn)的陽(yáng)性結(jié)果,除有對(duì)照實(shí)驗(yàn)結(jié)果之外,應(yīng)進(jìn)行多次重復(fù)實(shí)驗(yàn),可用幾種方法進(jìn)行驗(yàn)證。必須學(xué)會(huì)判斷特異性染色和非特異性染色,對(duì)初學(xué)者更為重要,否則會(huì)得出不科學(xué)的結(jié)論。特異性染色和非特異性染色的鑒別點(diǎn)主要在于特異性反應(yīng)產(chǎn)物常分布于特定的部位。如胞漿內(nèi),也有分布在細(xì)胞核和細(xì)胞表面的,即具有結(jié)構(gòu)性。特異性染色表現(xiàn)為在同一切片上呈現(xiàn)不同程度的陽(yáng)性染色結(jié)果。非特異性染色表現(xiàn)為無(wú)一定的分布規(guī)律,常呈某一部位成片的均勻著色,細(xì)胞和周?chē)慕Y(jié)締組織均無(wú)區(qū)別的著色,或結(jié)締組織呈現(xiàn)很強(qiáng)的染色。非特異性染色常出現(xiàn)在干燥切片的邊緣,有刀痕或者折疊的部位。在過(guò)大的組織塊,中心固定不良也會(huì)導(dǎo)致非特異性染色。有時(shí)可見(jiàn)非特異性染色和特異性染色同時(shí)存在,由于過(guò)強(qiáng)的非特異性染色背景不但影響對(duì)特異性染色結(jié)果的觀(guān)察和記錄,而且令人對(duì)其特異性結(jié)果產(chǎn)生懷疑。
免疫組化的呈色深淺可反映抗原存在的數(shù)量,可作為定性、定位和定量的依據(jù)。
1.陽(yáng)性細(xì)胞染色分布有三種類(lèi)型:細(xì)胞漿、細(xì)胞核、細(xì)胞膜表面。大部分抗原見(jiàn)于細(xì)胞漿,可見(jiàn)于整個(gè)胞漿或部分胞漿
2.陽(yáng)性細(xì)胞分布可分為灶型和彌漫性
3.由于細(xì)胞內(nèi)含抗原量不同,所以染色強(qiáng)度不一。如果細(xì)胞之間染色強(qiáng)度相同,常提示其反應(yīng)為非特異性
4.陽(yáng)性細(xì)胞染色定位于單個(gè)細(xì)胞,且與陰性細(xì)胞相互交雜分布;而非特異性染色常不限于單個(gè)細(xì)胞,而是累及一片細(xì)胞。
5.切片邊緣、刀痕或褶皺區(qū)域,壞死或擠壓的細(xì)胞區(qū),膠原結(jié)締組織等,常表現(xiàn)為相同的陽(yáng)性染色強(qiáng)度,不能用于判斷陽(yáng)性。
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簡(jiǎn)單介紹:
產(chǎn)品別名:SMMHC; Myosin 11; Myosin-11; Myosin, smooth muscle, heavy chain; Myosin Smooth Muscle; AAT 4; AAT4; DKFZp686D10126; DKFZp686D19237; FAA 4; FAA4; FLJ35232; KIAA0866; MGC126726; MGC32963; MYH 11; MYH11 protein; Myosin heavy chain 11 smooth muscle; Myosin heavy chain smooth muscle isoform; Myosin heavy polypeptide 11 smooth muscle; Myosin11; SMHC; Smooth muscle myosin heavy chain isoform SM1; MYH11_HUMAN.
抗體來(lái)源:Rabbit or Mouse
保質(zhì)期:1年
克隆類(lèi)型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16[inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified.
MYH11 is a smooth muscle myosin belonging to the myosin heavy chain family. It is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP.
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